Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis

Abstract Cytomegalovirus (CMV) reactivation in the colon is common in patients with severe ulcerative colitis (UC). Ganciclovir (GCV) resistance conferring CMV UL97 gene mutations have been reported in recent years. However, the prevalence of UL97 gene mutations in GCV-naive CMV infection in the col...

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Autores principales:	Satoshi Tamura, Satoshi Osawa, Natsuki Ishida, Takahiro Miyazu, Shinya Tani, Mihoko Yamade, Moriya Iwaizumi, Yasushi Hamaya, Isao Kosugi, Takahisa Furuta, Ken Sugimoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6b4d912ca3a04a1d8a4240916d4ef469
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spelling	oai:doaj.org-article:6b4d912ca3a04a1d8a4240916d4ef4692021-12-02T16:32:12ZPrevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis10.1038/s41598-021-93168-x2045-2322https://doaj.org/article/6b4d912ca3a04a1d8a4240916d4ef4692021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93168-xhttps://doaj.org/toc/2045-2322Abstract Cytomegalovirus (CMV) reactivation in the colon is common in patients with severe ulcerative colitis (UC). Ganciclovir (GCV) resistance conferring CMV UL97 gene mutations have been reported in recent years. However, the prevalence of UL97 gene mutations in GCV-naive CMV infection in the colon remains unknown. We investigated the prevalence of CMV UL97 gene mutations in patients with colonic CMV infection associated with or without UC. Twenty-two GCV-naive patients with colonic CMV infection, 15 with UC and 7 with other diseases, were enrolled. Frozen biopsy samples or formalin-fixed paraffin-embedded samples were used for nested polymerase chain reaction (PCR) amplification of the UL97 gene. Sanger DNA sequencing was performed. In comparison with AD169 reference strain, natural polymorphisms were frequently detected in codons N68D (100%), I244V (100%), and D605E (86.4%). Seven polymorphisms were detected infrequently (< 10%) outside the kinase domain. However, no known GCV resistance mutations were found. There seemed to be no difference between the ratio of polymorphisms in patients with and without UC. In conclusions, we did not detect UL97 gene mutations associated with GCV resistance in GCV-naive patients with or without UC. Consistent with previous reports, D605E polymorphism may be used as a genetic marker for CMV in East Asian countries.Satoshi TamuraSatoshi OsawaNatsuki IshidaTakahiro MiyazuShinya TaniMihoko YamadeMoriya IwaizumiYasushi HamayaIsao KosugiTakahisa FurutaKen SugimotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Satoshi Tamura Satoshi Osawa Natsuki Ishida Takahiro Miyazu Shinya Tani Mihoko Yamade Moriya Iwaizumi Yasushi Hamaya Isao Kosugi Takahisa Furuta Ken Sugimoto Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis
description	Abstract Cytomegalovirus (CMV) reactivation in the colon is common in patients with severe ulcerative colitis (UC). Ganciclovir (GCV) resistance conferring CMV UL97 gene mutations have been reported in recent years. However, the prevalence of UL97 gene mutations in GCV-naive CMV infection in the colon remains unknown. We investigated the prevalence of CMV UL97 gene mutations in patients with colonic CMV infection associated with or without UC. Twenty-two GCV-naive patients with colonic CMV infection, 15 with UC and 7 with other diseases, were enrolled. Frozen biopsy samples or formalin-fixed paraffin-embedded samples were used for nested polymerase chain reaction (PCR) amplification of the UL97 gene. Sanger DNA sequencing was performed. In comparison with AD169 reference strain, natural polymorphisms were frequently detected in codons N68D (100%), I244V (100%), and D605E (86.4%). Seven polymorphisms were detected infrequently (< 10%) outside the kinase domain. However, no known GCV resistance mutations were found. There seemed to be no difference between the ratio of polymorphisms in patients with and without UC. In conclusions, we did not detect UL97 gene mutations associated with GCV resistance in GCV-naive patients with or without UC. Consistent with previous reports, D605E polymorphism may be used as a genetic marker for CMV in East Asian countries.
format	article
author	Satoshi Tamura Satoshi Osawa Natsuki Ishida Takahiro Miyazu Shinya Tani Mihoko Yamade Moriya Iwaizumi Yasushi Hamaya Isao Kosugi Takahisa Furuta Ken Sugimoto
author_facet	Satoshi Tamura Satoshi Osawa Natsuki Ishida Takahiro Miyazu Shinya Tani Mihoko Yamade Moriya Iwaizumi Yasushi Hamaya Isao Kosugi Takahisa Furuta Ken Sugimoto
author_sort	Satoshi Tamura
title	Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis
title_short	Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis
title_full	Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis
title_fullStr	Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis
title_full_unstemmed	Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis
title_sort	prevalence of ul97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6b4d912ca3a04a1d8a4240916d4ef469
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Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis

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