Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites

Abstract Box C/D ribonucleoprotein complexes are RNA-guided methyltransferases that methylate the ribose 2’-OH of RNA. The central ‘guide RNA’ has box C and D motifs at its ends, which are crucial for activity. Archaeal guide RNAs have a second box C’/D’ motif pair that is also essential for functio...

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Autores principales:	Simone Höfler, Peer Lukat, Wulf Blankenfeldt, Teresa Carlomagno
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6b06d43f8c7b44b0bfbeb0e72c36370a
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spelling	oai:doaj.org-article:6b06d43f8c7b44b0bfbeb0e72c36370a2021-12-02T19:04:11ZEukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites10.1038/s41598-021-97030-y2045-2322https://doaj.org/article/6b06d43f8c7b44b0bfbeb0e72c36370a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97030-yhttps://doaj.org/toc/2045-2322Abstract Box C/D ribonucleoprotein complexes are RNA-guided methyltransferases that methylate the ribose 2’-OH of RNA. The central ‘guide RNA’ has box C and D motifs at its ends, which are crucial for activity. Archaeal guide RNAs have a second box C’/D’ motif pair that is also essential for function. This second motif is poorly conserved in eukaryotes and its function is uncertain. Conflicting literature data report that eukaryotic box C’/D’ motifs do or do not bind proteins specialized to recognize box C/D-motifs and are or are not important for function. Despite this uncertainty, the architecture of eukaryotic 2’-O-methylation enzymes is thought to be similar to that of their archaeal counterpart. Here, we use biochemistry, X-ray crystallography and mutant analysis to demonstrate the absence of functional box C’/D’ motifs in more than 80% of yeast guide RNAs. We conclude that eukaryotic Box C/D RNPs have two non-symmetric protein assembly sites and that their three-dimensional architecture differs from that of archaeal 2’-O-methylation enzymes.Simone HöflerPeer LukatWulf BlankenfeldtTeresa CarlomagnoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simone Höfler Peer Lukat Wulf Blankenfeldt Teresa Carlomagno Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites
description	Abstract Box C/D ribonucleoprotein complexes are RNA-guided methyltransferases that methylate the ribose 2’-OH of RNA. The central ‘guide RNA’ has box C and D motifs at its ends, which are crucial for activity. Archaeal guide RNAs have a second box C’/D’ motif pair that is also essential for function. This second motif is poorly conserved in eukaryotes and its function is uncertain. Conflicting literature data report that eukaryotic box C’/D’ motifs do or do not bind proteins specialized to recognize box C/D-motifs and are or are not important for function. Despite this uncertainty, the architecture of eukaryotic 2’-O-methylation enzymes is thought to be similar to that of their archaeal counterpart. Here, we use biochemistry, X-ray crystallography and mutant analysis to demonstrate the absence of functional box C’/D’ motifs in more than 80% of yeast guide RNAs. We conclude that eukaryotic Box C/D RNPs have two non-symmetric protein assembly sites and that their three-dimensional architecture differs from that of archaeal 2’-O-methylation enzymes.
format	article
author	Simone Höfler Peer Lukat Wulf Blankenfeldt Teresa Carlomagno
author_facet	Simone Höfler Peer Lukat Wulf Blankenfeldt Teresa Carlomagno
author_sort	Simone Höfler
title	Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites
title_short	Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites
title_full	Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites
title_fullStr	Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites
title_full_unstemmed	Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites
title_sort	eukaryotic box c/d methylation machinery has two non-symmetric protein assembly sites
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6b06d43f8c7b44b0bfbeb0e72c36370a
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Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites

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