Comparison of Chilean honeys through MALDI-TOF-MS profiling and evaluation of their antioxidant and antibacterial potential

Honey is the most famous natural sweet substance produced by honeybees (Apis mellifera). It contains numerous functional compounds, from which health benefits are obtained. In Chile, the production of honey is associated with its unique biodiversity, but it is exported mostly, as bulk honey. This wo...

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Autores principales:	Verónica R. Olate-Olave, Luis Guzmán, Xaviera A. López-Cortés, Rafael Cornejo, Fabiane M. Nachtigall, Marnix Doorn, Leonardo Silva Santos, Arturo Bejarano
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	MALDI-TOF-MS Fingerprints Honey Antioxidant activity Antibacterial activity Medicinal honey Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/6b11be3fdb164e49bd19f0866cc42236
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spelling	oai:doaj.org-article:6b11be3fdb164e49bd19f0866cc422362021-12-02T04:59:04ZComparison of Chilean honeys through MALDI-TOF-MS profiling and evaluation of their antioxidant and antibacterial potential0570-178310.1016/j.aoas.2021.11.001https://doaj.org/article/6b11be3fdb164e49bd19f0866cc422362021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0570178321000397https://doaj.org/toc/0570-1783Honey is the most famous natural sweet substance produced by honeybees (Apis mellifera). It contains numerous functional compounds, from which health benefits are obtained. In Chile, the production of honey is associated with its unique biodiversity, but it is exported mostly, as bulk honey. This work aimed to characterize the chemical and biological properties of Chilean honey on a large scale. The use of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) technique, combined with multivariate statistical analysis was introduced to study the chemical profiles of polyfloral honey. The use of a complementary mass spectrometry method allowed the identification of 25 different constituents in the studied honey, including hydrocarbons, acids, esters, glycoside isoprenoids, ketones, and a dihydroxyflavanone. The evaluation of biological properties in Chilean honey was measured in a representative number of polyfloral samples. For this purpose, the total phenolics and flavonoid content were measured in the selected samples. The antioxidant activity of the honey was evaluated through the ferric reducing antioxidant power assay (FRAP) and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. On the other hand, the antibacterial activity was assessed by the dilution technique to determine the minimum inhibitory concentration (MIC) of the honey against seven bacterial strains, including Gram-positive and Gram-negative bacteria. The results demonstrated that the antioxidant and antibacterial activities of Chilean honey are variable and they could have similar properties in comparison with other well-recognized bioactive honey.Verónica R. Olate-OlaveLuis GuzmánXaviera A. López-CortésRafael CornejoFabiane M. NachtigallMarnix DoornLeonardo Silva SantosArturo BejaranoElsevierarticleMALDI-TOF-MSFingerprintsHoneyAntioxidant activityAntibacterial activityMedicinal honeyAgriculture (General)S1-972ENAnnals of Agricultural Sciences, Vol 66, Iss 2, Pp 152-161 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MALDI-TOF-MS Fingerprints Honey Antioxidant activity Antibacterial activity Medicinal honey Agriculture (General) S1-972
spellingShingle	MALDI-TOF-MS Fingerprints Honey Antioxidant activity Antibacterial activity Medicinal honey Agriculture (General) S1-972 Verónica R. Olate-Olave Luis Guzmán Xaviera A. López-Cortés Rafael Cornejo Fabiane M. Nachtigall Marnix Doorn Leonardo Silva Santos Arturo Bejarano Comparison of Chilean honeys through MALDI-TOF-MS profiling and evaluation of their antioxidant and antibacterial potential
description	Honey is the most famous natural sweet substance produced by honeybees (Apis mellifera). It contains numerous functional compounds, from which health benefits are obtained. In Chile, the production of honey is associated with its unique biodiversity, but it is exported mostly, as bulk honey. This work aimed to characterize the chemical and biological properties of Chilean honey on a large scale. The use of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) technique, combined with multivariate statistical analysis was introduced to study the chemical profiles of polyfloral honey. The use of a complementary mass spectrometry method allowed the identification of 25 different constituents in the studied honey, including hydrocarbons, acids, esters, glycoside isoprenoids, ketones, and a dihydroxyflavanone. The evaluation of biological properties in Chilean honey was measured in a representative number of polyfloral samples. For this purpose, the total phenolics and flavonoid content were measured in the selected samples. The antioxidant activity of the honey was evaluated through the ferric reducing antioxidant power assay (FRAP) and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. On the other hand, the antibacterial activity was assessed by the dilution technique to determine the minimum inhibitory concentration (MIC) of the honey against seven bacterial strains, including Gram-positive and Gram-negative bacteria. The results demonstrated that the antioxidant and antibacterial activities of Chilean honey are variable and they could have similar properties in comparison with other well-recognized bioactive honey.
format	article
author	Verónica R. Olate-Olave Luis Guzmán Xaviera A. López-Cortés Rafael Cornejo Fabiane M. Nachtigall Marnix Doorn Leonardo Silva Santos Arturo Bejarano
author_facet	Verónica R. Olate-Olave Luis Guzmán Xaviera A. López-Cortés Rafael Cornejo Fabiane M. Nachtigall Marnix Doorn Leonardo Silva Santos Arturo Bejarano
author_sort	Verónica R. Olate-Olave
title	Comparison of Chilean honeys through MALDI-TOF-MS profiling and evaluation of their antioxidant and antibacterial potential
title_short	Comparison of Chilean honeys through MALDI-TOF-MS profiling and evaluation of their antioxidant and antibacterial potential
title_full	Comparison of Chilean honeys through MALDI-TOF-MS profiling and evaluation of their antioxidant and antibacterial potential
title_fullStr	Comparison of Chilean honeys through MALDI-TOF-MS profiling and evaluation of their antioxidant and antibacterial potential
title_full_unstemmed	Comparison of Chilean honeys through MALDI-TOF-MS profiling and evaluation of their antioxidant and antibacterial potential
title_sort	comparison of chilean honeys through maldi-tof-ms profiling and evaluation of their antioxidant and antibacterial potential
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/6b11be3fdb164e49bd19f0866cc42236
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Comparison of Chilean honeys through MALDI-TOF-MS profiling and evaluation of their antioxidant and antibacterial potential

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