Increased expression of TAZ and associated upregulation of PD-L1 in cervical cancer

Abstract Background As an important component of the Hippo pathway, WW domain-containing transcription regulator 1 (TAZ), is a transcriptional coactivator that is responsible for the progression of various types of cancers. Programmed cell death protein 1 (PD-1) receptors in activated T cells and th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yanyan Han, Dandan Liu, Lianhong Li
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	TAZ PD-L1 Cervical cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/6b5ef1ffbff846899812b06570569846
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6b5ef1ffbff846899812b06570569846
record_format	dspace
spelling	oai:doaj.org-article:6b5ef1ffbff846899812b065705698462021-11-08T11:13:34ZIncreased expression of TAZ and associated upregulation of PD-L1 in cervical cancer10.1186/s12935-021-02287-y1475-2867https://doaj.org/article/6b5ef1ffbff846899812b065705698462021-11-01T00:00:00Zhttps://doi.org/10.1186/s12935-021-02287-yhttps://doaj.org/toc/1475-2867Abstract Background As an important component of the Hippo pathway, WW domain-containing transcription regulator 1 (TAZ), is a transcriptional coactivator that is responsible for the progression of various types of cancers. Programmed cell death protein 1 (PD-1) receptors in activated T cells and their ligand programming death force 1 (PD-L1) are the main checkpoint signals that control T cell activity. Studies have shown high levels of PD-L1 in various cancers and that PD-L1/PD-1 signals to evade T-cell immunity. Recent data have demonstrated that TAZ can regulate the characteristics of cancer cells via PD-L1. Cervical cancer is a common gynecological disease worldwide. In this study, we attempted to evaluate the effects of TAZ and PD-L1 on cervical cancer. Methods Hela cervical cancer cells were transfected with TAZ plasmid or TAZ siRNA or PD-L1 siRNA by using Lipofectamine 2000. The relationship between TAZ and PD-L1 in cervical cancer cells was determined by qRT-PCR and western blotting. The functional roles of TAZ were confirmed via CCK-8, Transwell and flow cytometry assays. Western blotting was utilized to observe the expression of BCL-2 and Caspase-3. The clinicopathological correlation of TAZ and PD-L1 was evaluated via relevant databases. Result TAZ is upregulated in cervical cancer and induces the growth and metastasis of cervical cancer cells by targeting PD-L1and inhibiting the ratio of apoptotic of cancer cells. High TAZ and PD-L1 expression was observed in different stage, grade, histological patterns, and ages of cervical cancer groups compared with normal cervix groups. Furthermore, high TAZ expression was positively correlated with the infiltration levels of immune cells and the expression of PD-L1.Yanyan HanDandan LiuLianhong LiBMCarticleTAZPD-L1Cervical cancerNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282CytologyQH573-671ENCancer Cell International, Vol 21, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	TAZ PD-L1 Cervical cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
spellingShingle	TAZ PD-L1 Cervical cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671 Yanyan Han Dandan Liu Lianhong Li Increased expression of TAZ and associated upregulation of PD-L1 in cervical cancer
description	Abstract Background As an important component of the Hippo pathway, WW domain-containing transcription regulator 1 (TAZ), is a transcriptional coactivator that is responsible for the progression of various types of cancers. Programmed cell death protein 1 (PD-1) receptors in activated T cells and their ligand programming death force 1 (PD-L1) are the main checkpoint signals that control T cell activity. Studies have shown high levels of PD-L1 in various cancers and that PD-L1/PD-1 signals to evade T-cell immunity. Recent data have demonstrated that TAZ can regulate the characteristics of cancer cells via PD-L1. Cervical cancer is a common gynecological disease worldwide. In this study, we attempted to evaluate the effects of TAZ and PD-L1 on cervical cancer. Methods Hela cervical cancer cells were transfected with TAZ plasmid or TAZ siRNA or PD-L1 siRNA by using Lipofectamine 2000. The relationship between TAZ and PD-L1 in cervical cancer cells was determined by qRT-PCR and western blotting. The functional roles of TAZ were confirmed via CCK-8, Transwell and flow cytometry assays. Western blotting was utilized to observe the expression of BCL-2 and Caspase-3. The clinicopathological correlation of TAZ and PD-L1 was evaluated via relevant databases. Result TAZ is upregulated in cervical cancer and induces the growth and metastasis of cervical cancer cells by targeting PD-L1and inhibiting the ratio of apoptotic of cancer cells. High TAZ and PD-L1 expression was observed in different stage, grade, histological patterns, and ages of cervical cancer groups compared with normal cervix groups. Furthermore, high TAZ expression was positively correlated with the infiltration levels of immune cells and the expression of PD-L1.
format	article
author	Yanyan Han Dandan Liu Lianhong Li
author_facet	Yanyan Han Dandan Liu Lianhong Li
author_sort	Yanyan Han
title	Increased expression of TAZ and associated upregulation of PD-L1 in cervical cancer
title_short	Increased expression of TAZ and associated upregulation of PD-L1 in cervical cancer
title_full	Increased expression of TAZ and associated upregulation of PD-L1 in cervical cancer
title_fullStr	Increased expression of TAZ and associated upregulation of PD-L1 in cervical cancer
title_full_unstemmed	Increased expression of TAZ and associated upregulation of PD-L1 in cervical cancer
title_sort	increased expression of taz and associated upregulation of pd-l1 in cervical cancer
publisher	BMC
publishDate	2021
url	https://doaj.org/article/6b5ef1ffbff846899812b06570569846
work_keys_str_mv	AT yanyanhan increasedexpressionoftazandassociatedupregulationofpdl1incervicalcancer AT dandanliu increasedexpressionoftazandassociatedupregulationofpdl1incervicalcancer AT lianhongli increasedexpressionoftazandassociatedupregulationofpdl1incervicalcancer
_version_	1718442342446268416

Increased expression of TAZ and associated upregulation of PD-L1 in cervical cancer

Ejemplares similares