Detection of SARS-CoV-2 in wastewater is influenced by sampling time, concentration method, and target analyzed

The detection of SARS-CoV in wastewater has been proposed as a tool for monitoring COVID-19 at the community level. Although many reports have been published about detecting viral RNA in wastewater and its presence has been linked to infected people, appropriate analytical methodologies to use this...

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Autores principales:	Nancy J. Pino, Diana C. Rodriguez, Laura Castrillón Cano, Alejandra Rodriguez
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	covid-19 epidemiology method validation rt-pcr sars-cov-2 wastewater Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/6b1db59fcbab46588f2e33ea5df72947
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spelling	oai:doaj.org-article:6b1db59fcbab46588f2e33ea5df729472021-11-06T06:30:52ZDetection of SARS-CoV-2 in wastewater is influenced by sampling time, concentration method, and target analyzed1477-89201996-782910.2166/wh.2021.133https://doaj.org/article/6b1db59fcbab46588f2e33ea5df729472021-10-01T00:00:00Zhttp://jwh.iwaponline.com/content/19/5/775https://doaj.org/toc/1477-8920https://doaj.org/toc/1996-7829The detection of SARS-CoV in wastewater has been proposed as a tool for monitoring COVID-19 at the community level. Although many reports have been published about detecting viral RNA in wastewater and its presence has been linked to infected people, appropriate analytical methodologies to use this approach have not yet been established. In this study, we compared ultrafiltration, polyethylene glycol precipitation, flocculation using AlCl3, and flocculation with skim milk for the recovery of SARS-CoV-2, using RNA from patients with positive diagnoses for COVID-19 and Pseudomonas phage φ6 as the control. We also evaluated the primers for detecting the E, RdRp, and N genes of the virus, as well as different storage times. Differences in the recovery efficiencies were evident with the different concentration methods, the best being ultrafiltration and precipitation with aluminum, which had recovery rates of 42.0% and 30.0%, respectively, when virus was present at high levels. Significant differences were found between the recoveries using wastewater and deionized water and between different storage times, with better recoveries for 6 and 12 h samplings. The E gene was the only one detected in all the samples analyzed. The results show that although this approach can provide important data for studying the pandemic, clear protocols are necessary for investigations to be comparable. HIGHTLIGHTS RNA of SARS-CoV-2 was used to evaluate the recovery of different methods in wastewater.; The utility of phage φ6 of Pseudomonas as a control for evaluation of recovery methods of SARS-CoV-2 from wastewater was demonstrated.; Differences due to the target selected for the detection of SARS were demonstrated.; The results show that it is possible to detect SARS RNA in wastewater, however its epidemiological usefulness will depend on the correct validation of the technique used for detection.;Nancy J. PinoDiana C. RodriguezLaura Castrillón CanoAlejandra RodriguezIWA Publishingarticlecovid-19epidemiologymethod validationrt-pcrsars-cov-2wastewaterPublic aspects of medicineRA1-1270ENJournal of Water and Health, Vol 19, Iss 5, Pp 775-784 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	covid-19 epidemiology method validation rt-pcr sars-cov-2 wastewater Public aspects of medicine RA1-1270
spellingShingle	covid-19 epidemiology method validation rt-pcr sars-cov-2 wastewater Public aspects of medicine RA1-1270 Nancy J. Pino Diana C. Rodriguez Laura Castrillón Cano Alejandra Rodriguez Detection of SARS-CoV-2 in wastewater is influenced by sampling time, concentration method, and target analyzed
description	The detection of SARS-CoV in wastewater has been proposed as a tool for monitoring COVID-19 at the community level. Although many reports have been published about detecting viral RNA in wastewater and its presence has been linked to infected people, appropriate analytical methodologies to use this approach have not yet been established. In this study, we compared ultrafiltration, polyethylene glycol precipitation, flocculation using AlCl3, and flocculation with skim milk for the recovery of SARS-CoV-2, using RNA from patients with positive diagnoses for COVID-19 and Pseudomonas phage φ6 as the control. We also evaluated the primers for detecting the E, RdRp, and N genes of the virus, as well as different storage times. Differences in the recovery efficiencies were evident with the different concentration methods, the best being ultrafiltration and precipitation with aluminum, which had recovery rates of 42.0% and 30.0%, respectively, when virus was present at high levels. Significant differences were found between the recoveries using wastewater and deionized water and between different storage times, with better recoveries for 6 and 12 h samplings. The E gene was the only one detected in all the samples analyzed. The results show that although this approach can provide important data for studying the pandemic, clear protocols are necessary for investigations to be comparable. HIGHTLIGHTS RNA of SARS-CoV-2 was used to evaluate the recovery of different methods in wastewater.; The utility of phage φ6 of Pseudomonas as a control for evaluation of recovery methods of SARS-CoV-2 from wastewater was demonstrated.; Differences due to the target selected for the detection of SARS were demonstrated.; The results show that it is possible to detect SARS RNA in wastewater, however its epidemiological usefulness will depend on the correct validation of the technique used for detection.;
format	article
author	Nancy J. Pino Diana C. Rodriguez Laura Castrillón Cano Alejandra Rodriguez
author_facet	Nancy J. Pino Diana C. Rodriguez Laura Castrillón Cano Alejandra Rodriguez
author_sort	Nancy J. Pino
title	Detection of SARS-CoV-2 in wastewater is influenced by sampling time, concentration method, and target analyzed
title_short	Detection of SARS-CoV-2 in wastewater is influenced by sampling time, concentration method, and target analyzed
title_full	Detection of SARS-CoV-2 in wastewater is influenced by sampling time, concentration method, and target analyzed
title_fullStr	Detection of SARS-CoV-2 in wastewater is influenced by sampling time, concentration method, and target analyzed
title_full_unstemmed	Detection of SARS-CoV-2 in wastewater is influenced by sampling time, concentration method, and target analyzed
title_sort	detection of sars-cov-2 in wastewater is influenced by sampling time, concentration method, and target analyzed
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/6b1db59fcbab46588f2e33ea5df72947
work_keys_str_mv	AT nancyjpino detectionofsarscov2inwastewaterisinfluencedbysamplingtimeconcentrationmethodandtargetanalyzed AT dianacrodriguez detectionofsarscov2inwastewaterisinfluencedbysamplingtimeconcentrationmethodandtargetanalyzed AT lauracastrilloncano detectionofsarscov2inwastewaterisinfluencedbysamplingtimeconcentrationmethodandtargetanalyzed AT alejandrarodriguez detectionofsarscov2inwastewaterisinfluencedbysamplingtimeconcentrationmethodandtargetanalyzed
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Detection of SARS-CoV-2 in wastewater is influenced by sampling time, concentration method, and target analyzed

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