The Transcription Factor VdHapX Controls Iron Homeostasis and Is Crucial for Virulence in the Vascular Pathogen <named-content content-type="genus-species">Verticillium dahliae</named-content>

ABSTRACT Iron homeostasis is essential for full virulence and viability in many pathogenic fungi. Here, we showed that the bZip transcription factor VdHapX functions as a key regulator of iron homeostasis for adaptation to iron-depleted and iron-excess conditions and is required for full virulence i...

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Autores principales:	Yonglin Wang, Chenglin Deng, Longyan Tian, Dianguang Xiong, Chengming Tian, Steven J. Klosterman
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	HapX Verticillium dahliae fungal virulence iron homeostasis vascular wilt Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6b6f872451774b16b28f52e8060fa788
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spelling	oai:doaj.org-article:6b6f872451774b16b28f52e8060fa7882021-11-15T15:22:26ZThe Transcription Factor VdHapX Controls Iron Homeostasis and Is Crucial for Virulence in the Vascular Pathogen <named-content content-type="genus-species">Verticillium dahliae</named-content>10.1128/mSphere.00400-182379-5042https://doaj.org/article/6b6f872451774b16b28f52e8060fa7882018-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00400-18https://doaj.org/toc/2379-5042ABSTRACT Iron homeostasis is essential for full virulence and viability in many pathogenic fungi. Here, we showed that the bZip transcription factor VdHapX functions as a key regulator of iron homeostasis for adaptation to iron-depleted and iron-excess conditions and is required for full virulence in the vascular wilt fungus, Verticillium dahliae. Deletion of VdHapX impaired mycelial growth and conidiation under both iron starvation and iron sufficiency. Furthermore, disruption of VdHapX led to decreased formation of the long-lived survival structures of V. dahliae, known as microsclerotia. Expression of genes involved in iron utilization pathways and siderophore biosynthesis was misregulated in the ΔVdHapX strain under the iron-depleted condition. Additionally, the ΔVdHapX strain exhibited increased sensitivity to high iron concentrations and H2O2, indicating that VdHapX also contributes to iron or H2O2 detoxification. The ΔVdHapX strain showed a strong reduction in virulence on smoke tree seedlings (Cotinus coggygria) and was delayed in its ability to penetrate plant epidermal tissue. IMPORTANCE This study demonstrated that VdHapX is a conserved protein that mediates adaptation to iron starvation and excesses, affects microsclerotium formation, and is crucial for virulence of V. dahliae.Yonglin WangChenglin DengLongyan TianDianguang XiongChengming TianSteven J. KlostermanAmerican Society for MicrobiologyarticleHapXVerticillium dahliaefungal virulenceiron homeostasisvascular wiltMicrobiologyQR1-502ENmSphere, Vol 3, Iss 5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	HapX Verticillium dahliae fungal virulence iron homeostasis vascular wilt Microbiology QR1-502
spellingShingle	HapX Verticillium dahliae fungal virulence iron homeostasis vascular wilt Microbiology QR1-502 Yonglin Wang Chenglin Deng Longyan Tian Dianguang Xiong Chengming Tian Steven J. Klosterman The Transcription Factor VdHapX Controls Iron Homeostasis and Is Crucial for Virulence in the Vascular Pathogen <named-content content-type="genus-species">Verticillium dahliae</named-content>
description	ABSTRACT Iron homeostasis is essential for full virulence and viability in many pathogenic fungi. Here, we showed that the bZip transcription factor VdHapX functions as a key regulator of iron homeostasis for adaptation to iron-depleted and iron-excess conditions and is required for full virulence in the vascular wilt fungus, Verticillium dahliae. Deletion of VdHapX impaired mycelial growth and conidiation under both iron starvation and iron sufficiency. Furthermore, disruption of VdHapX led to decreased formation of the long-lived survival structures of V. dahliae, known as microsclerotia. Expression of genes involved in iron utilization pathways and siderophore biosynthesis was misregulated in the ΔVdHapX strain under the iron-depleted condition. Additionally, the ΔVdHapX strain exhibited increased sensitivity to high iron concentrations and H2O2, indicating that VdHapX also contributes to iron or H2O2 detoxification. The ΔVdHapX strain showed a strong reduction in virulence on smoke tree seedlings (Cotinus coggygria) and was delayed in its ability to penetrate plant epidermal tissue. IMPORTANCE This study demonstrated that VdHapX is a conserved protein that mediates adaptation to iron starvation and excesses, affects microsclerotium formation, and is crucial for virulence of V. dahliae.
format	article
author	Yonglin Wang Chenglin Deng Longyan Tian Dianguang Xiong Chengming Tian Steven J. Klosterman
author_facet	Yonglin Wang Chenglin Deng Longyan Tian Dianguang Xiong Chengming Tian Steven J. Klosterman
author_sort	Yonglin Wang
title	The Transcription Factor VdHapX Controls Iron Homeostasis and Is Crucial for Virulence in the Vascular Pathogen <named-content content-type="genus-species">Verticillium dahliae</named-content>
title_short	The Transcription Factor VdHapX Controls Iron Homeostasis and Is Crucial for Virulence in the Vascular Pathogen <named-content content-type="genus-species">Verticillium dahliae</named-content>
title_full	The Transcription Factor VdHapX Controls Iron Homeostasis and Is Crucial for Virulence in the Vascular Pathogen <named-content content-type="genus-species">Verticillium dahliae</named-content>
title_fullStr	The Transcription Factor VdHapX Controls Iron Homeostasis and Is Crucial for Virulence in the Vascular Pathogen <named-content content-type="genus-species">Verticillium dahliae</named-content>
title_full_unstemmed	The Transcription Factor VdHapX Controls Iron Homeostasis and Is Crucial for Virulence in the Vascular Pathogen <named-content content-type="genus-species">Verticillium dahliae</named-content>
title_sort	transcription factor vdhapx controls iron homeostasis and is crucial for virulence in the vascular pathogen <named-content content-type="genus-species">verticillium dahliae</named-content>
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/6b6f872451774b16b28f52e8060fa788
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The Transcription Factor VdHapX Controls Iron Homeostasis and Is Crucial for Virulence in the Vascular Pathogen <named-content content-type="genus-species">Verticillium dahliae</named-content>

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