Raman Spectroscopy for Rapid Evaluation of Surgical Margins during Breast Cancer Lumpectomy

Abstract Failure to precisely distinguish malignant from healthy tissue has severe implications for breast cancer surgical outcomes. Clinical prognoses depend on precisely distinguishing healthy from malignant tissue during surgery. Laser Raman spectroscopy (LRS) has been previously shown to differe...

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Autores principales:	Willie C. Zúñiga, Veronica Jones, Sarah M. Anderson, Alex Echevarria, Nathaniel L. Miller, Connor Stashko, Daniel Schmolze, Philip D. Cha, Ragini Kothari, Yuman Fong, Michael C. Storrie-Lombardi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6b11c7736f184d34bbc3a967e226cd9e
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spelling	oai:doaj.org-article:6b11c7736f184d34bbc3a967e226cd9e2021-12-02T15:09:21ZRaman Spectroscopy for Rapid Evaluation of Surgical Margins during Breast Cancer Lumpectomy10.1038/s41598-019-51112-02045-2322https://doaj.org/article/6b11c7736f184d34bbc3a967e226cd9e2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51112-0https://doaj.org/toc/2045-2322Abstract Failure to precisely distinguish malignant from healthy tissue has severe implications for breast cancer surgical outcomes. Clinical prognoses depend on precisely distinguishing healthy from malignant tissue during surgery. Laser Raman spectroscopy (LRS) has been previously shown to differentiate benign from malignant tissue in real time. However, the cost, assembly effort, and technical expertise needed for construction and implementation of the technique have prohibited widespread adoption. Recently, Raman spectrometers have been developed for non-medical uses and have become commercially available and affordable. Here we demonstrate that this current generation of Raman spectrometers can readily identify cancer in breast surgical specimens. We evaluated two commercially available, portable, near-infrared Raman systems operating at excitation wavelengths of either 785 nm or 1064 nm, collecting a total of 164 Raman spectra from cancerous, benign, and transitional regions of resected breast tissue from six patients undergoing mastectomy. The spectra were classified using standard multivariate statistical techniques. We identified a minimal set of spectral bands sufficient to reliably distinguish between healthy and malignant tissue using either the 1064 nm or 785 nm system. Our results indicate that current generation Raman spectrometers can be used as a rapid diagnostic technique distinguishing benign from malignant tissue during surgery.Willie C. ZúñigaVeronica JonesSarah M. AndersonAlex EchevarriaNathaniel L. MillerConnor StashkoDaniel SchmolzePhilip D. ChaRagini KothariYuman FongMichael C. Storrie-LombardiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-16 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Willie C. Zúñiga Veronica Jones Sarah M. Anderson Alex Echevarria Nathaniel L. Miller Connor Stashko Daniel Schmolze Philip D. Cha Ragini Kothari Yuman Fong Michael C. Storrie-Lombardi Raman Spectroscopy for Rapid Evaluation of Surgical Margins during Breast Cancer Lumpectomy
description	Abstract Failure to precisely distinguish malignant from healthy tissue has severe implications for breast cancer surgical outcomes. Clinical prognoses depend on precisely distinguishing healthy from malignant tissue during surgery. Laser Raman spectroscopy (LRS) has been previously shown to differentiate benign from malignant tissue in real time. However, the cost, assembly effort, and technical expertise needed for construction and implementation of the technique have prohibited widespread adoption. Recently, Raman spectrometers have been developed for non-medical uses and have become commercially available and affordable. Here we demonstrate that this current generation of Raman spectrometers can readily identify cancer in breast surgical specimens. We evaluated two commercially available, portable, near-infrared Raman systems operating at excitation wavelengths of either 785 nm or 1064 nm, collecting a total of 164 Raman spectra from cancerous, benign, and transitional regions of resected breast tissue from six patients undergoing mastectomy. The spectra were classified using standard multivariate statistical techniques. We identified a minimal set of spectral bands sufficient to reliably distinguish between healthy and malignant tissue using either the 1064 nm or 785 nm system. Our results indicate that current generation Raman spectrometers can be used as a rapid diagnostic technique distinguishing benign from malignant tissue during surgery.
format	article
author	Willie C. Zúñiga Veronica Jones Sarah M. Anderson Alex Echevarria Nathaniel L. Miller Connor Stashko Daniel Schmolze Philip D. Cha Ragini Kothari Yuman Fong Michael C. Storrie-Lombardi
author_facet	Willie C. Zúñiga Veronica Jones Sarah M. Anderson Alex Echevarria Nathaniel L. Miller Connor Stashko Daniel Schmolze Philip D. Cha Ragini Kothari Yuman Fong Michael C. Storrie-Lombardi
author_sort	Willie C. Zúñiga
title	Raman Spectroscopy for Rapid Evaluation of Surgical Margins during Breast Cancer Lumpectomy
title_short	Raman Spectroscopy for Rapid Evaluation of Surgical Margins during Breast Cancer Lumpectomy
title_full	Raman Spectroscopy for Rapid Evaluation of Surgical Margins during Breast Cancer Lumpectomy
title_fullStr	Raman Spectroscopy for Rapid Evaluation of Surgical Margins during Breast Cancer Lumpectomy
title_full_unstemmed	Raman Spectroscopy for Rapid Evaluation of Surgical Margins during Breast Cancer Lumpectomy
title_sort	raman spectroscopy for rapid evaluation of surgical margins during breast cancer lumpectomy
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/6b11c7736f184d34bbc3a967e226cd9e
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Raman Spectroscopy for Rapid Evaluation of Surgical Margins during Breast Cancer Lumpectomy

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