Effects of Caryota mitis profilin-loaded PLGA nanoparticles in a murine model of allergic asthma

Xiaojun Xiao,1,* Xiaowei Zeng,2,* Xinxin Zhang,3,* Li Ma,3 Xiaoyu Liu,1 Haiqiong Yu,1 Lin Mei,2 Zhigang Liu1 1Institute of Allergy and Immunology, School of Medicine, Shenzhen University, Shenzhen, 2Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 3Fa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiao X, Zeng X, Zhang X, Ma L, Liu X, Yu H, Mei L, Liu Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6b500565d7354ac4b565f462bbab87ce
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6b500565d7354ac4b565f462bbab87ce
record_format	dspace
spelling	oai:doaj.org-article:6b500565d7354ac4b565f462bbab87ce2021-12-02T00:53:29ZEffects of Caryota mitis profilin-loaded PLGA nanoparticles in a murine model of allergic asthma1176-91141178-2013https://doaj.org/article/6b500565d7354ac4b565f462bbab87ce2013-11-01T00:00:00Zhttp://www.dovepress.com/effects-of-caryota-mitis-profilin-loaded-plga-nanoparticles-in-a-murin-a15078https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Xiaojun Xiao,1,* Xiaowei Zeng,2,* Xinxin Zhang,3,* Li Ma,3 Xiaoyu Liu,1 Haiqiong Yu,1 Lin Mei,2 Zhigang Liu1 1Institute of Allergy and Immunology, School of Medicine, Shenzhen University, Shenzhen, 2Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 3Faculty of Basic Medical Science, Nanchang University, Nanchang, People's Republic of China *These authors contributed equally to this work Background: Pollen allergy is the most common allergic disease. However, tropical pollens, such as those of Palmae, have seldom been investigated compared with the specific immunotherapy studies done on hyperallergenic birch, olive, and ragweed pollens. Although poly(lactic-co-glycolic acid) (PLGA) has been extensively applied as a biodegradable polymer in medical devices, it has rarely been utilized as a vaccine adjuvant to prevent and treat allergic disease. In this study, we investigated the immunotherapeutic effects of recombinant Caryota mitis profilin (rCmP)-loaded PLGA nanoparticles and the underlying mechanisms involved. Methods: A mouse model of allergenic asthma was established for specific immunotherapy using rCmP-loaded PLGA nanoparticles as the adjuvant. The model was evaluated by determining airway hyperresponsiveness and levels of serum-specific antibodies (IgE, IgG, and IgG2a) and cytokines, and observing histologic sections of lung tissue. Results: The rCmP-loaded PLGA nanoparticles effectively inhibited generation of specific IgE and secretion of the Th2 cytokine interleukin-4, facilitated generation of specific IgG2a and secretion of the Th1 cytokine interferon-gamma, converted the Th2 response to Th1, and evidently alleviated allergic symptoms. Conclusion: PLGA functions more appropriately as a specific immunotherapy adjuvant for allergen vaccines than does conventional Al(OH)3 due to its superior efficacy, longer potency, and markedly fewer side effects. The rCmP-loaded PLGA nanoparticles developed herein offer a promising avenue for specific immunotherapy in allergic asthma. Keywords: nanoparticles, Caryota mitis profilin, PLGA, allergic asthma, adjuvantXiao XZeng XZhang XMa LLiu XYu HMei LLiu ZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 4553-4562 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Xiao X Zeng X Zhang X Ma L Liu X Yu H Mei L Liu Z Effects of Caryota mitis profilin-loaded PLGA nanoparticles in a murine model of allergic asthma
