Comparative genome analysis of Megasphaera sp. reveals niche specialization and its potential role in the human gut.

With increasing number of novel bacteria being isolated from the human gut ecosystem, there is a greater need to study their role in the gut ecosystem and their effect on the host health. In the present study, we carried out in silico genome-wide analysis of two novel Megasphaera sp. isolates NM10 (...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sudarshan Anand Shetty, Nachiket Prakash Marathe, Vikram Lanjekar, Dilip Ranade, Yogesh S Shouche
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ba57a8c98fb418889a1629147778efa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6ba57a8c98fb418889a1629147778efa
record_format	dspace
spelling	oai:doaj.org-article:6ba57a8c98fb418889a1629147778efa2021-11-18T08:45:53ZComparative genome analysis of Megasphaera sp. reveals niche specialization and its potential role in the human gut.1932-620310.1371/journal.pone.0079353https://doaj.org/article/6ba57a8c98fb418889a1629147778efa2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24260205/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203With increasing number of novel bacteria being isolated from the human gut ecosystem, there is a greater need to study their role in the gut ecosystem and their effect on the host health. In the present study, we carried out in silico genome-wide analysis of two novel Megasphaera sp. isolates NM10 (DSM25563) and BL7 (DSM25562), isolated from feces of two healthy individuals and validated the key features by in vitro studies. The analysis revealed the general metabolic potential, adaptive features and the potential effects of these isolates on the host. The comparative genome analysis of the two human gut isolates NM10 and BL7 with ruminal isolate Megasphaera elsdenii (DSM20460) highlighted the differential adaptive features for their survival in human gut. The key findings include features like bile resistance, presence of various sensory and regulatory systems, stress response systems, membrane transporters and resistance to antibiotics. Comparison of the "glycobiome" based on the genomes of the ruminal isolate with the human gut isolates NM10 and BL revealed the presence of diverse and unique sets of Carbohydrate-Active enzymes (CAZymes) amongst these isolates, with a higher collection of CAZymes in the human gut isolates. This could be attributed to the difference in host diet and thereby the environment, consequently suggesting host specific adaptation in these isolates. In silico analysis of metabolic potential predicted the ability of these isolates to produce important metabolites like short chain fatty acids (butyrate, acetate, formate, and caproate), vitamins and essential amino acids, which was further validated by in vitro experiments. The ability of these isolates to produce important metabolites advocates for a potential healthy influence on the host. Further in vivo studies including transcriptomic and proteomic analysis will be required for better understanding the role and impact of these Megasphaera sp. isolates NM10 and BL7 on the human host.Sudarshan Anand ShettyNachiket Prakash MaratheVikram LanjekarDilip RanadeYogesh S ShouchePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79353 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sudarshan Anand Shetty Nachiket Prakash Marathe Vikram Lanjekar Dilip Ranade Yogesh S Shouche Comparative genome analysis of Megasphaera sp. reveals niche specialization and its potential role in the human gut.
description	With increasing number of novel bacteria being isolated from the human gut ecosystem, there is a greater need to study their role in the gut ecosystem and their effect on the host health. In the present study, we carried out in silico genome-wide analysis of two novel Megasphaera sp. isolates NM10 (DSM25563) and BL7 (DSM25562), isolated from feces of two healthy individuals and validated the key features by in vitro studies. The analysis revealed the general metabolic potential, adaptive features and the potential effects of these isolates on the host. The comparative genome analysis of the two human gut isolates NM10 and BL7 with ruminal isolate Megasphaera elsdenii (DSM20460) highlighted the differential adaptive features for their survival in human gut. The key findings include features like bile resistance, presence of various sensory and regulatory systems, stress response systems, membrane transporters and resistance to antibiotics. Comparison of the "glycobiome" based on the genomes of the ruminal isolate with the human gut isolates NM10 and BL revealed the presence of diverse and unique sets of Carbohydrate-Active enzymes (CAZymes) amongst these isolates, with a higher collection of CAZymes in the human gut isolates. This could be attributed to the difference in host diet and thereby the environment, consequently suggesting host specific adaptation in these isolates. In silico analysis of metabolic potential predicted the ability of these isolates to produce important metabolites like short chain fatty acids (butyrate, acetate, formate, and caproate), vitamins and essential amino acids, which was further validated by in vitro experiments. The ability of these isolates to produce important metabolites advocates for a potential healthy influence on the host. Further in vivo studies including transcriptomic and proteomic analysis will be required for better understanding the role and impact of these Megasphaera sp. isolates NM10 and BL7 on the human host.
format	article
author	Sudarshan Anand Shetty Nachiket Prakash Marathe Vikram Lanjekar Dilip Ranade Yogesh S Shouche
author_facet	Sudarshan Anand Shetty Nachiket Prakash Marathe Vikram Lanjekar Dilip Ranade Yogesh S Shouche
author_sort	Sudarshan Anand Shetty
title	Comparative genome analysis of Megasphaera sp. reveals niche specialization and its potential role in the human gut.
title_short	Comparative genome analysis of Megasphaera sp. reveals niche specialization and its potential role in the human gut.
title_full	Comparative genome analysis of Megasphaera sp. reveals niche specialization and its potential role in the human gut.
title_fullStr	Comparative genome analysis of Megasphaera sp. reveals niche specialization and its potential role in the human gut.
title_full_unstemmed	Comparative genome analysis of Megasphaera sp. reveals niche specialization and its potential role in the human gut.
title_sort	comparative genome analysis of megasphaera sp. reveals niche specialization and its potential role in the human gut.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6ba57a8c98fb418889a1629147778efa
work_keys_str_mv	AT sudarshananandshetty comparativegenomeanalysisofmegasphaerasprevealsnichespecializationanditspotentialroleinthehumangut AT nachiketprakashmarathe comparativegenomeanalysisofmegasphaerasprevealsnichespecializationanditspotentialroleinthehumangut AT vikramlanjekar comparativegenomeanalysisofmegasphaerasprevealsnichespecializationanditspotentialroleinthehumangut AT dilipranade comparativegenomeanalysisofmegasphaerasprevealsnichespecializationanditspotentialroleinthehumangut AT yogeshsshouche comparativegenomeanalysisofmegasphaerasprevealsnichespecializationanditspotentialroleinthehumangut
_version_	1718421326494957568

Comparative genome analysis of Megasphaera sp. reveals niche specialization and its potential role in the human gut.

Ejemplares similares