Effect of Oral Administration of Weissella confusa on Fecal and Plasma Ethanol Concentrations, Lipids and Glucose Metabolism in Wistar Rats Fed High Fructose and Fat Diet

Fouad MF Elshaghabee,1 Darab Ghadimi,1 Diana Habermann,1 Michael de Vrese,1 Wilhelm Bockelmann,1 Hans-Jürgen Kaatsch,2 Knut J Heller,1 Jürgen Schrezenmeir3,4 1Max Rubner-Institute, Federal Research Institute of Nutrition and Food, Department of Microbiology and Biotechnology, Kiel,...

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Autores principales:	Elshaghabee FMF, Ghadimi D, Habermann D, de Vrese M, Bockelmann W, Kaatsch HJ, Heller KJ, Schrezenmeir J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	high fructose-high fat diet fecal ethanol fecal short chain fatty acids lipidemia glycemia nafld Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/6b0f303360ef4d66b19e375fcb896f88
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spelling	oai:doaj.org-article:6b0f303360ef4d66b19e375fcb896f882021-12-02T09:14:49ZEffect of Oral Administration of Weissella confusa on Fecal and Plasma Ethanol Concentrations, Lipids and Glucose Metabolism in Wistar Rats Fed High Fructose and Fat Diet1179-1535https://doaj.org/article/6b0f303360ef4d66b19e375fcb896f882020-06-01T00:00:00Zhttps://www.dovepress.com/effect-of-oral-administration-of-weissella-confusa-on-fecal-and-plasma-peer-reviewed-article-HMERhttps://doaj.org/toc/1179-1535Fouad MF Elshaghabee,1 Darab Ghadimi,1 Diana Habermann,1 Michael de Vrese,1 Wilhelm Bockelmann,1 Hans-Jürgen Kaatsch,2 Knut J Heller,1 Jürgen Schrezenmeir3,4 1Max Rubner-Institute, Federal Research Institute of Nutrition and Food, Department of Microbiology and Biotechnology, Kiel, Germany; 2Institute of Legal Medicine, University Hospital of Schleswig-Holstein, Kiel, Germany; 3Max Rubner-Institute, Federal Research Institute of Nutrition and Food, Department of Physiology and Biochemistry of Nutrition, Karlsruhe, Germany; 4Clinical Research Center, Kiel Innovation and Technology Center, Kiel, GermanyCorrespondence: Darab Ghadimi Tel +49 4316092512Fax +49 4316092472Email darab.ghadimi@mri.bund.deBackground and Purpose: In previous investigations, Weissella confusa was shown to lack the metabolic pathway from fructose to mannitol and to produce ethanol when cultivated in the presence of fructose. Hence, we assessed the effect of oral administration of W. confusa (strain NRRL-B-14171) on blood and fecal ethanol concentrations, glucose and lipid metabolism and traits of the metabolic syndrome in Wistar rats (n=27) fed diets with two different fat and fructose levels and with or without the addition of W. confusa during a total intervention time of 15 weeks (105 days).Materials and Methods: From week 1 to 6, rats were given a medium fructose and fat (MFru-MF) diet containing 28% fructose and 10% fat without the addition of W. confusa (control group, n=13) or mixed with 30 g per kg diet of lyophilized W. confusa (10.56 ± 0.20 log CFU/g; W. confusa group, n=14). From week 7 to 15, the percentage of dietary fructose and fat in the control and W. confusa group was increased to 56% and 16%, respectively (high fructose-high fat (HFru-HF) diet).Results: In HFru-HF-fed rats, W. confusa was detected in feces, regardless of whether W. confusa was added to the diet or not, but not in rats receiving the MFru-MF diet without added W. confusa or in an additional control group (n=10) fed standard rat food without fructose, increased fat content and W. confusa. This indicates that fecal W. confusa may be derived from orally administered W. confusa as well as – in the case of high fructose and fat intake and obesity of rats – from the intestinal microbiota. As shown by multifactorial ANOVA, blood ethanol, the relative liver weight, serum triglycerides, and serum cholesterol as well as fecal ethanol, ADH, acetate, propionate and butyrate, but not lactate, were significantly higher in the W. confusa – compared to the control group.Discussion: This is the first in vivo trial demonstrating that heterofermentative lactic acid bacteria lacking the mannitol pathway (like W. confusa) can increase fecal and blood ethanol concentrations in mammals on a high fructose-high fat diet. This may explain why W. confusa resulted in hyperlipidemia and may promote development of NAFLD in the host.Keywords: high fructose-high fat diet, fecal ethanol, fecal short-chain fatty acids, lipidemia, glycemia, NAFLDElshaghabee FMFGhadimi DHabermann Dde Vrese MBockelmann WKaatsch HJHeller KJSchrezenmeir JDove Medical Pressarticlehigh fructose-high fat dietfecal ethanolfecal short chain fatty acidslipidemiaglycemianafldDiseases of the digestive system. GastroenterologyRC799-869ENHepatic Medicine: Evidence and Research, Vol Volume 12, Pp 93-106 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	high fructose-high fat diet fecal ethanol fecal short chain fatty acids lipidemia glycemia nafld Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	high fructose-high fat diet fecal ethanol fecal short chain fatty acids lipidemia glycemia nafld Diseases of the digestive system. Gastroenterology RC799-869 Elshaghabee FMF Ghadimi D Habermann D de Vrese M Bockelmann W Kaatsch HJ Heller KJ Schrezenmeir J Effect of Oral Administration of Weissella confusa on Fecal and Plasma Ethanol Concentrations, Lipids and Glucose Metabolism in Wistar Rats Fed High Fructose and Fat Diet
