Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions

Limosilactobacillus reuteri produces reuterin via glycerol anaerobic fermentation. This compound has antimicrobial properties and is used for food preservation purposes. Filmogenic solutions constituted of polysaccharides and glycerol are also employed, however, reuterin synthesis in filmogenic solu...

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Autores principales:	F.J. Rodrigues, M.F. Cedran, J.L. Bicas, H.H. Sato
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	3-Hydroxypropionaldehyde Lactobacillus reuteri Film-forming solution In situ reuterin production Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/6b73bdefb9ac4be3badb586b1943a66e
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spelling	oai:doaj.org-article:6b73bdefb9ac4be3badb586b1943a66e2021-12-04T04:35:55ZReuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions2665-927110.1016/j.crfs.2021.11.013https://doaj.org/article/6b73bdefb9ac4be3badb586b1943a66e2021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2665927121001027https://doaj.org/toc/2665-9271Limosilactobacillus reuteri produces reuterin via glycerol anaerobic fermentation. This compound has antimicrobial properties and is used for food preservation purposes. Filmogenic solutions constituted of polysaccharides and glycerol are also employed, however, reuterin synthesis in filmogenic solutions has not yet been reported. Thus, the aim of this study was to optimize the in situ reuterin production by L. reuteri in alginate- and glycerol based-filmogenic solution, evaluating the survival of reuterin-producing bacteria during fermentation. The study consisted of a completely randomized design employing two L. reuteri strains (DSM 20016 and DSM 17938). The filmogenic solutions were obtained using sodium alginate (20 g/L) and two independent variables were studied: glycerol (0–300 mmol/L) and initial biomass of L. reuteri (≅6, 7, and 8 log CFU/mL). The samples were analyzed every 24 h for 72 h of anaerobic fermentation (37 °C). Both L. reuteri strains confirmed the potential for reuterin production and were susceptible to the metabolite produced. The highest reuterin production was achieved using L. reuteri DSM 20016. The initial microbial biomass of 8 log CFU/mL and 100 mmol/L of glycerol increased the reuterin production. However, higher conversion yields from glycerol to reuterin were obtained using 50 mmol/L of substrate.F.J. RodriguesM.F. CedranJ.L. BicasH.H. SatoElsevierarticle3-HydroxypropionaldehydeLactobacillus reuteriFilm-forming solutionIn situ reuterin productionNutrition. Foods and food supplyTX341-641Food processing and manufactureTP368-456ENCurrent Research in Food Science, Vol 4, Iss , Pp 926-931 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	3-Hydroxypropionaldehyde Lactobacillus reuteri Film-forming solution In situ reuterin production Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
spellingShingle	3-Hydroxypropionaldehyde Lactobacillus reuteri Film-forming solution In situ reuterin production Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 F.J. Rodrigues M.F. Cedran J.L. Bicas H.H. Sato Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions
description	Limosilactobacillus reuteri produces reuterin via glycerol anaerobic fermentation. This compound has antimicrobial properties and is used for food preservation purposes. Filmogenic solutions constituted of polysaccharides and glycerol are also employed, however, reuterin synthesis in filmogenic solutions has not yet been reported. Thus, the aim of this study was to optimize the in situ reuterin production by L. reuteri in alginate- and glycerol based-filmogenic solution, evaluating the survival of reuterin-producing bacteria during fermentation. The study consisted of a completely randomized design employing two L. reuteri strains (DSM 20016 and DSM 17938). The filmogenic solutions were obtained using sodium alginate (20 g/L) and two independent variables were studied: glycerol (0–300 mmol/L) and initial biomass of L. reuteri (≅6, 7, and 8 log CFU/mL). The samples were analyzed every 24 h for 72 h of anaerobic fermentation (37 °C). Both L. reuteri strains confirmed the potential for reuterin production and were susceptible to the metabolite produced. The highest reuterin production was achieved using L. reuteri DSM 20016. The initial microbial biomass of 8 log CFU/mL and 100 mmol/L of glycerol increased the reuterin production. However, higher conversion yields from glycerol to reuterin were obtained using 50 mmol/L of substrate.
format	article
author	F.J. Rodrigues M.F. Cedran J.L. Bicas H.H. Sato
author_facet	F.J. Rodrigues M.F. Cedran J.L. Bicas H.H. Sato
author_sort	F.J. Rodrigues
title	Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions
title_short	Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions
title_full	Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions
title_fullStr	Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions
title_full_unstemmed	Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions
title_sort	reuterin-producing limosilactobacillus reuteri: optimization of in situ reuterin production in alginate-based filmogenic solutions
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/6b73bdefb9ac4be3badb586b1943a66e
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Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions

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