Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease
Adherent invasive Escherichia Coli (AIEC) has been implicated in the pathogenesis of Crohn’s disease (CD) in Western populations. Whether the presence of AIEC is also seen in CD populations of different genetic susceptibility and has negative impact on host microbiota ecology and therapeutics are un...
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Taylor & Francis Group
2021
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oai:doaj.org-article:6b7c9b2d5c8b44a68d038f2a48442aa72021-11-26T11:19:48ZAssociation of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease1949-09761949-098410.1080/19490976.2021.1994833https://doaj.org/article/6b7c9b2d5c8b44a68d038f2a48442aa72021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/19490976.2021.1994833https://doaj.org/toc/1949-0976https://doaj.org/toc/1949-0984Adherent invasive Escherichia Coli (AIEC) has been implicated in the pathogenesis of Crohn’s disease (CD) in Western populations. Whether the presence of AIEC is also seen in CD populations of different genetic susceptibility and has negative impact on host microbiota ecology and therapeutics are unclear. AIEC presence was assessed in ileal tissues of 60 Hong Kong Chinese patients with CD and 56 healthy subjects. Mucosa microbiota was analyzed by 16s rRNA sequencing. Impact of AIEC on the gut microbiota was determined in a mouse model. AIEC was significantly more prevalent in ileal tissues of patients with CD than controls (30% vs 7.1%). Presence of AIEC in ileal tissues was associated with more severe mucosa microbiota dysbiosis in CD with decreased diversity and lower abundance of Firmicutes including butyrate producing Roseburia and probiotic Bacillus. A random forest model predicted the presence of AIEC with area under the curve of 0.89. AIEC exacerbated dysbiosis in dextran sodium sulfate (DSS)-induced colitis mice and led to resistance to restoration of normal gut microbiota by fecal microbiota transplantation (FMT). Proportion of donor-derived bacteria in AIEC-colonized mice was significantly lower than that in uninfected mice. AIEC was prevalent and associated with severe mucosa microbiota dysbiosis in CD in Hong Kong Chinese population. The presence of AIEC impeded restoration of normal gut microbiota. AIEC may serve as a keystone bacterium in CD and impact the efficacy of FMT.Xu ZhiluDong XiangqianYang KeliChevarin CarolineZhang JingwanLin YuZuo TaoChu Lok CheungSun YangZhang FengruiChan Francis KlSung Joseph JyYu JunBuisson AnthonyBarnich NicolasColombel Jean-FrédéricWong Sunny HeiMiao YingleiNg Siew CTaylor & Francis Grouparticleadherent-invasive escherichia coliaieccrohn’s diseaseinflammatory bowel diseasemicrobiotaDiseases of the digestive system. GastroenterologyRC799-869ENGut Microbes, Vol 13, Iss 1 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
adherent-invasive escherichia coli aiec crohn’s disease inflammatory bowel disease microbiota Diseases of the digestive system. Gastroenterology RC799-869 |
| spellingShingle |
adherent-invasive escherichia coli aiec crohn’s disease inflammatory bowel disease microbiota Diseases of the digestive system. Gastroenterology RC799-869 Xu Zhilu Dong Xiangqian Yang Keli Chevarin Caroline Zhang Jingwan Lin Yu Zuo Tao Chu Lok Cheung Sun Yang Zhang Fengrui Chan Francis Kl Sung Joseph Jy Yu Jun Buisson Anthony Barnich Nicolas Colombel Jean-Frédéric Wong Sunny Hei Miao Yinglei Ng Siew C Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
| description |
Adherent invasive Escherichia Coli (AIEC) has been implicated in the pathogenesis of Crohn’s disease (CD) in Western populations. Whether the presence of AIEC is also seen in CD populations of different genetic susceptibility and has negative impact on host microbiota ecology and therapeutics are unclear. AIEC presence was assessed in ileal tissues of 60 Hong Kong Chinese patients with CD and 56 healthy subjects. Mucosa microbiota was analyzed by 16s rRNA sequencing. Impact of AIEC on the gut microbiota was determined in a mouse model. AIEC was significantly more prevalent in ileal tissues of patients with CD than controls (30% vs 7.1%). Presence of AIEC in ileal tissues was associated with more severe mucosa microbiota dysbiosis in CD with decreased diversity and lower abundance of Firmicutes including butyrate producing Roseburia and probiotic Bacillus. A random forest model predicted the presence of AIEC with area under the curve of 0.89. AIEC exacerbated dysbiosis in dextran sodium sulfate (DSS)-induced colitis mice and led to resistance to restoration of normal gut microbiota by fecal microbiota transplantation (FMT). Proportion of donor-derived bacteria in AIEC-colonized mice was significantly lower than that in uninfected mice. AIEC was prevalent and associated with severe mucosa microbiota dysbiosis in CD in Hong Kong Chinese population. The presence of AIEC impeded restoration of normal gut microbiota. AIEC may serve as a keystone bacterium in CD and impact the efficacy of FMT. |
| format |
article |
| author |
Xu Zhilu Dong Xiangqian Yang Keli Chevarin Caroline Zhang Jingwan Lin Yu Zuo Tao Chu Lok Cheung Sun Yang Zhang Fengrui Chan Francis Kl Sung Joseph Jy Yu Jun Buisson Anthony Barnich Nicolas Colombel Jean-Frédéric Wong Sunny Hei Miao Yinglei Ng Siew C |
| author_facet |
Xu Zhilu Dong Xiangqian Yang Keli Chevarin Caroline Zhang Jingwan Lin Yu Zuo Tao Chu Lok Cheung Sun Yang Zhang Fengrui Chan Francis Kl Sung Joseph Jy Yu Jun Buisson Anthony Barnich Nicolas Colombel Jean-Frédéric Wong Sunny Hei Miao Yinglei Ng Siew C |
| author_sort |
Xu Zhilu |
| title |
Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
| title_short |
Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
| title_full |
Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
| title_fullStr |
Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
| title_full_unstemmed |
Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
| title_sort |
association of adherent-invasive escherichia coli with severe gut mucosal dysbiosis in hong kong chinese population with crohn’s disease |
| publisher |
Taylor & Francis Group |
| publishDate |
2021 |
| url |
https://doaj.org/article/6b7c9b2d5c8b44a68d038f2a48442aa7 |
| work_keys_str_mv |
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