Development of molecular resources for the Chinese horseshoe crab Tachypleus tridentatus

The Chinese horseshoe crab Tachypleus tridentatus is an endangered marine benthic species of great biomedical value and scientific significance. In this study, T. tridentatus hemocyte transcriptome was sequenced to rapidly develop molecular resources using the Illumina paired-end sequencing platform...

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Autores principales:	H Li, Y Xia, Y Rao, S Liu, J Zhang, L Cai
Formato:	article
Lenguaje:	EN
Publicado:	Inter-Research 2015
Materias:	Biology (General) QH301-705.5 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6b4baa755370466d83f13729380fb0f8
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spelling	oai:doaj.org-article:6b4baa755370466d83f13729380fb0f82021-11-18T09:16:50ZDevelopment of molecular resources for the Chinese horseshoe crab Tachypleus tridentatus1864-77821864-779010.3354/ab00632https://doaj.org/article/6b4baa755370466d83f13729380fb0f82015-05-01T00:00:00Zhttps://www.int-res.com/abstracts/ab/v24/n1/p17-24/https://doaj.org/toc/1864-7782https://doaj.org/toc/1864-7790The Chinese horseshoe crab Tachypleus tridentatus is an endangered marine benthic species of great biomedical value and scientific significance. In this study, T. tridentatus hemocyte transcriptome was sequenced to rapidly develop molecular resources using the Illumina paired-end sequencing platform. Deep sequencing generated a total of 38402764 reads that consisted of 7.7 Gbp raw data, which were assembled into 38759 unigenes with an average length of 668.6 bp. A total of 608 microsatellite loci and 71959 high-confidence single nucleotide polymorphisms were discovered. All the assembled unigenes were annotated by running BLASTx similarity searches on Nr, Swiss-Prot, COG, and KEGG databases; of which 17446 (45.0%) unigenes showed significant matches (E-value <1e-5) to known sequences in public databases. As determined through gene ontology annotation and KEGG pathway mapping, the functional annotation of the unigenes signified diverse biological functions and processes. Transcripts potentially involved in immune defense were identified among these genes. These transcripts included pattern recognition receptors, stress response genes, complement components, and immune effectors. This study provides important insights into the transcript sequences of T. tridentatus, which may facilitate studies on the conservation genetics and development of biomedical applications for T. tridentatus.H LiY XiaY RaoS LiuJ ZhangL CaiInter-ResearcharticleBiology (General)QH301-705.5MicrobiologyQR1-502ENAquatic Biology, Vol 24, Iss 1, Pp 17-24 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5 Microbiology QR1-502
spellingShingle	Biology (General) QH301-705.5 Microbiology QR1-502 H Li Y Xia Y Rao S Liu J Zhang L Cai Development of molecular resources for the Chinese horseshoe crab Tachypleus tridentatus
description	The Chinese horseshoe crab Tachypleus tridentatus is an endangered marine benthic species of great biomedical value and scientific significance. In this study, T. tridentatus hemocyte transcriptome was sequenced to rapidly develop molecular resources using the Illumina paired-end sequencing platform. Deep sequencing generated a total of 38402764 reads that consisted of 7.7 Gbp raw data, which were assembled into 38759 unigenes with an average length of 668.6 bp. A total of 608 microsatellite loci and 71959 high-confidence single nucleotide polymorphisms were discovered. All the assembled unigenes were annotated by running BLASTx similarity searches on Nr, Swiss-Prot, COG, and KEGG databases; of which 17446 (45.0%) unigenes showed significant matches (E-value <1e-5) to known sequences in public databases. As determined through gene ontology annotation and KEGG pathway mapping, the functional annotation of the unigenes signified diverse biological functions and processes. Transcripts potentially involved in immune defense were identified among these genes. These transcripts included pattern recognition receptors, stress response genes, complement components, and immune effectors. This study provides important insights into the transcript sequences of T. tridentatus, which may facilitate studies on the conservation genetics and development of biomedical applications for T. tridentatus.
format	article
author	H Li Y Xia Y Rao S Liu J Zhang L Cai
author_facet	H Li Y Xia Y Rao S Liu J Zhang L Cai
author_sort	H Li
title	Development of molecular resources for the Chinese horseshoe crab Tachypleus tridentatus
title_short	Development of molecular resources for the Chinese horseshoe crab Tachypleus tridentatus
title_full	Development of molecular resources for the Chinese horseshoe crab Tachypleus tridentatus
title_fullStr	Development of molecular resources for the Chinese horseshoe crab Tachypleus tridentatus
title_full_unstemmed	Development of molecular resources for the Chinese horseshoe crab Tachypleus tridentatus
title_sort	development of molecular resources for the chinese horseshoe crab tachypleus tridentatus
publisher	Inter-Research
publishDate	2015
url	https://doaj.org/article/6b4baa755370466d83f13729380fb0f8
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_version_	1718420906696507392

Development of molecular resources for the Chinese horseshoe crab Tachypleus tridentatus

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