Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan
The acquisition of drug resistance and virulence by staphylococcal species colonizing humans is a growing public health concern. The present study was conducted to investigate the prevalence, antimicrobial resistance and genetic characteristics of <i>Staphylococcus</i> isolates from the...
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| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/6b9c3b6afddd48fc80677626d16f13a7 |
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| Sumario: | The acquisition of drug resistance and virulence by staphylococcal species colonizing humans is a growing public health concern. The present study was conducted to investigate the prevalence, antimicrobial resistance and genetic characteristics of <i>Staphylococcus</i> isolates from the oral cavity and skin (hand) of systemically healthy subjects with dental disease and dental staff in northern Japan. Among a total of 133 subjects (91 patients and 42 staff), 87 coagulase-positive <i>Staphylococcus</i> (83 <i>S. aureus</i>/4 <i>S. argenteus</i>) and 162 coagulase-negative <i>Staphylococcus</i> (CoNS) isolates were recovered from 59 (44.4%) and 95 (71.4%) subjects, respectively. Three oral isolates were methicillin-resistant <i>S. aureus</i> (MRSA) (3.6%, 3/83) that were genotyped as ST8-SCC<i>mec</i>-IVl, ST4775(CC1)-SCC<i>mec</i>-IVa and ST6562(CC8)-SCC<i>mec</i>-IVa. Remarkably, the ST6562 isolate harbored PVL genes on ΦSa2usa and type I ACME (arginine catabolic mobile element). Four methicillin-susceptible isolates were identified as <i>S. argenteus</i> belonging to ST1223 and ST2250, which harbored enterotoxin genes <i>egc-2</i> and <i>sey</i>, respectively. Among the fourteen CoNS species identified, methicillin-resistant (MR) isolates were detected in five species (11 isolates, 13.3% of CoNS), with <i>S. saprophyticus</i> and <i>S. haemolyticus</i> being the most common. ACME was prevalent in only <i>S. epidermidis</i> and <i>S. capitis</i>. These findings indicated the potential distribution of USA300 clone-like MRSA, toxigenic <i>S. argenteus</i> and MR-CoNS in the oral cavity of dental patients. |
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