Identification of biomarkers of human skin ageing in both genders. Wnt signalling - a label of skin ageing?

The goal of our work has been to investigate the mechanisms of gender-independent human skin ageing and examine the hypothesis of skin being an adequate model of global ageing. For this purpose, whole genome gene profiling was employed in sun-protected skin obtained from European Caucasian young and...

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Autores principales:	Evgenia Makrantonaki, Thore C Brink, Vasiliki Zampeli, Rana Mohsen Elewa, Barbara Mlody, Amir M Hossini, Bjoern Hermes, Ulf Krause, Juergen Knolle, Marwa Abdallah, James Adjaye, Christos C Zouboulis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6b33d3ff97ff4b76a949b0f25c86c956
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spelling	oai:doaj.org-article:6b33d3ff97ff4b76a949b0f25c86c9562021-11-18T08:06:52ZIdentification of biomarkers of human skin ageing in both genders. Wnt signalling - a label of skin ageing?1932-620310.1371/journal.pone.0050393https://doaj.org/article/6b33d3ff97ff4b76a949b0f25c86c9562012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23226273/?tool=EBIhttps://doaj.org/toc/1932-6203The goal of our work has been to investigate the mechanisms of gender-independent human skin ageing and examine the hypothesis of skin being an adequate model of global ageing. For this purpose, whole genome gene profiling was employed in sun-protected skin obtained from European Caucasian young and elderly females (mean age 26.7±4 years [n1 = 7] and 70.75±3.3 years [n2 = 4], respectively) and males (mean age 25.8±5.2 years [n3 = 6] and 76±3.8 years [n4 = 7], respectively) using the Illumina array platform. Confirmation of gene regulation was performed by real-time RT-PCR and immunohistochemistry. 523 genes were significantly regulated in female skin and 401 genes in male skin for the chosen criteria. Of these, 183 genes exhibited increased and 340 decreased expression in females whereas 210 genes showed increased and 191 decreased expression in males with age. In total, 39 genes were common in the target lists of significant regulated genes in males and females. 35 of these genes showed increased (16) or decreased (19) expression independent of gender. Only 4 overlapping genes (OR52N2, F6FR1OP2, TUBAL3 and STK40) showed differential regulation with age. Interestingly, Wnt signalling pathway showed to be significantly downregulated in aged skin with decreased gene and protein expression for males and females, accordingly. In addition, several genes involved in central nervous system (CNS) ageing (f.i. APP, TAU) showed to be expressed in human skin and were significanlty regulated with age. In conclusion, our study provides biomarkers of endogenous human skin ageing in both genders and highlight the role of Wnt signalling in this process. Furthermore, our data give evidence that skin could be used as a good alternative to understand ageing of different tissues such as CNS.Evgenia MakrantonakiThore C BrinkVasiliki ZampeliRana Mohsen ElewaBarbara MlodyAmir M HossiniBjoern HermesUlf KrauseJuergen KnolleMarwa AbdallahJames AdjayeChristos C ZouboulisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50393 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Evgenia Makrantonaki Thore C Brink Vasiliki Zampeli Rana Mohsen Elewa Barbara Mlody Amir M Hossini Bjoern Hermes Ulf Krause Juergen Knolle Marwa Abdallah James Adjaye Christos C Zouboulis Identification of biomarkers of human skin ageing in both genders. Wnt signalling - a label of skin ageing?
description	The goal of our work has been to investigate the mechanisms of gender-independent human skin ageing and examine the hypothesis of skin being an adequate model of global ageing. For this purpose, whole genome gene profiling was employed in sun-protected skin obtained from European Caucasian young and elderly females (mean age 26.7±4 years [n1 = 7] and 70.75±3.3 years [n2 = 4], respectively) and males (mean age 25.8±5.2 years [n3 = 6] and 76±3.8 years [n4 = 7], respectively) using the Illumina array platform. Confirmation of gene regulation was performed by real-time RT-PCR and immunohistochemistry. 523 genes were significantly regulated in female skin and 401 genes in male skin for the chosen criteria. Of these, 183 genes exhibited increased and 340 decreased expression in females whereas 210 genes showed increased and 191 decreased expression in males with age. In total, 39 genes were common in the target lists of significant regulated genes in males and females. 35 of these genes showed increased (16) or decreased (19) expression independent of gender. Only 4 overlapping genes (OR52N2, F6FR1OP2, TUBAL3 and STK40) showed differential regulation with age. Interestingly, Wnt signalling pathway showed to be significantly downregulated in aged skin with decreased gene and protein expression for males and females, accordingly. In addition, several genes involved in central nervous system (CNS) ageing (f.i. APP, TAU) showed to be expressed in human skin and were significanlty regulated with age. In conclusion, our study provides biomarkers of endogenous human skin ageing in both genders and highlight the role of Wnt signalling in this process. Furthermore, our data give evidence that skin could be used as a good alternative to understand ageing of different tissues such as CNS.
format	article
author	Evgenia Makrantonaki Thore C Brink Vasiliki Zampeli Rana Mohsen Elewa Barbara Mlody Amir M Hossini Bjoern Hermes Ulf Krause Juergen Knolle Marwa Abdallah James Adjaye Christos C Zouboulis
author_facet	Evgenia Makrantonaki Thore C Brink Vasiliki Zampeli Rana Mohsen Elewa Barbara Mlody Amir M Hossini Bjoern Hermes Ulf Krause Juergen Knolle Marwa Abdallah James Adjaye Christos C Zouboulis
author_sort	Evgenia Makrantonaki
title	Identification of biomarkers of human skin ageing in both genders. Wnt signalling - a label of skin ageing?
title_short	Identification of biomarkers of human skin ageing in both genders. Wnt signalling - a label of skin ageing?
title_full	Identification of biomarkers of human skin ageing in both genders. Wnt signalling - a label of skin ageing?
title_fullStr	Identification of biomarkers of human skin ageing in both genders. Wnt signalling - a label of skin ageing?
title_full_unstemmed	Identification of biomarkers of human skin ageing in both genders. Wnt signalling - a label of skin ageing?
title_sort	identification of biomarkers of human skin ageing in both genders. wnt signalling - a label of skin ageing?
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6b33d3ff97ff4b76a949b0f25c86c956
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Identification of biomarkers of human skin ageing in both genders. Wnt signalling - a label of skin ageing?

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