Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets

Poly ADP-ribosylation (PARylation) is a highly dynamic post-translation protein modification, but most methods only detect stable PARylation events. Here the authors develop a split-GFP-based sensor for PARylation detection in live cells and use it to identify a new centrosomal PARylation target.

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Detalles Bibliográficos
Autores principales:	Dragomir B. Krastev, Stephen J. Pettitt, James Campbell, Feifei Song, Barbara E. Tanos, Stoyno S. Stoynov, Alan Ashworth, Christopher J. Lord
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6b4485c994204db1bfb5e33371cc705b
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spelling	oai:doaj.org-article:6b4485c994204db1bfb5e33371cc705b2021-12-02T17:33:18ZCoupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets10.1038/s41467-018-04466-42041-1723https://doaj.org/article/6b4485c994204db1bfb5e33371cc705b2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04466-4https://doaj.org/toc/2041-1723Poly ADP-ribosylation (PARylation) is a highly dynamic post-translation protein modification, but most methods only detect stable PARylation events. Here the authors develop a split-GFP-based sensor for PARylation detection in live cells and use it to identify a new centrosomal PARylation target.Dragomir B. KrastevStephen J. PettittJames CampbellFeifei SongBarbara E. TanosStoyno S. StoynovAlan AshworthChristopher J. LordNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Dragomir B. Krastev Stephen J. Pettitt James Campbell Feifei Song Barbara E. Tanos Stoyno S. Stoynov Alan Ashworth Christopher J. Lord Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets
description	Poly ADP-ribosylation (PARylation) is a highly dynamic post-translation protein modification, but most methods only detect stable PARylation events. Here the authors develop a split-GFP-based sensor for PARylation detection in live cells and use it to identify a new centrosomal PARylation target.
format	article
author	Dragomir B. Krastev Stephen J. Pettitt James Campbell Feifei Song Barbara E. Tanos Stoyno S. Stoynov Alan Ashworth Christopher J. Lord
author_facet	Dragomir B. Krastev Stephen J. Pettitt James Campbell Feifei Song Barbara E. Tanos Stoyno S. Stoynov Alan Ashworth Christopher J. Lord
author_sort	Dragomir B. Krastev
title	Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets
title_short	Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets
title_full	Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets
title_fullStr	Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets
title_full_unstemmed	Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets
title_sort	coupling bimolecular parylation biosensors with genetic screens to identify parylation targets
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6b4485c994204db1bfb5e33371cc705b
work_keys_str_mv	AT dragomirbkrastev couplingbimolecularparylationbiosensorswithgeneticscreenstoidentifyparylationtargets AT stephenjpettitt couplingbimolecularparylationbiosensorswithgeneticscreenstoidentifyparylationtargets AT jamescampbell couplingbimolecularparylationbiosensorswithgeneticscreenstoidentifyparylationtargets AT feifeisong couplingbimolecularparylationbiosensorswithgeneticscreenstoidentifyparylationtargets AT barbaraetanos couplingbimolecularparylationbiosensorswithgeneticscreenstoidentifyparylationtargets AT stoynosstoynov couplingbimolecularparylationbiosensorswithgeneticscreenstoidentifyparylationtargets AT alanashworth couplingbimolecularparylationbiosensorswithgeneticscreenstoidentifyparylationtargets AT christopherjlord couplingbimolecularparylationbiosensorswithgeneticscreenstoidentifyparylationtargets
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Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets

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