Overexpression of miR-340 inhibits cell proliferation and induces apoptosis of human bladder cancer via targeting Glut-1

Abstract Background Bladder cancer (BC) has high mortality due to distant metastasis. Previous works suggested that microRNA (miRNA)-340 is a critical regulator for the development and progression of various cancers. The specific biological function of miR-340 in BC is little known. Methods In the p...

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Autores principales:	Gang Xu, Shouhua Pan, Zhirong Zhu, Junlong Li
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Bladder cancer miR-340 Glut-1 Proliferation Apoptosis Diseases of the genitourinary system. Urology RC870-923
Acceso en línea:	https://doaj.org/article/6b59fc2fbfa0474dbefd063c71c12eb3
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spelling	oai:doaj.org-article:6b59fc2fbfa0474dbefd063c71c12eb32021-12-05T12:19:53ZOverexpression of miR-340 inhibits cell proliferation and induces apoptosis of human bladder cancer via targeting Glut-110.1186/s12894-021-00935-z1471-2490https://doaj.org/article/6b59fc2fbfa0474dbefd063c71c12eb32021-12-01T00:00:00Zhttps://doi.org/10.1186/s12894-021-00935-zhttps://doaj.org/toc/1471-2490Abstract Background Bladder cancer (BC) has high mortality due to distant metastasis. Previous works suggested that microRNA (miRNA)-340 is a critical regulator for the development and progression of various cancers. The specific biological function of miR-340 in BC is little known. Methods In the present study, RT-qPCR was performed to measure the expression of miR-340 in paired BC tissues and adjacent non-tumor tissues. Next, the target gene of miR-340 was identified using dual-luciferase reporter assay and its level was also tested in tissues. Moreover, cell proliferation and apoptosis were analyzed by CCK-8 and flow cytometry. Finally, the expression of PCNA, Bax was detected by RT-qPCR and western blotting, as well as PI3K/AKT signaling measured by western blotting. Result The results demonstrated that miR-340 expression was downregulated and its target Glut-1 level was upregulated in BC tissues. Functionally, overexpression of miR-340 suppressed the proliferation and induced apoptosis in BC cells, while Glut-1 reversed the suppression of proliferation or induction of apoptosis induced by miR-340. Additionally, miR-340 repressed PCNA, p-PI3K and p-AKT levels but enhanced Bax level, while Glut-1 rescued the effects. Conclusion In conclusion, miR-340 functions as a tumor suppressor of BC, which inhibited proliferation and induced apoptosis by targeting Glut-1 partly through regulating PCNA, Bax expression and PI3K/AKT pathway. This study suggested that miR-340 is a potential target for the treatment of BC.Gang XuShouhua PanZhirong ZhuJunlong LiBMCarticleBladder cancermiR-340Glut-1ProliferationApoptosisDiseases of the genitourinary system. UrologyRC870-923ENBMC Urology, Vol 21, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bladder cancer miR-340 Glut-1 Proliferation Apoptosis Diseases of the genitourinary system. Urology RC870-923
spellingShingle	Bladder cancer miR-340 Glut-1 Proliferation Apoptosis Diseases of the genitourinary system. Urology RC870-923 Gang Xu Shouhua Pan Zhirong Zhu Junlong Li Overexpression of miR-340 inhibits cell proliferation and induces apoptosis of human bladder cancer via targeting Glut-1
description	Abstract Background Bladder cancer (BC) has high mortality due to distant metastasis. Previous works suggested that microRNA (miRNA)-340 is a critical regulator for the development and progression of various cancers. The specific biological function of miR-340 in BC is little known. Methods In the present study, RT-qPCR was performed to measure the expression of miR-340 in paired BC tissues and adjacent non-tumor tissues. Next, the target gene of miR-340 was identified using dual-luciferase reporter assay and its level was also tested in tissues. Moreover, cell proliferation and apoptosis were analyzed by CCK-8 and flow cytometry. Finally, the expression of PCNA, Bax was detected by RT-qPCR and western blotting, as well as PI3K/AKT signaling measured by western blotting. Result The results demonstrated that miR-340 expression was downregulated and its target Glut-1 level was upregulated in BC tissues. Functionally, overexpression of miR-340 suppressed the proliferation and induced apoptosis in BC cells, while Glut-1 reversed the suppression of proliferation or induction of apoptosis induced by miR-340. Additionally, miR-340 repressed PCNA, p-PI3K and p-AKT levels but enhanced Bax level, while Glut-1 rescued the effects. Conclusion In conclusion, miR-340 functions as a tumor suppressor of BC, which inhibited proliferation and induced apoptosis by targeting Glut-1 partly through regulating PCNA, Bax expression and PI3K/AKT pathway. This study suggested that miR-340 is a potential target for the treatment of BC.
format	article
author	Gang Xu Shouhua Pan Zhirong Zhu Junlong Li
author_facet	Gang Xu Shouhua Pan Zhirong Zhu Junlong Li
author_sort	Gang Xu
title	Overexpression of miR-340 inhibits cell proliferation and induces apoptosis of human bladder cancer via targeting Glut-1
title_short	Overexpression of miR-340 inhibits cell proliferation and induces apoptosis of human bladder cancer via targeting Glut-1
title_full	Overexpression of miR-340 inhibits cell proliferation and induces apoptosis of human bladder cancer via targeting Glut-1
title_fullStr	Overexpression of miR-340 inhibits cell proliferation and induces apoptosis of human bladder cancer via targeting Glut-1
title_full_unstemmed	Overexpression of miR-340 inhibits cell proliferation and induces apoptosis of human bladder cancer via targeting Glut-1
title_sort	overexpression of mir-340 inhibits cell proliferation and induces apoptosis of human bladder cancer via targeting glut-1
publisher	BMC
publishDate	2021
url	https://doaj.org/article/6b59fc2fbfa0474dbefd063c71c12eb3
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Overexpression of miR-340 inhibits cell proliferation and induces apoptosis of human bladder cancer via targeting Glut-1

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