Hypothalamic effects of progesterone on regulation of the pulsatile and surge release of luteinising hormone in female rats

Abstract Progesterone can block the oestradiol-induced GnRH/LH surge and inhibit LH pulse frequency. Recent studies reported that progesterone prevented premature LH surges during ovarian hyperstimulation in women. As the most potent stimulator of GnRH/LH release, kisspeptin is believed to mediate t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wen He, Xiaofeng Li, Daniel Adekunbi, Yali Liu, Hui Long, Li Wang, Qifeng Lyu, Yanping Kuang, Kevin T. O’Byrne
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6b5a3e6ae15043359e0a6294b127a08b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6b5a3e6ae15043359e0a6294b127a08b
record_format	dspace
spelling	oai:doaj.org-article:6b5a3e6ae15043359e0a6294b127a08b2021-12-02T12:32:16ZHypothalamic effects of progesterone on regulation of the pulsatile and surge release of luteinising hormone in female rats10.1038/s41598-017-08805-12045-2322https://doaj.org/article/6b5a3e6ae15043359e0a6294b127a08b2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08805-1https://doaj.org/toc/2045-2322Abstract Progesterone can block the oestradiol-induced GnRH/LH surge and inhibit LH pulse frequency. Recent studies reported that progesterone prevented premature LH surges during ovarian hyperstimulation in women. As the most potent stimulator of GnRH/LH release, kisspeptin is believed to mediate the positive and negative feedback effects of oestradiol in the hypothalamic anteroventral periventricular (AVPV) and arcuate (ARC) nuclei, while the region-specific role of progesterone receptors in these nuclei remains unknown. This study examined the hypothesis that progesterone inhibits LH surge and pulsatile secretion via its receptor in the ARC and/or AVPV nuclei. Adult female rats received a single injection of pregnant mare serum gonadotropin followed by progesterone or vehicle. Progesterone administration resulted in a significant prolongation of the oestrous cycle and blockade of LH surge. However, microinjection of the progesterone receptor antagonist, RU486, into the AVPV reversed the prolonged cycle length and rescued the progesterone blockade LH surge, while RU486 into the ARC shortened LH pulse interval in the progesterone treated rats. These results demonstrated that progesterone’s inhibitory effect on the GnRH/LH surge and pulsatile secretion is mediated by its receptor in the kisspeptin enriched hypothalamic AVPV and ARC respectively, which are essential for progesterone regulation of oestrous cyclicity in rats.Wen HeXiaofeng LiDaniel AdekunbiYali LiuHui LongLi WangQifeng LyuYanping KuangKevin T. O’ByrneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wen He Xiaofeng Li Daniel Adekunbi Yali Liu Hui Long Li Wang Qifeng Lyu Yanping Kuang Kevin T. O’Byrne Hypothalamic effects of progesterone on regulation of the pulsatile and surge release of luteinising hormone in female rats
description	Abstract Progesterone can block the oestradiol-induced GnRH/LH surge and inhibit LH pulse frequency. Recent studies reported that progesterone prevented premature LH surges during ovarian hyperstimulation in women. As the most potent stimulator of GnRH/LH release, kisspeptin is believed to mediate the positive and negative feedback effects of oestradiol in the hypothalamic anteroventral periventricular (AVPV) and arcuate (ARC) nuclei, while the region-specific role of progesterone receptors in these nuclei remains unknown. This study examined the hypothesis that progesterone inhibits LH surge and pulsatile secretion via its receptor in the ARC and/or AVPV nuclei. Adult female rats received a single injection of pregnant mare serum gonadotropin followed by progesterone or vehicle. Progesterone administration resulted in a significant prolongation of the oestrous cycle and blockade of LH surge. However, microinjection of the progesterone receptor antagonist, RU486, into the AVPV reversed the prolonged cycle length and rescued the progesterone blockade LH surge, while RU486 into the ARC shortened LH pulse interval in the progesterone treated rats. These results demonstrated that progesterone’s inhibitory effect on the GnRH/LH surge and pulsatile secretion is mediated by its receptor in the kisspeptin enriched hypothalamic AVPV and ARC respectively, which are essential for progesterone regulation of oestrous cyclicity in rats.
format	article
author	Wen He Xiaofeng Li Daniel Adekunbi Yali Liu Hui Long Li Wang Qifeng Lyu Yanping Kuang Kevin T. O’Byrne
author_facet	Wen He Xiaofeng Li Daniel Adekunbi Yali Liu Hui Long Li Wang Qifeng Lyu Yanping Kuang Kevin T. O’Byrne
author_sort	Wen He
title	Hypothalamic effects of progesterone on regulation of the pulsatile and surge release of luteinising hormone in female rats
title_short	Hypothalamic effects of progesterone on regulation of the pulsatile and surge release of luteinising hormone in female rats
title_full	Hypothalamic effects of progesterone on regulation of the pulsatile and surge release of luteinising hormone in female rats
title_fullStr	Hypothalamic effects of progesterone on regulation of the pulsatile and surge release of luteinising hormone in female rats
title_full_unstemmed	Hypothalamic effects of progesterone on regulation of the pulsatile and surge release of luteinising hormone in female rats
title_sort	hypothalamic effects of progesterone on regulation of the pulsatile and surge release of luteinising hormone in female rats
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6b5a3e6ae15043359e0a6294b127a08b
work_keys_str_mv	AT wenhe hypothalamiceffectsofprogesteroneonregulationofthepulsatileandsurgereleaseofluteinisinghormoneinfemalerats AT xiaofengli hypothalamiceffectsofprogesteroneonregulationofthepulsatileandsurgereleaseofluteinisinghormoneinfemalerats AT danieladekunbi hypothalamiceffectsofprogesteroneonregulationofthepulsatileandsurgereleaseofluteinisinghormoneinfemalerats AT yaliliu hypothalamiceffectsofprogesteroneonregulationofthepulsatileandsurgereleaseofluteinisinghormoneinfemalerats AT huilong hypothalamiceffectsofprogesteroneonregulationofthepulsatileandsurgereleaseofluteinisinghormoneinfemalerats AT liwang hypothalamiceffectsofprogesteroneonregulationofthepulsatileandsurgereleaseofluteinisinghormoneinfemalerats AT qifenglyu hypothalamiceffectsofprogesteroneonregulationofthepulsatileandsurgereleaseofluteinisinghormoneinfemalerats AT yanpingkuang hypothalamiceffectsofprogesteroneonregulationofthepulsatileandsurgereleaseofluteinisinghormoneinfemalerats AT kevintobyrne hypothalamiceffectsofprogesteroneonregulationofthepulsatileandsurgereleaseofluteinisinghormoneinfemalerats
_version_	1718394099051003904

Hypothalamic effects of progesterone on regulation of the pulsatile and surge release of luteinising hormone in female rats

Ejemplares similares