Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan

The acquisition of drug resistance and virulence by staphylococcal species colonizing humans is a growing public health concern. The present study was conducted to investigate the prevalence, antimicrobial resistance and genetic characteristics of <i>Staphylococcus</i> isolates from the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mina Hirose, Meiji Soe Aung, Atsushi Fukuda, Shoko Yahata, Yusuke Fujita, Masato Saitoh, Yukito Hirose, Noriko Urushibara, Nobumichi Kobayashi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	MRSA <i>Staphylococcus argenteus</i> coagulase-negative <i>Staphylococcus</i> oral cavity hand SCC<i>mec</i> Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/6b9c3b6afddd48fc80677626d16f13a7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6b9c3b6afddd48fc80677626d16f13a7
record_format	dspace
spelling	oai:doaj.org-article:6b9c3b6afddd48fc80677626d16f13a72021-11-25T16:22:40ZAntimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan10.3390/antibiotics101113162079-6382https://doaj.org/article/6b9c3b6afddd48fc80677626d16f13a72021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1316https://doaj.org/toc/2079-6382The acquisition of drug resistance and virulence by staphylococcal species colonizing humans is a growing public health concern. The present study was conducted to investigate the prevalence, antimicrobial resistance and genetic characteristics of <i>Staphylococcus</i> isolates from the oral cavity and skin (hand) of systemically healthy subjects with dental disease and dental staff in northern Japan. Among a total of 133 subjects (91 patients and 42 staff), 87 coagulase-positive <i>Staphylococcus</i> (83 <i>S. aureus</i>/4 <i>S. argenteus</i>) and 162 coagulase-negative <i>Staphylococcus</i> (CoNS) isolates were recovered from 59 (44.4%) and 95 (71.4%) subjects, respectively. Three oral isolates were methicillin-resistant <i>S. aureus</i> (MRSA) (3.6%, 3/83) that were genotyped as ST8-SCC<i>mec</i>-IVl, ST4775(CC1)-SCC<i>mec</i>-IVa and ST6562(CC8)-SCC<i>mec</i>-IVa. Remarkably, the ST6562 isolate harbored PVL genes on ΦSa2usa and type I ACME (arginine catabolic mobile element). Four methicillin-susceptible isolates were identified as <i>S. argenteus</i> belonging to ST1223 and ST2250, which harbored enterotoxin genes <i>egc-2</i> and <i>sey</i>, respectively. Among the fourteen CoNS species identified, methicillin-resistant (MR) isolates were detected in five species (11 isolates, 13.3% of CoNS), with <i>S. saprophyticus</i> and <i>S. haemolyticus</i> being the most common. ACME was prevalent in only <i>S. epidermidis</i> and <i>S. capitis</i>. These findings indicated the potential distribution of USA300 clone-like MRSA, toxigenic <i>S. argenteus</i> and MR-CoNS in the oral cavity of dental patients.Mina HiroseMeiji Soe AungAtsushi FukudaShoko YahataYusuke FujitaMasato SaitohYukito HiroseNoriko UrushibaraNobumichi KobayashiMDPI AGarticleMRSA<i>Staphylococcus argenteus</i>coagulase-negative <i>Staphylococcus</i>oral cavityhandSCC<i>mec</i>Therapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1316, p 1316 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MRSA <i>Staphylococcus argenteus</i> coagulase-negative <i>Staphylococcus</i> oral cavity hand SCC<i>mec</i> Therapeutics. Pharmacology RM1-950
spellingShingle	MRSA <i>Staphylococcus argenteus</i> coagulase-negative <i>Staphylococcus</i> oral cavity hand SCC<i>mec</i> Therapeutics. Pharmacology RM1-950 Mina Hirose Meiji Soe Aung Atsushi Fukuda Shoko Yahata Yusuke Fujita Masato Saitoh Yukito Hirose Noriko Urushibara Nobumichi Kobayashi Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan
