Longitudinal profiling of the burn patient cutaneous and gastrointestinal microbiota: a pilot study

Abstract Sepsis is a leading cause of morbidity and mortality in patients that have sustained a severe burn injury. Early detection and treatment of infections improves outcomes and understanding changes in the host microbiome following injury and during treatment may aid in burn care. The loss of f...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kelly M. Lima, Ryan R. Davis, Stephenie Y. Liu, David G. Greenhalgh, Nam K. Tran
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6b34a577643848ff915cacfa4e6cdbe4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6b34a577643848ff915cacfa4e6cdbe4
record_format	dspace
spelling	oai:doaj.org-article:6b34a577643848ff915cacfa4e6cdbe42021-12-02T16:49:37ZLongitudinal profiling of the burn patient cutaneous and gastrointestinal microbiota: a pilot study10.1038/s41598-021-89822-z2045-2322https://doaj.org/article/6b34a577643848ff915cacfa4e6cdbe42021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89822-zhttps://doaj.org/toc/2045-2322Abstract Sepsis is a leading cause of morbidity and mortality in patients that have sustained a severe burn injury. Early detection and treatment of infections improves outcomes and understanding changes in the host microbiome following injury and during treatment may aid in burn care. The loss of functional barriers, systemic inflammation, and commensal community perturbations all contribute to a burn patient’s increased risk of infection. We sampled 10 burn patients to evaluate cutaneous microbial populations on the burn wound and corresponding spared skin on days 0, 3, 7, 14, 21, and 28 post-intensive care unit admission. In addition, skin samples were paired with perianal and rectal locations to evaluate changes in the burn patient gut microbiome following injury and treatment. We found significant (P = 0.011) reduction in alpha diversity on the burn wound compared to spared skin throughout the sampling period as well as reduction in common skin commensal bacteria such as Propionibacterium acnes and Staphylococcus epidermitis. Compared to healthy volunteers (n = 18), the burn patient spared skin also exhibited a significant reduction in alpha diversity (P = 0.001). Treatments such as systemic or topical antibiotic administration, skin grafting, and nutritional formulations also impact diversity and community composition at the sampling locations. When evaluating each subject individually, an increase in relative abundance of taxa isolated clinically by bacterial culture could be seen in 5/9 infections detected among the burn patient cohort.Kelly M. LimaRyan R. DavisStephenie Y. LiuDavid G. GreenhalghNam K. TranNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kelly M. Lima Ryan R. Davis Stephenie Y. Liu David G. Greenhalgh Nam K. Tran Longitudinal profiling of the burn patient cutaneous and gastrointestinal microbiota: a pilot study
description	Abstract Sepsis is a leading cause of morbidity and mortality in patients that have sustained a severe burn injury. Early detection and treatment of infections improves outcomes and understanding changes in the host microbiome following injury and during treatment may aid in burn care. The loss of functional barriers, systemic inflammation, and commensal community perturbations all contribute to a burn patient’s increased risk of infection. We sampled 10 burn patients to evaluate cutaneous microbial populations on the burn wound and corresponding spared skin on days 0, 3, 7, 14, 21, and 28 post-intensive care unit admission. In addition, skin samples were paired with perianal and rectal locations to evaluate changes in the burn patient gut microbiome following injury and treatment. We found significant (P = 0.011) reduction in alpha diversity on the burn wound compared to spared skin throughout the sampling period as well as reduction in common skin commensal bacteria such as Propionibacterium acnes and Staphylococcus epidermitis. Compared to healthy volunteers (n = 18), the burn patient spared skin also exhibited a significant reduction in alpha diversity (P = 0.001). Treatments such as systemic or topical antibiotic administration, skin grafting, and nutritional formulations also impact diversity and community composition at the sampling locations. When evaluating each subject individually, an increase in relative abundance of taxa isolated clinically by bacterial culture could be seen in 5/9 infections detected among the burn patient cohort.
format	article
author	Kelly M. Lima Ryan R. Davis Stephenie Y. Liu David G. Greenhalgh Nam K. Tran
author_facet	Kelly M. Lima Ryan R. Davis Stephenie Y. Liu David G. Greenhalgh Nam K. Tran
author_sort	Kelly M. Lima
title	Longitudinal profiling of the burn patient cutaneous and gastrointestinal microbiota: a pilot study
title_short	Longitudinal profiling of the burn patient cutaneous and gastrointestinal microbiota: a pilot study
title_full	Longitudinal profiling of the burn patient cutaneous and gastrointestinal microbiota: a pilot study
title_fullStr	Longitudinal profiling of the burn patient cutaneous and gastrointestinal microbiota: a pilot study
title_full_unstemmed	Longitudinal profiling of the burn patient cutaneous and gastrointestinal microbiota: a pilot study
title_sort	longitudinal profiling of the burn patient cutaneous and gastrointestinal microbiota: a pilot study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6b34a577643848ff915cacfa4e6cdbe4
work_keys_str_mv	AT kellymlima longitudinalprofilingoftheburnpatientcutaneousandgastrointestinalmicrobiotaapilotstudy AT ryanrdavis longitudinalprofilingoftheburnpatientcutaneousandgastrointestinalmicrobiotaapilotstudy AT stephenieyliu longitudinalprofilingoftheburnpatientcutaneousandgastrointestinalmicrobiotaapilotstudy AT davidggreenhalgh longitudinalprofilingoftheburnpatientcutaneousandgastrointestinalmicrobiotaapilotstudy AT namktran longitudinalprofilingoftheburnpatientcutaneousandgastrointestinalmicrobiotaapilotstudy
_version_	1718383282656116736

Longitudinal profiling of the burn patient cutaneous and gastrointestinal microbiota: a pilot study

Ejemplares similares