Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding

Abstract In mammals, the intestine harbors numerous bacteria that play an important role in health. Intestinal microbiota have also been thought to be an important factor in the health of shrimp. However, the barrier systems of the digestive tracts of shrimp seem to be different from those of mammal...

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Autores principales:	Kentaro Imaizumi, Sasiwipa Tinwongger, Hidehiro Kondo, Ikuo Hirono
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6b5ed8e4dcfb4b48b59f5215a78c39d0
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spelling	oai:doaj.org-article:6b5ed8e4dcfb4b48b59f5215a78c39d02021-12-02T17:02:21ZAnalysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding10.1038/s41598-021-89415-w2045-2322https://doaj.org/article/6b5ed8e4dcfb4b48b59f5215a78c39d02021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89415-whttps://doaj.org/toc/2045-2322Abstract In mammals, the intestine harbors numerous bacteria that play an important role in health. Intestinal microbiota have also been thought to be an important factor in the health of shrimp. However, the barrier systems of the digestive tracts of shrimp seem to be different from those of mammals. In this study, we analyzed the bacterial composition in the stomach and midgut of two species of shrimp during administration of a probiotic, Bacillus amyloliquefaciens strain TOA5001 by analysis of 16S rRNA genes with Illumina sequencing technology. Whiteleg shrimp Litopenaeus vannamei were observed under laboratory conditions and kuruma shrimp Marsupenaeus japonicus were observed in an aquaculture farm. The diversities of bacteria in the stomachs of both shrimps were significantly higher than those in the midgut. Also, the microbiota changed during probiotic feeding. Feeding whiteleg shrimp the probiotic after being challenged with an acute hepatopancreatic necrosis disease (AHPND)-causing strain of Vibrio parahaemolyticus increased their survival compared to the control group, which suggested that the probiotic prevented AHPND. These results appear to show that a probiotic can affect the microbiota throughout digestive tract of penaeid shrimps and that probiotic can have a role in preventing disease.Kentaro ImaizumiSasiwipa TinwonggerHidehiro KondoIkuo HironoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kentaro Imaizumi Sasiwipa Tinwongger Hidehiro Kondo Ikuo Hirono Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding
description	Abstract In mammals, the intestine harbors numerous bacteria that play an important role in health. Intestinal microbiota have also been thought to be an important factor in the health of shrimp. However, the barrier systems of the digestive tracts of shrimp seem to be different from those of mammals. In this study, we analyzed the bacterial composition in the stomach and midgut of two species of shrimp during administration of a probiotic, Bacillus amyloliquefaciens strain TOA5001 by analysis of 16S rRNA genes with Illumina sequencing technology. Whiteleg shrimp Litopenaeus vannamei were observed under laboratory conditions and kuruma shrimp Marsupenaeus japonicus were observed in an aquaculture farm. The diversities of bacteria in the stomachs of both shrimps were significantly higher than those in the midgut. Also, the microbiota changed during probiotic feeding. Feeding whiteleg shrimp the probiotic after being challenged with an acute hepatopancreatic necrosis disease (AHPND)-causing strain of Vibrio parahaemolyticus increased their survival compared to the control group, which suggested that the probiotic prevented AHPND. These results appear to show that a probiotic can affect the microbiota throughout digestive tract of penaeid shrimps and that probiotic can have a role in preventing disease.
format	article
author	Kentaro Imaizumi Sasiwipa Tinwongger Hidehiro Kondo Ikuo Hirono
author_facet	Kentaro Imaizumi Sasiwipa Tinwongger Hidehiro Kondo Ikuo Hirono
author_sort	Kentaro Imaizumi
title	Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding
title_short	Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding
title_full	Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding
title_fullStr	Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding
title_full_unstemmed	Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding
title_sort	analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6b5ed8e4dcfb4b48b59f5215a78c39d0
work_keys_str_mv	AT kentaroimaizumi analysisofmicrobiotainthestomachandmidgutoftwopenaeidshrimpsduringprobioticfeeding AT sasiwipatinwongger analysisofmicrobiotainthestomachandmidgutoftwopenaeidshrimpsduringprobioticfeeding AT hidehirokondo analysisofmicrobiotainthestomachandmidgutoftwopenaeidshrimpsduringprobioticfeeding AT ikuohirono analysisofmicrobiotainthestomachandmidgutoftwopenaeidshrimpsduringprobioticfeeding
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Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding

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