description	The acquisition of drug resistance and virulence by staphylococcal species colonizing humans is a growing public health concern. The present study was conducted to investigate the prevalence, antimicrobial resistance and genetic characteristics of <i>Staphylococcus</i> isolates from the oral cavity and skin (hand) of systemically healthy subjects with dental disease and dental staff in northern Japan. Among a total of 133 subjects (91 patients and 42 staff), 87 coagulase-positive <i>Staphylococcus</i> (83 <i>S. aureus</i>/4 <i>S. argenteus</i>) and 162 coagulase-negative <i>Staphylococcus</i> (CoNS) isolates were recovered from 59 (44.4%) and 95 (71.4%) subjects, respectively. Three oral isolates were methicillin-resistant <i>S. aureus</i> (MRSA) (3.6%, 3/83) that were genotyped as ST8-SCC<i>mec</i>-IVl, ST4775(CC1)-SCC<i>mec</i>-IVa and ST6562(CC8)-SCC<i>mec</i>-IVa. Remarkably, the ST6562 isolate harbored PVL genes on ΦSa2usa and type I ACME (arginine catabolic mobile element). Four methicillin-susceptible isolates were identified as <i>S. argenteus</i> belonging to ST1223 and ST2250, which harbored enterotoxin genes <i>egc-2</i> and <i>sey</i>, respectively. Among the fourteen CoNS species identified, methicillin-resistant (MR) isolates were detected in five species (11 isolates, 13.3% of CoNS), with <i>S. saprophyticus</i> and <i>S. haemolyticus</i> being the most common. ACME was prevalent in only <i>S. epidermidis</i> and <i>S. capitis</i>. These findings indicated the potential distribution of USA300 clone-like MRSA, toxigenic <i>S. argenteus</i> and MR-CoNS in the oral cavity of dental patients.
format	article
author	Mina Hirose Meiji Soe Aung Atsushi Fukuda Shoko Yahata Yusuke Fujita Masato Saitoh Yukito Hirose Noriko Urushibara Nobumichi Kobayashi
author_facet	Mina Hirose Meiji Soe Aung Atsushi Fukuda Shoko Yahata Yusuke Fujita Masato Saitoh Yukito Hirose Noriko Urushibara Nobumichi Kobayashi
author_sort	Mina Hirose
title	Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan
title_short	Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan
title_full	Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan
title_fullStr	Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan
title_full_unstemmed	Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan
title_sort	antimicrobial resistance and molecular epidemiological characteristics of methicillin-resistant and susceptible staphylococcal isolates from oral cavity of dental patients and staff in northern japan
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6b9c3b6afddd48fc80677626d16f13a7
work_keys_str_mv	AT minahirose antimicrobialresistanceandmolecularepidemiologicalcharacteristicsofmethicillinresistantandsusceptiblestaphylococcalisolatesfromoralcavityofdentalpatientsandstaffinnorthernjapan AT meijisoeaung antimicrobialresistanceandmolecularepidemiologicalcharacteristicsofmethicillinresistantandsusceptiblestaphylococcalisolatesfromoralcavityofdentalpatientsandstaffinnorthernjapan AT atsushifukuda antimicrobialresistanceandmolecularepidemiologicalcharacteristicsofmethicillinresistantandsusceptiblestaphylococcalisolatesfromoralcavityofdentalpatientsandstaffinnorthernjapan AT shokoyahata antimicrobialresistanceandmolecularepidemiologicalcharacteristicsofmethicillinresistantandsusceptiblestaphylococcalisolatesfromoralcavityofdentalpatientsandstaffinnorthernjapan AT yusukefujita antimicrobialresistanceandmolecularepidemiologicalcharacteristicsofmethicillinresistantandsusceptiblestaphylococcalisolatesfromoralcavityofdentalpatientsandstaffinnorthernjapan AT masatosaitoh antimicrobialresistanceandmolecularepidemiologicalcharacteristicsofmethicillinresistantandsusceptiblestaphylococcalisolatesfromoralcavityofdentalpatientsandstaffinnorthernjapan AT yukitohirose antimicrobialresistanceandmolecularepidemiologicalcharacteristicsofmethicillinresistantandsusceptiblestaphylococcalisolatesfromoralcavityofdentalpatientsandstaffinnorthernjapan AT norikourushibara antimicrobialresistanceandmolecularepidemiologicalcharacteristicsofmethicillinresistantandsusceptiblestaphylococcalisolatesfromoralcavityofdentalpatientsandstaffinnorthernjapan AT nobumichikobayashi antimicrobialresistanceandmolecularepidemiologicalcharacteristicsofmethicillinresistantandsusceptiblestaphylococcalisolatesfromoralcavityofdentalpatientsandstaffinnorthernjapan
_version_	1718413204943536128

Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan

Ejemplares similares