Robust detection of undifferentiated iPSC among differentiated cells

Abstract Recent progress in human induced pluripotent stem cells (iPSC) technologies suggest that iPSC application in regenerative medicine is a closer reality. Numerous challenges prevent iPSC application in the development of numerous tissues and for the treatment of various diseases. A key concer...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Keisuke Sekine, Syusaku Tsuzuki, Ryota Yasui, Tatsuya Kobayashi, Kazuki Ikeda, Yuki Hamada, Eriko Kanai, J. Gray Camp, Barbara Treutlein, Yasuharu Ueno, Satoshi Okamoto, Hideki Taniguchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6b046a1bf42e4e869b7d0c6f73f4af8e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6b046a1bf42e4e869b7d0c6f73f4af8e
record_format	dspace
spelling	oai:doaj.org-article:6b046a1bf42e4e869b7d0c6f73f4af8e2021-12-02T17:44:55ZRobust detection of undifferentiated iPSC among differentiated cells10.1038/s41598-020-66845-62045-2322https://doaj.org/article/6b046a1bf42e4e869b7d0c6f73f4af8e2020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-66845-6https://doaj.org/toc/2045-2322Abstract Recent progress in human induced pluripotent stem cells (iPSC) technologies suggest that iPSC application in regenerative medicine is a closer reality. Numerous challenges prevent iPSC application in the development of numerous tissues and for the treatment of various diseases. A key concern in therapeutic applications is the safety of the cell products to be transplanted into patients. Here, we present novel method for detecting residual undifferentiated iPSCs amongst directed differentiated cells of all three germ lineages. Marker genes, which are expressed specifically and highly in undifferentiated iPSC, were selected from single cell RNA sequence data to perform robust and sensitive detection of residual undifferentiated cells in differentiated cell products. ESRG (Embryonic Stem Cell Related), CNMD (Chondromodulin), and SFRP2 (Secreted Frizzled Related Protein 2) were well-correlated with the actual amounts of residual undifferentiated cells and could be used to detect residual cells in a highly sensitive manner using qPCR. In addition, such markers could be used to detect residual undifferentiated cells from various differentiated cells, including hepatic cells and pancreatic cells for the endodermal lineage, endothelial cells and mesenchymal cells for the mesodermal lineage, and neural cells for the ectodermal lineage. Our method facilitates robust validation and could enhance the safety of the cell products through the exclusion of undifferentiated iPSC.Keisuke SekineSyusaku TsuzukiRyota YasuiTatsuya KobayashiKazuki IkedaYuki HamadaEriko KanaiJ. Gray CampBarbara TreutleinYasuharu UenoSatoshi OkamotoHideki TaniguchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keisuke Sekine Syusaku Tsuzuki Ryota Yasui Tatsuya Kobayashi Kazuki Ikeda Yuki Hamada Eriko Kanai J. Gray Camp Barbara Treutlein Yasuharu Ueno Satoshi Okamoto Hideki Taniguchi Robust detection of undifferentiated iPSC among differentiated cells
description	Abstract Recent progress in human induced pluripotent stem cells (iPSC) technologies suggest that iPSC application in regenerative medicine is a closer reality. Numerous challenges prevent iPSC application in the development of numerous tissues and for the treatment of various diseases. A key concern in therapeutic applications is the safety of the cell products to be transplanted into patients. Here, we present novel method for detecting residual undifferentiated iPSCs amongst directed differentiated cells of all three germ lineages. Marker genes, which are expressed specifically and highly in undifferentiated iPSC, were selected from single cell RNA sequence data to perform robust and sensitive detection of residual undifferentiated cells in differentiated cell products. ESRG (Embryonic Stem Cell Related), CNMD (Chondromodulin), and SFRP2 (Secreted Frizzled Related Protein 2) were well-correlated with the actual amounts of residual undifferentiated cells and could be used to detect residual cells in a highly sensitive manner using qPCR. In addition, such markers could be used to detect residual undifferentiated cells from various differentiated cells, including hepatic cells and pancreatic cells for the endodermal lineage, endothelial cells and mesenchymal cells for the mesodermal lineage, and neural cells for the ectodermal lineage. Our method facilitates robust validation and could enhance the safety of the cell products through the exclusion of undifferentiated iPSC.
format	article
author	Keisuke Sekine Syusaku Tsuzuki Ryota Yasui Tatsuya Kobayashi Kazuki Ikeda Yuki Hamada Eriko Kanai J. Gray Camp Barbara Treutlein Yasuharu Ueno Satoshi Okamoto Hideki Taniguchi
author_facet	Keisuke Sekine Syusaku Tsuzuki Ryota Yasui Tatsuya Kobayashi Kazuki Ikeda Yuki Hamada Eriko Kanai J. Gray Camp Barbara Treutlein Yasuharu Ueno Satoshi Okamoto Hideki Taniguchi
author_sort	Keisuke Sekine
title	Robust detection of undifferentiated iPSC among differentiated cells
title_short	Robust detection of undifferentiated iPSC among differentiated cells
title_full	Robust detection of undifferentiated iPSC among differentiated cells
title_fullStr	Robust detection of undifferentiated iPSC among differentiated cells
title_full_unstemmed	Robust detection of undifferentiated iPSC among differentiated cells
title_sort	robust detection of undifferentiated ipsc among differentiated cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/6b046a1bf42e4e869b7d0c6f73f4af8e
work_keys_str_mv	AT keisukesekine robustdetectionofundifferentiatedipscamongdifferentiatedcells AT syusakutsuzuki robustdetectionofundifferentiatedipscamongdifferentiatedcells AT ryotayasui robustdetectionofundifferentiatedipscamongdifferentiatedcells AT tatsuyakobayashi robustdetectionofundifferentiatedipscamongdifferentiatedcells AT kazukiikeda robustdetectionofundifferentiatedipscamongdifferentiatedcells AT yukihamada robustdetectionofundifferentiatedipscamongdifferentiatedcells AT erikokanai robustdetectionofundifferentiatedipscamongdifferentiatedcells AT jgraycamp robustdetectionofundifferentiatedipscamongdifferentiatedcells AT barbaratreutlein robustdetectionofundifferentiatedipscamongdifferentiatedcells AT yasuharuueno robustdetectionofundifferentiatedipscamongdifferentiatedcells AT satoshiokamoto robustdetectionofundifferentiatedipscamongdifferentiatedcells AT hidekitaniguchi robustdetectionofundifferentiatedipscamongdifferentiatedcells
_version_	1718379592646918144

Robust detection of undifferentiated iPSC among differentiated cells

Ejemplares similares