A possible pathogenetic factor of sickle-cell disease based on fluorescent analysis via an optofluidic resonator

Abstract Waveguide based optofluidic resonator features high precision and high sensitivity in real-time fluorescent analysis. We present a novel optofluidic resonator following the hollow-core metal-cladding waveguide structure, which is then used to record the real-time binding process of Fe2+ and...

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Autores principales:	Hailang Dai, Cheng Yin, Xiaona Ye, Bei Jiang, Maowu Ran, Zhuangqi Cao, Xianfeng Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6b11fb5d03de4e3d967475eb212f99bf
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spelling	oai:doaj.org-article:6b11fb5d03de4e3d967475eb212f99bf2021-12-02T11:52:39ZA possible pathogenetic factor of sickle-cell disease based on fluorescent analysis via an optofluidic resonator10.1038/s41598-017-03634-82045-2322https://doaj.org/article/6b11fb5d03de4e3d967475eb212f99bf2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03634-8https://doaj.org/toc/2045-2322Abstract Waveguide based optofluidic resonator features high precision and high sensitivity in real-time fluorescent analysis. We present a novel optofluidic resonator following the hollow-core metal-cladding waveguide structure, which is then used to record the real-time binding process of Fe2+ and Fe3+ with protoporphyrin IX (PpIX) in PBS solution, respectively. The central fluorescent wavelength of compound with Fe2+ is in good accordance with that of the normal hemoglobin, whilst the peaks of the Fe3+ compound match the hemoglobin specimen from sickle-cell disease (SCD) patients. Similar statement holds when we monitor the real-time oxidation processes of these products by injecting oxygen into the optofluidic chip. These observations lead to the speculation that the SCD is caused by replacing the Fe2+ in hemoglobin with Fe3+, which may be insightful in the discovery of new clinical routes to cure this disease.Hailang DaiCheng YinXiaona YeBei JiangMaowu RanZhuangqi CaoXianfeng ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hailang Dai Cheng Yin Xiaona Ye Bei Jiang Maowu Ran Zhuangqi Cao Xianfeng Chen A possible pathogenetic factor of sickle-cell disease based on fluorescent analysis via an optofluidic resonator
description	Abstract Waveguide based optofluidic resonator features high precision and high sensitivity in real-time fluorescent analysis. We present a novel optofluidic resonator following the hollow-core metal-cladding waveguide structure, which is then used to record the real-time binding process of Fe2+ and Fe3+ with protoporphyrin IX (PpIX) in PBS solution, respectively. The central fluorescent wavelength of compound with Fe2+ is in good accordance with that of the normal hemoglobin, whilst the peaks of the Fe3+ compound match the hemoglobin specimen from sickle-cell disease (SCD) patients. Similar statement holds when we monitor the real-time oxidation processes of these products by injecting oxygen into the optofluidic chip. These observations lead to the speculation that the SCD is caused by replacing the Fe2+ in hemoglobin with Fe3+, which may be insightful in the discovery of new clinical routes to cure this disease.
format	article
author	Hailang Dai Cheng Yin Xiaona Ye Bei Jiang Maowu Ran Zhuangqi Cao Xianfeng Chen
author_facet	Hailang Dai Cheng Yin Xiaona Ye Bei Jiang Maowu Ran Zhuangqi Cao Xianfeng Chen
author_sort	Hailang Dai
title	A possible pathogenetic factor of sickle-cell disease based on fluorescent analysis via an optofluidic resonator
title_short	A possible pathogenetic factor of sickle-cell disease based on fluorescent analysis via an optofluidic resonator
title_full	A possible pathogenetic factor of sickle-cell disease based on fluorescent analysis via an optofluidic resonator
title_fullStr	A possible pathogenetic factor of sickle-cell disease based on fluorescent analysis via an optofluidic resonator
title_full_unstemmed	A possible pathogenetic factor of sickle-cell disease based on fluorescent analysis via an optofluidic resonator
title_sort	possible pathogenetic factor of sickle-cell disease based on fluorescent analysis via an optofluidic resonator
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6b11fb5d03de4e3d967475eb212f99bf
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A possible pathogenetic factor of sickle-cell disease based on fluorescent analysis via an optofluidic resonator

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