One-Step Preparative Separation of Flavone and Isoﬂavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model

Background: Sophora japonica L. (Leguminosae), a well-known traditional medicine in Asia, is offcially listed in the Chinese and European Pharmacopoeia. Its buds and fruits have been used as medicinal agents with signifcant biological activity. Pharmacologic studies and clinical practice have demons...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhao Wang, Yang Liu, Chen Fan, Xueli Cao
Formato:	article
Lenguaje:	EN
Publicado:	Shiraz University of Medical Sciences 2021
Materias:	flavonoid glycosides sophora japonica counter-current chromatography Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/6b7fdff1b0ef41f18bd436be0b789537
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6b7fdff1b0ef41f18bd436be0b789537
record_format	dspace
spelling	oai:doaj.org-article:6b7fdff1b0ef41f18bd436be0b7895372021-11-04T07:40:11ZOne-Step Preparative Separation of Flavone and Isoﬂavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model2538-18732538-282910.30476/ijns.2021.92070.1146https://doaj.org/article/6b7fdff1b0ef41f18bd436be0b7895372021-09-01T00:00:00Zhttps://ijns.sums.ac.ir/article_47837_986a5f8e7442dbb89c2c324247ed8454.pdfhttps://doaj.org/toc/2538-1873https://doaj.org/toc/2538-2829Background: Sophora japonica L. (Leguminosae), a well-known traditional medicine in Asia, is offcially listed in the Chinese and European Pharmacopoeia. Its buds and fruits have been used as medicinal agents with signifcant biological activity. Pharmacologic studies and clinical practice have demonstrated that they have benefcial effects in the treatment of many diseases. Herein, we developed a simpler and effective separation method of ﬂavones and isoﬂavones from S. japonica fruit by one-step HSCCC based on the COSMO-RS model.Methods: HSCCC was applied for the preparative separation and purifcation of fve active compounds from S. japonica L. fruit (three ﬂavone glycosides and two isoﬂavone glycosides). Under the assistance of a COSMO-RS, one-step separation with the two-elution solvent system that was designed.Results: The frst elution was conducted with the lower phase of ethyl acetate-n-butanol-water (5:1:5, v/v), and the second elution was performed with the same one containing 4.0 mmol L-1 of NH3·H2O. From 200 mg of crude extract, 11.2 mg of kaempferol-3-O sophoroside (I), 3.2 mg of rutin (II), 3.4 mg of kaempferol-3-O-α-L-ramnopyranosyl-(1–6)-β-Dglucopyranoside (III), 11.5 mg of sophorabioside (IV), and 10.1 mg of sophoricoside (V) were obtained. Their purity values were 98.1%, 97.6%, 99.5%, 99.2%, and 98.3%, respectively.Conclusion: The fve targets of I-V can be used as reference substances for chromatographic purposes as well as for the further physiological studies. The developed method is instructive for the separation of other ﬂavonoids too.Zhao WangYang LiuChen FanXueli CaoShiraz University of Medical Sciencesarticleflavonoidglycosidessophora japonicacounter-current chromatographyNutritional diseases. Deficiency diseasesRC620-627ENInternational Journal of Nutrition Sciences, Vol 6, Iss 3, Pp 148-154 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	flavonoid glycosides sophora japonica counter-current chromatography Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	flavonoid glycosides sophora japonica counter-current chromatography Nutritional diseases. Deficiency diseases RC620-627 Zhao Wang Yang Liu Chen Fan Xueli Cao One-Step Preparative Separation of Flavone and Isoﬂavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model
description	Background: Sophora japonica L. (Leguminosae), a well-known traditional medicine in Asia, is offcially listed in the Chinese and European Pharmacopoeia. Its buds and fruits have been used as medicinal agents with signifcant biological activity. Pharmacologic studies and clinical practice have demonstrated that they have benefcial effects in the treatment of many diseases. Herein, we developed a simpler and effective separation method of ﬂavones and isoﬂavones from S. japonica fruit by one-step HSCCC based on the COSMO-RS model.Methods: HSCCC was applied for the preparative separation and purifcation of fve active compounds from S. japonica L. fruit (three ﬂavone glycosides and two isoﬂavone glycosides). Under the assistance of a COSMO-RS, one-step separation with the two-elution solvent system that was designed.Results: The frst elution was conducted with the lower phase of ethyl acetate-n-butanol-water (5:1:5, v/v), and the second elution was performed with the same one containing 4.0 mmol L-1 of NH3·H2O. From 200 mg of crude extract, 11.2 mg of kaempferol-3-O sophoroside (I), 3.2 mg of rutin (II), 3.4 mg of kaempferol-3-O-α-L-ramnopyranosyl-(1–6)-β-Dglucopyranoside (III), 11.5 mg of sophorabioside (IV), and 10.1 mg of sophoricoside (V) were obtained. Their purity values were 98.1%, 97.6%, 99.5%, 99.2%, and 98.3%, respectively.Conclusion: The fve targets of I-V can be used as reference substances for chromatographic purposes as well as for the further physiological studies. The developed method is instructive for the separation of other ﬂavonoids too.
format	article
author	Zhao Wang Yang Liu Chen Fan Xueli Cao
author_facet	Zhao Wang Yang Liu Chen Fan Xueli Cao
author_sort	Zhao Wang
title	One-Step Preparative Separation of Flavone and Isoﬂavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model
title_short	One-Step Preparative Separation of Flavone and Isoﬂavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model
title_full	One-Step Preparative Separation of Flavone and Isoﬂavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model
title_fullStr	One-Step Preparative Separation of Flavone and Isoﬂavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model
title_full_unstemmed	One-Step Preparative Separation of Flavone and Isoﬂavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model
title_sort	one-step preparative separation of flavone and isoﬂavone glycosides from sophora japonica fruit by high-speed counter-current chromatography based on cosmo-rs model
publisher	Shiraz University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/6b7fdff1b0ef41f18bd436be0b789537
work_keys_str_mv	AT zhaowang onesteppreparativeseparationofflavoneandisoflavoneglycosidesfromsophorajaponicafruitbyhighspeedcountercurrentchromatographybasedoncosmorsmodel AT yangliu onesteppreparativeseparationofflavoneandisoflavoneglycosidesfromsophorajaponicafruitbyhighspeedcountercurrentchromatographybasedoncosmorsmodel AT chenfan onesteppreparativeseparationofflavoneandisoflavoneglycosidesfromsophorajaponicafruitbyhighspeedcountercurrentchromatographybasedoncosmorsmodel AT xuelicao onesteppreparativeseparationofflavoneandisoflavoneglycosidesfromsophorajaponicafruitbyhighspeedcountercurrentchromatographybasedoncosmorsmodel
_version_	1718445026129739776

One-Step Preparative Separation of Flavone and Isoﬂavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model

Ejemplares similares