Cardiac mitofusin-1 is reduced in non-responding patients with idiopathic dilated cardiomyopathy
Abstract Prognosis of severe heart failure remains poor. Urgent new therapies are required. Some heart failure patients do not respond to established multidisciplinary treatment and are classified as “non-responders”. The outcome is especially poor for non-responders, and underlying mechanisms are l...
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Nature Portfolio
2021
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oai:doaj.org-article:6b102410e463408695d87fe8621b2bd02021-12-02T17:04:34ZCardiac mitofusin-1 is reduced in non-responding patients with idiopathic dilated cardiomyopathy10.1038/s41598-021-86209-y2045-2322https://doaj.org/article/6b102410e463408695d87fe8621b2bd02021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86209-yhttps://doaj.org/toc/2045-2322Abstract Prognosis of severe heart failure remains poor. Urgent new therapies are required. Some heart failure patients do not respond to established multidisciplinary treatment and are classified as “non-responders”. The outcome is especially poor for non-responders, and underlying mechanisms are largely unknown. Mitofusin-1 (Mfn1), a mitochondrial fusion protein, is significantly reduced in non-responding patients. This study aimed to elucidate the role of Mfn1 in the failing heart. Twenty-two idiopathic dilated cardiomyopathy (IDCM) patients who underwent endomyocardial biopsy of intraventricular septum were included. Of the 22 patients, 8 were non-responders (left ventricular (LV) ejection fraction (LVEF) of < 10% improvement at late phase follow-up). Electron microscopy (EM), quantitative PCR, and immunofluorescence studies were performed to explore the biological processes and molecules involved in failure to respond. Studies in cardiac specific Mfn1 knockout mice (c-Mfn1 KO), and in vitro studies with neonatal rat ventricular myocytes (NRVMs) were also conducted. A significant reduction in mitochondrial size in cardiomyocytes, and Mfn1, was observed in non-responders. A LV pressure overload with thoracic aortic constriction (TAC) c-Mfn1 KO mouse model was generated. Systolic function was reduced in c-Mfn1 KO mice, while mitochondria alteration in TAC c-Mfn1 KO mice increased. In vitro studies in NRVMs indicated negative regulation of Mfn1 by the β-AR/cAMP/PKA/miR-140-5p pathway resulting in significant reduction in mitochondrial respiration of NRVMs. The level of miR140-5p was increased in cardiac tissues of non-responders. Mfn1 is a biomarker of heart failure in non-responders. Therapies targeting mitochondrial dynamics and homeostasis are next generation therapy for non-responding heart failure patients.Yung Ting HsiaoIppei ShimizuTakayuki WakasugiYohko YoshidaRyutaro IkegamiYuka HayashiMasayoshi SudaGoro KatsuumiMasaaki NakaoTakuya OzawaDaisuke IzumiTakeshi KashimuraKazuyuki OzakiTomoyoshi SogaTohru MinaminoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Yung Ting Hsiao Ippei Shimizu Takayuki Wakasugi Yohko Yoshida Ryutaro Ikegami Yuka Hayashi Masayoshi Suda Goro Katsuumi Masaaki Nakao Takuya Ozawa Daisuke Izumi Takeshi Kashimura Kazuyuki Ozaki Tomoyoshi Soga Tohru Minamino Cardiac mitofusin-1 is reduced in non-responding patients with idiopathic dilated cardiomyopathy |
| description |
Abstract Prognosis of severe heart failure remains poor. Urgent new therapies are required. Some heart failure patients do not respond to established multidisciplinary treatment and are classified as “non-responders”. The outcome is especially poor for non-responders, and underlying mechanisms are largely unknown. Mitofusin-1 (Mfn1), a mitochondrial fusion protein, is significantly reduced in non-responding patients. This study aimed to elucidate the role of Mfn1 in the failing heart. Twenty-two idiopathic dilated cardiomyopathy (IDCM) patients who underwent endomyocardial biopsy of intraventricular septum were included. Of the 22 patients, 8 were non-responders (left ventricular (LV) ejection fraction (LVEF) of < 10% improvement at late phase follow-up). Electron microscopy (EM), quantitative PCR, and immunofluorescence studies were performed to explore the biological processes and molecules involved in failure to respond. Studies in cardiac specific Mfn1 knockout mice (c-Mfn1 KO), and in vitro studies with neonatal rat ventricular myocytes (NRVMs) were also conducted. A significant reduction in mitochondrial size in cardiomyocytes, and Mfn1, was observed in non-responders. A LV pressure overload with thoracic aortic constriction (TAC) c-Mfn1 KO mouse model was generated. Systolic function was reduced in c-Mfn1 KO mice, while mitochondria alteration in TAC c-Mfn1 KO mice increased. In vitro studies in NRVMs indicated negative regulation of Mfn1 by the β-AR/cAMP/PKA/miR-140-5p pathway resulting in significant reduction in mitochondrial respiration of NRVMs. The level of miR140-5p was increased in cardiac tissues of non-responders. Mfn1 is a biomarker of heart failure in non-responders. Therapies targeting mitochondrial dynamics and homeostasis are next generation therapy for non-responding heart failure patients. |
| format |
article |
| author |
Yung Ting Hsiao Ippei Shimizu Takayuki Wakasugi Yohko Yoshida Ryutaro Ikegami Yuka Hayashi Masayoshi Suda Goro Katsuumi Masaaki Nakao Takuya Ozawa Daisuke Izumi Takeshi Kashimura Kazuyuki Ozaki Tomoyoshi Soga Tohru Minamino |
| author_facet |
Yung Ting Hsiao Ippei Shimizu Takayuki Wakasugi Yohko Yoshida Ryutaro Ikegami Yuka Hayashi Masayoshi Suda Goro Katsuumi Masaaki Nakao Takuya Ozawa Daisuke Izumi Takeshi Kashimura Kazuyuki Ozaki Tomoyoshi Soga Tohru Minamino |
| author_sort |
Yung Ting Hsiao |
| title |
Cardiac mitofusin-1 is reduced in non-responding patients with idiopathic dilated cardiomyopathy |
| title_short |
Cardiac mitofusin-1 is reduced in non-responding patients with idiopathic dilated cardiomyopathy |
| title_full |
Cardiac mitofusin-1 is reduced in non-responding patients with idiopathic dilated cardiomyopathy |
| title_fullStr |
Cardiac mitofusin-1 is reduced in non-responding patients with idiopathic dilated cardiomyopathy |
| title_full_unstemmed |
Cardiac mitofusin-1 is reduced in non-responding patients with idiopathic dilated cardiomyopathy |
| title_sort |
cardiac mitofusin-1 is reduced in non-responding patients with idiopathic dilated cardiomyopathy |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/6b102410e463408695d87fe8621b2bd0 |
| work_keys_str_mv |
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