Bisdemethoxycurcumin Reduces Methicillin-Resistant <i>Staphylococcus aureus</i> Expression of Virulence-Related Exoproteins and Inhibits the Biofilm Formation

Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a major pathogen of nosocomial infection, which is resistant to most antibiotics. Presently, anti-virulence therapy and anti-biofilm therapy are considered to be promising alternatives. In the current work, we investigated the...

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Autores principales:	Shu Wang, Ok-Hwa Kang, Dong-Yeul Kwon
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	MRSA α-toxin enterotoxin A enterotoxin B biofilm bisdemethoxycurcumin Medicine R
Acceso en línea:	https://doaj.org/article/6b1f180ebb5e407f80485fc63310d35f
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spelling	oai:doaj.org-article:6b1f180ebb5e407f80485fc63310d35f2021-11-25T19:08:58ZBisdemethoxycurcumin Reduces Methicillin-Resistant <i>Staphylococcus aureus</i> Expression of Virulence-Related Exoproteins and Inhibits the Biofilm Formation10.3390/toxins131108042072-6651https://doaj.org/article/6b1f180ebb5e407f80485fc63310d35f2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/804https://doaj.org/toc/2072-6651Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a major pathogen of nosocomial infection, which is resistant to most antibiotics. Presently, anti-virulence therapy and anti-biofilm therapy are considered to be promising alternatives. In the current work, we investigated the influence of bisdemethoxycurcumin (BDMC) on the virulence-related exoproteins and the biofilm formation using a reference strain and clinic isolated strains. Western blotting, quantitative RT-PCR, and tumor necrosis factor (TNF) release assay were performed to assess the efficacy of BDMC in reducing the expression of <i>Staphylococcus</i> enterotoxin-related exoproteins (enterotoxin A, enterotoxin B) and α-toxin in MRSA. The anti-biofilm activity of BDMC was evaluated through a biofilm inhibition assay. The study suggests that sub-inhibitory concentrations of BDMC significantly inhibited the expression of <i>sea</i>, <i>seb</i>, and <i>hla</i> at the mRNA level in MRSA. Moreover, the expression of virulence-related exoproteins was significantly decreased by down-regulating accessory gene regulator <i>agr</i>, and the inhibition of biofilms formation was demonstrated by BDMC at sub-inhibitory concentrations. Consequently, the study suggests that BDMC may be a potential natural antibacterial agent to release the pressure brought by antibiotic resistance.Shu WangOk-Hwa KangDong-Yeul KwonMDPI AGarticleMRSAα-toxinenterotoxin Aenterotoxin BbiofilmbisdemethoxycurcuminMedicineRENToxins, Vol 13, Iss 804, p 804 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MRSA α-toxin enterotoxin A enterotoxin B biofilm bisdemethoxycurcumin Medicine R
spellingShingle	MRSA α-toxin enterotoxin A enterotoxin B biofilm bisdemethoxycurcumin Medicine R Shu Wang Ok-Hwa Kang Dong-Yeul Kwon Bisdemethoxycurcumin Reduces Methicillin-Resistant <i>Staphylococcus aureus</i> Expression of Virulence-Related Exoproteins and Inhibits the Biofilm Formation
description	Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a major pathogen of nosocomial infection, which is resistant to most antibiotics. Presently, anti-virulence therapy and anti-biofilm therapy are considered to be promising alternatives. In the current work, we investigated the influence of bisdemethoxycurcumin (BDMC) on the virulence-related exoproteins and the biofilm formation using a reference strain and clinic isolated strains. Western blotting, quantitative RT-PCR, and tumor necrosis factor (TNF) release assay were performed to assess the efficacy of BDMC in reducing the expression of <i>Staphylococcus</i> enterotoxin-related exoproteins (enterotoxin A, enterotoxin B) and α-toxin in MRSA. The anti-biofilm activity of BDMC was evaluated through a biofilm inhibition assay. The study suggests that sub-inhibitory concentrations of BDMC significantly inhibited the expression of <i>sea</i>, <i>seb</i>, and <i>hla</i> at the mRNA level in MRSA. Moreover, the expression of virulence-related exoproteins was significantly decreased by down-regulating accessory gene regulator <i>agr</i>, and the inhibition of biofilms formation was demonstrated by BDMC at sub-inhibitory concentrations. Consequently, the study suggests that BDMC may be a potential natural antibacterial agent to release the pressure brought by antibiotic resistance.
format	article
author	Shu Wang Ok-Hwa Kang Dong-Yeul Kwon
author_facet	Shu Wang Ok-Hwa Kang Dong-Yeul Kwon
author_sort	Shu Wang
title	Bisdemethoxycurcumin Reduces Methicillin-Resistant <i>Staphylococcus aureus</i> Expression of Virulence-Related Exoproteins and Inhibits the Biofilm Formation
title_short	Bisdemethoxycurcumin Reduces Methicillin-Resistant <i>Staphylococcus aureus</i> Expression of Virulence-Related Exoproteins and Inhibits the Biofilm Formation
title_full	Bisdemethoxycurcumin Reduces Methicillin-Resistant <i>Staphylococcus aureus</i> Expression of Virulence-Related Exoproteins and Inhibits the Biofilm Formation
title_fullStr	Bisdemethoxycurcumin Reduces Methicillin-Resistant <i>Staphylococcus aureus</i> Expression of Virulence-Related Exoproteins and Inhibits the Biofilm Formation
title_full_unstemmed	Bisdemethoxycurcumin Reduces Methicillin-Resistant <i>Staphylococcus aureus</i> Expression of Virulence-Related Exoproteins and Inhibits the Biofilm Formation
title_sort	bisdemethoxycurcumin reduces methicillin-resistant <i>staphylococcus aureus</i> expression of virulence-related exoproteins and inhibits the biofilm formation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6b1f180ebb5e407f80485fc63310d35f
work_keys_str_mv	AT shuwang bisdemethoxycurcuminreducesmethicillinresistantistaphylococcusaureusiexpressionofvirulencerelatedexoproteinsandinhibitsthebiofilmformation AT okhwakang bisdemethoxycurcuminreducesmethicillinresistantistaphylococcusaureusiexpressionofvirulencerelatedexoproteinsandinhibitsthebiofilmformation AT dongyeulkwon bisdemethoxycurcuminreducesmethicillinresistantistaphylococcusaureusiexpressionofvirulencerelatedexoproteinsandinhibitsthebiofilmformation
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Bisdemethoxycurcumin Reduces Methicillin-Resistant <i>Staphylococcus aureus</i> Expression of Virulence-Related Exoproteins and Inhibits the Biofilm Formation

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