Automated solid-phase subcloning based on beads brought into proximity by magnetic force.
In the fields of proteomics, metabolic engineering and synthetic biology there is a need for high-throughput and reliable cloning methods to facilitate construction of expression vectors and genetic pathways. Here, we describe a new approach for solid-phase cloning in which both the vector and the g...
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Main Authors: | Elton P Hudson, Andrej Nikoshkov, Mathias Uhlen, Johan Rockberg |
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Format: | article |
Language: | EN |
Published: |
Public Library of Science (PLoS)
2012
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Online Access: | https://doaj.org/article/6b08f8dde85e454b8bdb2569e17ca94e |
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