Characterization of the Curcuminoids Fingerprint Profile in Curcuma and Zingiber Genera by TLC – Digital Image Analysis

Curcuma longa, C. xanthorrhiza, C. heyneana, and Zingiber cassumunar contain high curcuminoid and have relatively the similar yellow color. Therefore, that they are potentially adulterated and difficult to differentiate in the form of powder. Hence, it is necessary to characterize the fingerprints c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anisa Lailatusy Syarifah, Rurini Retnowati, Hermin Sulistyarti
Formato:	article
Lenguaje:	EN
Publicado:	University of Brawijaya 2019
Materias:	curcuma zingiber curcuminoid thin-layer chromatography grey value rgb Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/6b106fbafd34405f831b8858d2d29eba
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6b106fbafd34405f831b8858d2d29eba
record_format	dspace
spelling	oai:doaj.org-article:6b106fbafd34405f831b8858d2d29eba2021-12-02T16:37:08ZCharacterization of the Curcuminoids Fingerprint Profile in Curcuma and Zingiber Genera by TLC – Digital Image Analysis2302-46902302-4690https://doaj.org/article/6b106fbafd34405f831b8858d2d29eba2019-06-01T00:00:00Zhttps://jpacr.ub.ac.id/index.php/jpacr/article/view/462/pdfhttps://doaj.org/toc/2302-4690https://doaj.org/toc/2302-4690Curcuma longa, C. xanthorrhiza, C. heyneana, and Zingiber cassumunar contain high curcuminoid and have relatively the similar yellow color. Therefore, that they are potentially adulterated and difficult to differentiate in the form of powder. Hence, it is necessary to characterize the fingerprints compound profile by a simple and rapid method. This research aims to determine fingerprint compound profile of curcuminoid using Thin Layer Chromatography (TLC) and digital image analysis. The result of the research identified that the fingerprint compound profile of curcuminoid on the four rhizomes was obtained by TLC method using silica gel 60 GF254 as the stationary phase, chloroform:dichloromethane:methanol (13:6:1) as the mobile phase, and observation under UV 254 nm light and citroborate reagent. Thereafter, the digital image analysis was carried out using Image J software according to the gray value and % of RGB (red-green-blue) value. Based on gray value and % of RGB, both Curcuma and Zingiber genera were differentiated through curcumin compound (Rf 0.63), demethoxycurcumin (Rf 0.34), bisdemethoxycurcumin (Rf 0.21). The profile of fingerprint compound on Curcuma longa, C. xanthorrhiza, C. heyneana, and Zingiber cassumunar was differentiated through Rf 0.26; Rf 0.17; and Rf 0.10.Anisa Lailatusy SyarifahRurini RetnowatiHermin SulistyartiUniversity of Brawijayaarticlecurcumazingibercurcuminoidthin-layer chromatographygrey valuergbChemistryQD1-999ENJournal of Pure and Applied Chemistry Research, Vol 8, Iss 2, Pp 147-161 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	curcuma zingiber curcuminoid thin-layer chromatography grey value rgb Chemistry QD1-999
spellingShingle	curcuma zingiber curcuminoid thin-layer chromatography grey value rgb Chemistry QD1-999 Anisa Lailatusy Syarifah Rurini Retnowati Hermin Sulistyarti Characterization of the Curcuminoids Fingerprint Profile in Curcuma and Zingiber Genera by TLC – Digital Image Analysis
description	Curcuma longa, C. xanthorrhiza, C. heyneana, and Zingiber cassumunar contain high curcuminoid and have relatively the similar yellow color. Therefore, that they are potentially adulterated and difficult to differentiate in the form of powder. Hence, it is necessary to characterize the fingerprints compound profile by a simple and rapid method. This research aims to determine fingerprint compound profile of curcuminoid using Thin Layer Chromatography (TLC) and digital image analysis. The result of the research identified that the fingerprint compound profile of curcuminoid on the four rhizomes was obtained by TLC method using silica gel 60 GF254 as the stationary phase, chloroform:dichloromethane:methanol (13:6:1) as the mobile phase, and observation under UV 254 nm light and citroborate reagent. Thereafter, the digital image analysis was carried out using Image J software according to the gray value and % of RGB (red-green-blue) value. Based on gray value and % of RGB, both Curcuma and Zingiber genera were differentiated through curcumin compound (Rf 0.63), demethoxycurcumin (Rf 0.34), bisdemethoxycurcumin (Rf 0.21). The profile of fingerprint compound on Curcuma longa, C. xanthorrhiza, C. heyneana, and Zingiber cassumunar was differentiated through Rf 0.26; Rf 0.17; and Rf 0.10.
format	article
author	Anisa Lailatusy Syarifah Rurini Retnowati Hermin Sulistyarti
author_facet	Anisa Lailatusy Syarifah Rurini Retnowati Hermin Sulistyarti
author_sort	Anisa Lailatusy Syarifah
title	Characterization of the Curcuminoids Fingerprint Profile in Curcuma and Zingiber Genera by TLC – Digital Image Analysis
title_short	Characterization of the Curcuminoids Fingerprint Profile in Curcuma and Zingiber Genera by TLC – Digital Image Analysis
title_full	Characterization of the Curcuminoids Fingerprint Profile in Curcuma and Zingiber Genera by TLC – Digital Image Analysis
title_fullStr	Characterization of the Curcuminoids Fingerprint Profile in Curcuma and Zingiber Genera by TLC – Digital Image Analysis
title_full_unstemmed	Characterization of the Curcuminoids Fingerprint Profile in Curcuma and Zingiber Genera by TLC – Digital Image Analysis
title_sort	characterization of the curcuminoids fingerprint profile in curcuma and zingiber genera by tlc – digital image analysis
publisher	University of Brawijaya
publishDate	2019
url	https://doaj.org/article/6b106fbafd34405f831b8858d2d29eba
work_keys_str_mv	AT anisalailatusysyarifah characterizationofthecurcuminoidsfingerprintprofileincurcumaandzingibergenerabytlcdigitalimageanalysis AT ruriniretnowati characterizationofthecurcuminoidsfingerprintprofileincurcumaandzingibergenerabytlcdigitalimageanalysis AT herminsulistyarti characterizationofthecurcuminoidsfingerprintprofileincurcumaandzingibergenerabytlcdigitalimageanalysis
_version_	1718383638117089280

Characterization of the Curcuminoids Fingerprint Profile in Curcuma and Zingiber Genera by TLC – Digital Image Analysis

Ejemplares similares