description	Xiaojun Xiao,1,* Xiaowei Zeng,2,* Xinxin Zhang,3,* Li Ma,3 Xiaoyu Liu,1 Haiqiong Yu,1 Lin Mei,2 Zhigang Liu1 1Institute of Allergy and Immunology, School of Medicine, Shenzhen University, Shenzhen, 2Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 3Faculty of Basic Medical Science, Nanchang University, Nanchang, People's Republic of China *These authors contributed equally to this work Background: Pollen allergy is the most common allergic disease. However, tropical pollens, such as those of Palmae, have seldom been investigated compared with the specific immunotherapy studies done on hyperallergenic birch, olive, and ragweed pollens. Although poly(lactic-co-glycolic acid) (PLGA) has been extensively applied as a biodegradable polymer in medical devices, it has rarely been utilized as a vaccine adjuvant to prevent and treat allergic disease. In this study, we investigated the immunotherapeutic effects of recombinant Caryota mitis profilin (rCmP)-loaded PLGA nanoparticles and the underlying mechanisms involved. Methods: A mouse model of allergenic asthma was established for specific immunotherapy using rCmP-loaded PLGA nanoparticles as the adjuvant. The model was evaluated by determining airway hyperresponsiveness and levels of serum-specific antibodies (IgE, IgG, and IgG2a) and cytokines, and observing histologic sections of lung tissue. Results: The rCmP-loaded PLGA nanoparticles effectively inhibited generation of specific IgE and secretion of the Th2 cytokine interleukin-4, facilitated generation of specific IgG2a and secretion of the Th1 cytokine interferon-gamma, converted the Th2 response to Th1, and evidently alleviated allergic symptoms. Conclusion: PLGA functions more appropriately as a specific immunotherapy adjuvant for allergen vaccines than does conventional Al(OH)3 due to its superior efficacy, longer potency, and markedly fewer side effects. The rCmP-loaded PLGA nanoparticles developed herein offer a promising avenue for specific immunotherapy in allergic asthma. Keywords: nanoparticles, Caryota mitis profilin, PLGA, allergic asthma, adjuvant
format	article
author	Xiao X Zeng X Zhang X Ma L Liu X Yu H Mei L Liu Z
author_facet	Xiao X Zeng X Zhang X Ma L Liu X Yu H Mei L Liu Z
author_sort	Xiao X
title	Effects of Caryota mitis profilin-loaded PLGA nanoparticles in a murine model of allergic asthma
title_short	Effects of Caryota mitis profilin-loaded PLGA nanoparticles in a murine model of allergic asthma
title_full	Effects of Caryota mitis profilin-loaded PLGA nanoparticles in a murine model of allergic asthma
title_fullStr	Effects of Caryota mitis profilin-loaded PLGA nanoparticles in a murine model of allergic asthma
title_full_unstemmed	Effects of Caryota mitis profilin-loaded PLGA nanoparticles in a murine model of allergic asthma
title_sort	effects of caryota mitis profilin-loaded plga nanoparticles in a murine model of allergic asthma
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/6b500565d7354ac4b565f462bbab87ce
work_keys_str_mv	AT xiaox effectsofcaryotamitisprofilinloadedplgananoparticlesinamurinemodelofallergicasthma AT zengx effectsofcaryotamitisprofilinloadedplgananoparticlesinamurinemodelofallergicasthma AT zhangx effectsofcaryotamitisprofilinloadedplgananoparticlesinamurinemodelofallergicasthma AT mal effectsofcaryotamitisprofilinloadedplgananoparticlesinamurinemodelofallergicasthma AT liux effectsofcaryotamitisprofilinloadedplgananoparticlesinamurinemodelofallergicasthma AT yuh effectsofcaryotamitisprofilinloadedplgananoparticlesinamurinemodelofallergicasthma AT meil effectsofcaryotamitisprofilinloadedplgananoparticlesinamurinemodelofallergicasthma AT liuz effectsofcaryotamitisprofilinloadedplgananoparticlesinamurinemodelofallergicasthma
_version_	1718403444022181888

Effects of Caryota mitis profilin-loaded PLGA nanoparticles in a murine model of allergic asthma

Ejemplares similares