CTmax is repeatable and doesn’t reduce growth in zebrafish

Abstract Critical thermal maximum (CTmax) is a commonly and increasingly used measure of an animal’s upper thermal tolerance limit. However, it is unknown how consistent CTmax is within an individual, and how physiologically taxing such experiments are. We addressed this by estimating the repeatabil...

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Autores principales:	Rachael Morgan, Mette H. Finnøen, Fredrik Jutfelt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6b6a4c4b77a64ec097a25fdb81be60b9
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spelling	oai:doaj.org-article:6b6a4c4b77a64ec097a25fdb81be60b92021-12-02T16:08:15ZCTmax is repeatable and doesn’t reduce growth in zebrafish10.1038/s41598-018-25593-42045-2322https://doaj.org/article/6b6a4c4b77a64ec097a25fdb81be60b92018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25593-4https://doaj.org/toc/2045-2322Abstract Critical thermal maximum (CTmax) is a commonly and increasingly used measure of an animal’s upper thermal tolerance limit. However, it is unknown how consistent CTmax is within an individual, and how physiologically taxing such experiments are. We addressed this by estimating the repeatability of CTmax in zebrafish, and measured how growth and survival were affected by multiple trials. The repeatability of CTmax over four trials was 0.22 (0.07–0.43). However, CTmax increased from the first to the second trial, likely because of thermal acclimation triggered by the heat shock. After this initial acclimation response individuals became more consistent in their CTmax, reflected in a higher repeatability measure of 0.45 (0.28–0.65) for trials 2–4. We found a high innate thermal tolerance led to a lower acclimation response, whereas a high acclimation response was present in individuals that displayed a low initial CTmax. This could indicate that different strategies for thermal tolerance (i.e. plasticity vs. high innate tolerance) can co-exist in a population. Additionally, repeated CTmax trials had no effect on growth, and survival was high (99%). This validates the method and, combined with the relatively high repeatability, highlights the relevance of CTmax for continued use as a metric for acute thermal tolerance.Rachael MorganMette H. FinnøenFredrik JutfeltNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rachael Morgan Mette H. Finnøen Fredrik Jutfelt CTmax is repeatable and doesn’t reduce growth in zebrafish
description	Abstract Critical thermal maximum (CTmax) is a commonly and increasingly used measure of an animal’s upper thermal tolerance limit. However, it is unknown how consistent CTmax is within an individual, and how physiologically taxing such experiments are. We addressed this by estimating the repeatability of CTmax in zebrafish, and measured how growth and survival were affected by multiple trials. The repeatability of CTmax over four trials was 0.22 (0.07–0.43). However, CTmax increased from the first to the second trial, likely because of thermal acclimation triggered by the heat shock. After this initial acclimation response individuals became more consistent in their CTmax, reflected in a higher repeatability measure of 0.45 (0.28–0.65) for trials 2–4. We found a high innate thermal tolerance led to a lower acclimation response, whereas a high acclimation response was present in individuals that displayed a low initial CTmax. This could indicate that different strategies for thermal tolerance (i.e. plasticity vs. high innate tolerance) can co-exist in a population. Additionally, repeated CTmax trials had no effect on growth, and survival was high (99%). This validates the method and, combined with the relatively high repeatability, highlights the relevance of CTmax for continued use as a metric for acute thermal tolerance.
format	article
author	Rachael Morgan Mette H. Finnøen Fredrik Jutfelt
author_facet	Rachael Morgan Mette H. Finnøen Fredrik Jutfelt
author_sort	Rachael Morgan
title	CTmax is repeatable and doesn’t reduce growth in zebrafish
title_short	CTmax is repeatable and doesn’t reduce growth in zebrafish
title_full	CTmax is repeatable and doesn’t reduce growth in zebrafish
title_fullStr	CTmax is repeatable and doesn’t reduce growth in zebrafish
title_full_unstemmed	CTmax is repeatable and doesn’t reduce growth in zebrafish
title_sort	ctmax is repeatable and doesn’t reduce growth in zebrafish
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6b6a4c4b77a64ec097a25fdb81be60b9
work_keys_str_mv	AT rachaelmorgan ctmaxisrepeatableanddoesntreducegrowthinzebrafish AT mettehfinnøen ctmaxisrepeatableanddoesntreducegrowthinzebrafish AT fredrikjutfelt ctmaxisrepeatableanddoesntreducegrowthinzebrafish
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CTmax is repeatable and doesn’t reduce growth in zebrafish

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