description	Fouad MF Elshaghabee,1 Darab Ghadimi,1 Diana Habermann,1 Michael de Vrese,1 Wilhelm Bockelmann,1 Hans-Jürgen Kaatsch,2 Knut J Heller,1 Jürgen Schrezenmeir3,4 1Max Rubner-Institute, Federal Research Institute of Nutrition and Food, Department of Microbiology and Biotechnology, Kiel, Germany; 2Institute of Legal Medicine, University Hospital of Schleswig-Holstein, Kiel, Germany; 3Max Rubner-Institute, Federal Research Institute of Nutrition and Food, Department of Physiology and Biochemistry of Nutrition, Karlsruhe, Germany; 4Clinical Research Center, Kiel Innovation and Technology Center, Kiel, GermanyCorrespondence: Darab Ghadimi Tel +49 4316092512Fax +49 4316092472Email darab.ghadimi@mri.bund.deBackground and Purpose: In previous investigations, Weissella confusa was shown to lack the metabolic pathway from fructose to mannitol and to produce ethanol when cultivated in the presence of fructose. Hence, we assessed the effect of oral administration of W. confusa (strain NRRL-B-14171) on blood and fecal ethanol concentrations, glucose and lipid metabolism and traits of the metabolic syndrome in Wistar rats (n=27) fed diets with two different fat and fructose levels and with or without the addition of W. confusa during a total intervention time of 15 weeks (105 days).Materials and Methods: From week 1 to 6, rats were given a medium fructose and fat (MFru-MF) diet containing 28% fructose and 10% fat without the addition of W. confusa (control group, n=13) or mixed with 30 g per kg diet of lyophilized W. confusa (10.56 ± 0.20 log CFU/g; W. confusa group, n=14). From week 7 to 15, the percentage of dietary fructose and fat in the control and W. confusa group was increased to 56% and 16%, respectively (high fructose-high fat (HFru-HF) diet).Results: In HFru-HF-fed rats, W. confusa was detected in feces, regardless of whether W. confusa was added to the diet or not, but not in rats receiving the MFru-MF diet without added W. confusa or in an additional control group (n=10) fed standard rat food without fructose, increased fat content and W. confusa. This indicates that fecal W. confusa may be derived from orally administered W. confusa as well as – in the case of high fructose and fat intake and obesity of rats – from the intestinal microbiota. As shown by multifactorial ANOVA, blood ethanol, the relative liver weight, serum triglycerides, and serum cholesterol as well as fecal ethanol, ADH, acetate, propionate and butyrate, but not lactate, were significantly higher in the W. confusa – compared to the control group.Discussion: This is the first in vivo trial demonstrating that heterofermentative lactic acid bacteria lacking the mannitol pathway (like W. confusa) can increase fecal and blood ethanol concentrations in mammals on a high fructose-high fat diet. This may explain why W. confusa resulted in hyperlipidemia and may promote development of NAFLD in the host.Keywords: high fructose-high fat diet, fecal ethanol, fecal short-chain fatty acids, lipidemia, glycemia, NAFLD
format	article
author	Elshaghabee FMF Ghadimi D Habermann D de Vrese M Bockelmann W Kaatsch HJ Heller KJ Schrezenmeir J
author_facet	Elshaghabee FMF Ghadimi D Habermann D de Vrese M Bockelmann W Kaatsch HJ Heller KJ Schrezenmeir J
author_sort	Elshaghabee FMF
title	Effect of Oral Administration of Weissella confusa on Fecal and Plasma Ethanol Concentrations, Lipids and Glucose Metabolism in Wistar Rats Fed High Fructose and Fat Diet
title_short	Effect of Oral Administration of Weissella confusa on Fecal and Plasma Ethanol Concentrations, Lipids and Glucose Metabolism in Wistar Rats Fed High Fructose and Fat Diet
title_full	Effect of Oral Administration of Weissella confusa on Fecal and Plasma Ethanol Concentrations, Lipids and Glucose Metabolism in Wistar Rats Fed High Fructose and Fat Diet
title_fullStr	Effect of Oral Administration of Weissella confusa on Fecal and Plasma Ethanol Concentrations, Lipids and Glucose Metabolism in Wistar Rats Fed High Fructose and Fat Diet
title_full_unstemmed	Effect of Oral Administration of Weissella confusa on Fecal and Plasma Ethanol Concentrations, Lipids and Glucose Metabolism in Wistar Rats Fed High Fructose and Fat Diet
title_sort	effect of oral administration of weissella confusa on fecal and plasma ethanol concentrations, lipids and glucose metabolism in wistar rats fed high fructose and fat diet
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/6b0f303360ef4d66b19e375fcb896f88
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Effect of Oral Administration of Weissella confusa on Fecal and Plasma Ethanol Concentrations, Lipids and Glucose Metabolism in Wistar Rats Fed High Fructose and Fat Diet

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