A genetic screen for Drosophila social isolation mutants and analysis of sex pistol

Abstract Prolonged periods of forced social isolation is detrimental to well-being, yet we know little about which genes regulate susceptibility to its effects. In the fruit fly, Drosophila melanogaster, social isolation induces stark changes in behavior including increased aggression, locomotor act...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Mark Eddison
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6b3f1c3d63d74e239faf919eb1f3d536
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6b3f1c3d63d74e239faf919eb1f3d536
record_format	dspace
spelling	oai:doaj.org-article:6b3f1c3d63d74e239faf919eb1f3d5362021-12-02T16:38:49ZA genetic screen for Drosophila social isolation mutants and analysis of sex pistol10.1038/s41598-021-96871-x2045-2322https://doaj.org/article/6b3f1c3d63d74e239faf919eb1f3d5362021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96871-xhttps://doaj.org/toc/2045-2322Abstract Prolonged periods of forced social isolation is detrimental to well-being, yet we know little about which genes regulate susceptibility to its effects. In the fruit fly, Drosophila melanogaster, social isolation induces stark changes in behavior including increased aggression, locomotor activity, and resistance to ethanol sedation. To identify genes regulating sensitivity to isolation, I screened a collection of sixteen hundred P-element insertion lines for mutants with abnormal levels of all three isolation-induced behaviors. The screen identified three mutants whose affected genes are likely central to regulating the effects of isolation in flies. One mutant, sex pistol (sxp), became extremely aggressive and resistant to ethanol sedation when socially isolated. sxp also had a high level of male–male courtship. The mutation in sxp reduced the expression of two minor isoforms of the actin regulator hts (adducin), as well as mildly reducing expression of CalpA, a calcium-dependent protease. As a consequence, sxp also had increased expression of the insulin-like peptide, dILP5. Analysis of the social behavior of sxp suggests that these minor hts isoforms function to limit isolation-induced aggression, while chronically high levels of dILP5 increase male–male courtship.Mark EddisonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mark Eddison A genetic screen for Drosophila social isolation mutants and analysis of sex pistol
description	Abstract Prolonged periods of forced social isolation is detrimental to well-being, yet we know little about which genes regulate susceptibility to its effects. In the fruit fly, Drosophila melanogaster, social isolation induces stark changes in behavior including increased aggression, locomotor activity, and resistance to ethanol sedation. To identify genes regulating sensitivity to isolation, I screened a collection of sixteen hundred P-element insertion lines for mutants with abnormal levels of all three isolation-induced behaviors. The screen identified three mutants whose affected genes are likely central to regulating the effects of isolation in flies. One mutant, sex pistol (sxp), became extremely aggressive and resistant to ethanol sedation when socially isolated. sxp also had a high level of male–male courtship. The mutation in sxp reduced the expression of two minor isoforms of the actin regulator hts (adducin), as well as mildly reducing expression of CalpA, a calcium-dependent protease. As a consequence, sxp also had increased expression of the insulin-like peptide, dILP5. Analysis of the social behavior of sxp suggests that these minor hts isoforms function to limit isolation-induced aggression, while chronically high levels of dILP5 increase male–male courtship.
format	article
author	Mark Eddison
author_facet	Mark Eddison
author_sort	Mark Eddison
title	A genetic screen for Drosophila social isolation mutants and analysis of sex pistol
title_short	A genetic screen for Drosophila social isolation mutants and analysis of sex pistol
title_full	A genetic screen for Drosophila social isolation mutants and analysis of sex pistol
title_fullStr	A genetic screen for Drosophila social isolation mutants and analysis of sex pistol
title_full_unstemmed	A genetic screen for Drosophila social isolation mutants and analysis of sex pistol
title_sort	genetic screen for drosophila social isolation mutants and analysis of sex pistol
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6b3f1c3d63d74e239faf919eb1f3d536
work_keys_str_mv	AT markeddison ageneticscreenfordrosophilasocialisolationmutantsandanalysisofsexpistol AT markeddison geneticscreenfordrosophilasocialisolationmutantsandanalysisofsexpistol
_version_	1718383578352451584

A genetic screen for Drosophila social isolation mutants and analysis of sex pistol

Ejemplares similares