Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets
Poly ADP-ribosylation (PARylation) is a highly dynamic post-translation protein modification, but most methods only detect stable PARylation events. Here the authors develop a split-GFP-based sensor for PARylation detection in live cells and use it to identify a new centrosomal PARylation target.
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | article |
| Language: | EN |
| Published: |
Nature Portfolio
2018
|
| Subjects: | |
| Online Access: | https://doaj.org/article/6b4485c994204db1bfb5e33371cc705b |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|