Full-Length Transcriptomics Reveal the Gene Expression Profiles of Reef-Building Coral <i>Pocillopora damicornis</i> and Symbiont Zooxanthellae

Since the last century, episodes of coral reef bleaching and mortality have occurred almost annually in tropical or subtropical seas. When the temperature exceeds the tolerant limit of a coral–zooxanthellae holobiont, it induces physiological stress and disrupts the vulnerable fine-tuned balance bet...

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Autores principales:	Zhuojun Guo, Xin Liao, Tingyu Han, Junyuan Chen, Chunpeng He, Zuhong Lu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	full-length transcriptome gene expression profile reef-building coral zooxanthellae holobiont <i>Pocillopora damicornis</i> Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6b8de2018b554673b5da3aa88bb3a8cd
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spelling	oai:doaj.org-article:6b8de2018b554673b5da3aa88bb3a8cd2021-11-25T17:22:31ZFull-Length Transcriptomics Reveal the Gene Expression Profiles of Reef-Building Coral <i>Pocillopora damicornis</i> and Symbiont Zooxanthellae10.3390/d131105431424-2818https://doaj.org/article/6b8de2018b554673b5da3aa88bb3a8cd2021-10-01T00:00:00Zhttps://www.mdpi.com/1424-2818/13/11/543https://doaj.org/toc/1424-2818Since the last century, episodes of coral reef bleaching and mortality have occurred almost annually in tropical or subtropical seas. When the temperature exceeds the tolerant limit of a coral–zooxanthellae holobiont, it induces physiological stress and disrupts the vulnerable fine-tuned balance between the two partners, leading to bleaching. The gene expression profiles of a scleractinian coral and its symbiotic zooxanthellae can offer important information with which to decipher this balanced relationship at the functional level of genes. Here, we sequence a full-length transcriptome of a well-known, common and frequently dominant reef-building coral, <i>Pocillopora damicornis</i>, to acquire gene expression information for the coral–zooxanthellae holobiont. To this end, we identify 21,926 and 465 unique genes in the coral and algal symbiont, respectively, and examine the functional enrichment among these genes based on GO (gene ontology) terms and KEGG (the Kyoto Encyclopedia of Genes and Genomes) pathways. The results show that the zooxanthellae provide for their coral host through energy and nutrition metabolism by photosynthesis, and that both the coral host and zooxanthellae have an anti-stress molecular mechanism, though the two parties have independent abilities to survive in the short term. This work sheds light on the valuable gene expression profile of a coral–zooxanthellae holobiont and provides grounds for further molecular biological research to support ecological protection work.Zhuojun GuoXin LiaoTingyu HanJunyuan ChenChunpeng HeZuhong LuMDPI AGarticlefull-length transcriptomegene expression profilereef-building coralzooxanthellaeholobiont<i>Pocillopora damicornis</i>Biology (General)QH301-705.5ENDiversity, Vol 13, Iss 543, p 543 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	full-length transcriptome gene expression profile reef-building coral zooxanthellae holobiont <i>Pocillopora damicornis</i> Biology (General) QH301-705.5
spellingShingle	full-length transcriptome gene expression profile reef-building coral zooxanthellae holobiont <i>Pocillopora damicornis</i> Biology (General) QH301-705.5 Zhuojun Guo Xin Liao Tingyu Han Junyuan Chen Chunpeng He Zuhong Lu Full-Length Transcriptomics Reveal the Gene Expression Profiles of Reef-Building Coral <i>Pocillopora damicornis</i> and Symbiont Zooxanthellae
description	Since the last century, episodes of coral reef bleaching and mortality have occurred almost annually in tropical or subtropical seas. When the temperature exceeds the tolerant limit of a coral–zooxanthellae holobiont, it induces physiological stress and disrupts the vulnerable fine-tuned balance between the two partners, leading to bleaching. The gene expression profiles of a scleractinian coral and its symbiotic zooxanthellae can offer important information with which to decipher this balanced relationship at the functional level of genes. Here, we sequence a full-length transcriptome of a well-known, common and frequently dominant reef-building coral, <i>Pocillopora damicornis</i>, to acquire gene expression information for the coral–zooxanthellae holobiont. To this end, we identify 21,926 and 465 unique genes in the coral and algal symbiont, respectively, and examine the functional enrichment among these genes based on GO (gene ontology) terms and KEGG (the Kyoto Encyclopedia of Genes and Genomes) pathways. The results show that the zooxanthellae provide for their coral host through energy and nutrition metabolism by photosynthesis, and that both the coral host and zooxanthellae have an anti-stress molecular mechanism, though the two parties have independent abilities to survive in the short term. This work sheds light on the valuable gene expression profile of a coral–zooxanthellae holobiont and provides grounds for further molecular biological research to support ecological protection work.
format	article
author	Zhuojun Guo Xin Liao Tingyu Han Junyuan Chen Chunpeng He Zuhong Lu
author_facet	Zhuojun Guo Xin Liao Tingyu Han Junyuan Chen Chunpeng He Zuhong Lu
author_sort	Zhuojun Guo
title	Full-Length Transcriptomics Reveal the Gene Expression Profiles of Reef-Building Coral <i>Pocillopora damicornis</i> and Symbiont Zooxanthellae
title_short	Full-Length Transcriptomics Reveal the Gene Expression Profiles of Reef-Building Coral <i>Pocillopora damicornis</i> and Symbiont Zooxanthellae
title_full	Full-Length Transcriptomics Reveal the Gene Expression Profiles of Reef-Building Coral <i>Pocillopora damicornis</i> and Symbiont Zooxanthellae
title_fullStr	Full-Length Transcriptomics Reveal the Gene Expression Profiles of Reef-Building Coral <i>Pocillopora damicornis</i> and Symbiont Zooxanthellae
title_full_unstemmed	Full-Length Transcriptomics Reveal the Gene Expression Profiles of Reef-Building Coral <i>Pocillopora damicornis</i> and Symbiont Zooxanthellae
title_sort	full-length transcriptomics reveal the gene expression profiles of reef-building coral <i>pocillopora damicornis</i> and symbiont zooxanthellae
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6b8de2018b554673b5da3aa88bb3a8cd
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Full-Length Transcriptomics Reveal the Gene Expression Profiles of Reef-Building Coral <i>Pocillopora damicornis</i> and Symbiont Zooxanthellae

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