Patterned immobilization of antibodies within roll-to-roll hot embossed polymeric microfluidic channels.

This paper describes a method for the patterned immobilization of capture antibodies into a microfluidic platform fabricated by roll-to-roll (R2R) hot embossing on poly (methyl methacrylate) (PMMA). Covalent attachment of antibodies was achieved by two sequential inkjet printing steps. First, a poly...

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Autores principales:	Belachew Feyssa, Christina Liedert, Liisa Kivimaki, Leena-Sisko Johansson, Heli Jantunen, Leena Hakalahti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6b3d9d5eeb53440ab3ea03385beec699
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spelling	oai:doaj.org-article:6b3d9d5eeb53440ab3ea03385beec6992021-11-18T07:37:16ZPatterned immobilization of antibodies within roll-to-roll hot embossed polymeric microfluidic channels.1932-620310.1371/journal.pone.0068918https://doaj.org/article/6b3d9d5eeb53440ab3ea03385beec6992013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874811/?tool=EBIhttps://doaj.org/toc/1932-6203This paper describes a method for the patterned immobilization of capture antibodies into a microfluidic platform fabricated by roll-to-roll (R2R) hot embossing on poly (methyl methacrylate) (PMMA). Covalent attachment of antibodies was achieved by two sequential inkjet printing steps. First, a polyethyleneimine (PEI) layer was deposited onto oxygen plasma activated PMMA foil and further cross-linked with glutaraldehyde (GA) to provide an amine-reactive aldehyde surface (PEI-GA). This step was followed by a second deposition of antibody by overprinting on the PEI-GA patterned PMMA foil. The PEI polymer ink was first formulated to ensure stable drop formation in inkjet printing and the printed films were characterized using atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). Anti-CRP antibody was patterned on PMMA foil by the developed method and bonded permanently with R2R hot embossed PMMA microchannels by solvent bonding lamination. The functionality of the immobilized antibody inside the microfluidic channel was evaluated by fluorescence-based sandwich immunoassay for detection of C-reactive protein (CRP). The antibody-antigen assay exhibited a good level of linearity over the range of 10 ng/ml to 500 ng/ml (R(2) = 0.991) with a calculated detection limit of 5.2 ng/ml. The developed patterning method is straightforward, rapid and provides a versatile approach for creating multiple protein patterns in a single microfluidic channel for multiplexed immunoassays.Belachew FeyssaChristina LiedertLiisa KivimakiLeena-Sisko JohanssonHeli JantunenLeena HakalahtiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68918 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Belachew Feyssa Christina Liedert Liisa Kivimaki Leena-Sisko Johansson Heli Jantunen Leena Hakalahti Patterned immobilization of antibodies within roll-to-roll hot embossed polymeric microfluidic channels.
description	This paper describes a method for the patterned immobilization of capture antibodies into a microfluidic platform fabricated by roll-to-roll (R2R) hot embossing on poly (methyl methacrylate) (PMMA). Covalent attachment of antibodies was achieved by two sequential inkjet printing steps. First, a polyethyleneimine (PEI) layer was deposited onto oxygen plasma activated PMMA foil and further cross-linked with glutaraldehyde (GA) to provide an amine-reactive aldehyde surface (PEI-GA). This step was followed by a second deposition of antibody by overprinting on the PEI-GA patterned PMMA foil. The PEI polymer ink was first formulated to ensure stable drop formation in inkjet printing and the printed films were characterized using atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). Anti-CRP antibody was patterned on PMMA foil by the developed method and bonded permanently with R2R hot embossed PMMA microchannels by solvent bonding lamination. The functionality of the immobilized antibody inside the microfluidic channel was evaluated by fluorescence-based sandwich immunoassay for detection of C-reactive protein (CRP). The antibody-antigen assay exhibited a good level of linearity over the range of 10 ng/ml to 500 ng/ml (R(2) = 0.991) with a calculated detection limit of 5.2 ng/ml. The developed patterning method is straightforward, rapid and provides a versatile approach for creating multiple protein patterns in a single microfluidic channel for multiplexed immunoassays.
format	article
author	Belachew Feyssa Christina Liedert Liisa Kivimaki Leena-Sisko Johansson Heli Jantunen Leena Hakalahti
author_facet	Belachew Feyssa Christina Liedert Liisa Kivimaki Leena-Sisko Johansson Heli Jantunen Leena Hakalahti
author_sort	Belachew Feyssa
title	Patterned immobilization of antibodies within roll-to-roll hot embossed polymeric microfluidic channels.
title_short	Patterned immobilization of antibodies within roll-to-roll hot embossed polymeric microfluidic channels.
title_full	Patterned immobilization of antibodies within roll-to-roll hot embossed polymeric microfluidic channels.
title_fullStr	Patterned immobilization of antibodies within roll-to-roll hot embossed polymeric microfluidic channels.
title_full_unstemmed	Patterned immobilization of antibodies within roll-to-roll hot embossed polymeric microfluidic channels.
title_sort	patterned immobilization of antibodies within roll-to-roll hot embossed polymeric microfluidic channels.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6b3d9d5eeb53440ab3ea03385beec699
work_keys_str_mv	AT belachewfeyssa patternedimmobilizationofantibodieswithinrolltorollhotembossedpolymericmicrofluidicchannels AT christinaliedert patternedimmobilizationofantibodieswithinrolltorollhotembossedpolymericmicrofluidicchannels AT liisakivimaki patternedimmobilizationofantibodieswithinrolltorollhotembossedpolymericmicrofluidicchannels AT leenasiskojohansson patternedimmobilizationofantibodieswithinrolltorollhotembossedpolymericmicrofluidicchannels AT helijantunen patternedimmobilizationofantibodieswithinrolltorollhotembossedpolymericmicrofluidicchannels AT leenahakalahti patternedimmobilizationofantibodieswithinrolltorollhotembossedpolymericmicrofluidicchannels
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Patterned immobilization of antibodies within roll-to-roll hot embossed polymeric microfluidic channels.

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