Automated solid-phase subcloning based on beads brought into proximity by magnetic force.

In the fields of proteomics, metabolic engineering and synthetic biology there is a need for high-throughput and reliable cloning methods to facilitate construction of expression vectors and genetic pathways. Here, we describe a new approach for solid-phase cloning in which both the vector and the g...

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Bibliographic Details
Main Authors: Elton P Hudson, Andrej Nikoshkov, Mathias Uhlen, Johan Rockberg
Format: article
Language:EN
Published: Public Library of Science (PLoS) 2012
Subjects:
R
Q
Online Access:https://doaj.org/article/6b08f8dde85e454b8bdb2569e17ca94e
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