Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material

Abstract The first polyhydroxyalkanoate (PHA) block copolymer poly(2-hydroxybutyrate-b-3-hydroxybutyrate) [P(2HB-b-3HB)] was previously synthesized using engineered Escherichia coli expressing a chimeric PHA synthase PhaCAR with monomer sequence-regulating capacity. In the present study, the physica...

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Autores principales:	Yuki Kageyama, Hiroya Tomita, Takuya Isono, Toshifumi Satoh, Ken’ichiro Matsumoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ba9606d1b4642c8824c9b9896d10447
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spelling	oai:doaj.org-article:6ba9606d1b4642c8824c9b9896d104472021-11-21T12:23:07ZArtificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material10.1038/s41598-021-01828-92045-2322https://doaj.org/article/6ba9606d1b4642c8824c9b9896d104472021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01828-9https://doaj.org/toc/2045-2322Abstract The first polyhydroxyalkanoate (PHA) block copolymer poly(2-hydroxybutyrate-b-3-hydroxybutyrate) [P(2HB-b-3HB)] was previously synthesized using engineered Escherichia coli expressing a chimeric PHA synthase PhaCAR with monomer sequence-regulating capacity. In the present study, the physical properties of the block copolymer and its relevant random copolymer P(2HB-ran-3HB) were evaluated. Stress–strain tests on the P(88 mol% 2HB-b-3HB) film showed an increasing stress value during elongation up to 393%. In addition, the block copolymer film exhibited slow contraction behavior after elongation, indicating that P(2HB-b-3HB) is an elastomer-like material. In contrast, the P(92 mol% 2HB-ran-3HB) film, which was stretched up to 692% with nearly constant stress, was stretchable but not elastic. The differential scanning calorimetry and wide-angle X-ray diffraction analyses indicated that the P(2HB-b-3HB) contained the amorphous P(2HB) phase and the crystalline P(3HB) phase, whereas P(2HB-ran-3HB) was wholly amorphous. Therefore, the elasticity of P(2HB-b-3HB) can be attributed to the presence of the crystalline P(3HB) phase and a noncovalent crosslinked structure by the crystals. These results show the potential of block PHAs as elastic materials.Yuki KageyamaHiroya TomitaTakuya IsonoToshifumi SatohKen’ichiro MatsumotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuki Kageyama Hiroya Tomita Takuya Isono Toshifumi Satoh Ken’ichiro Matsumoto Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material
description	Abstract The first polyhydroxyalkanoate (PHA) block copolymer poly(2-hydroxybutyrate-b-3-hydroxybutyrate) [P(2HB-b-3HB)] was previously synthesized using engineered Escherichia coli expressing a chimeric PHA synthase PhaCAR with monomer sequence-regulating capacity. In the present study, the physical properties of the block copolymer and its relevant random copolymer P(2HB-ran-3HB) were evaluated. Stress–strain tests on the P(88 mol% 2HB-b-3HB) film showed an increasing stress value during elongation up to 393%. In addition, the block copolymer film exhibited slow contraction behavior after elongation, indicating that P(2HB-b-3HB) is an elastomer-like material. In contrast, the P(92 mol% 2HB-ran-3HB) film, which was stretched up to 692% with nearly constant stress, was stretchable but not elastic. The differential scanning calorimetry and wide-angle X-ray diffraction analyses indicated that the P(2HB-b-3HB) contained the amorphous P(2HB) phase and the crystalline P(3HB) phase, whereas P(2HB-ran-3HB) was wholly amorphous. Therefore, the elasticity of P(2HB-b-3HB) can be attributed to the presence of the crystalline P(3HB) phase and a noncovalent crosslinked structure by the crystals. These results show the potential of block PHAs as elastic materials.
format	article
author	Yuki Kageyama Hiroya Tomita Takuya Isono Toshifumi Satoh Ken’ichiro Matsumoto
author_facet	Yuki Kageyama Hiroya Tomita Takuya Isono Toshifumi Satoh Ken’ichiro Matsumoto
author_sort	Yuki Kageyama
title	Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material
title_short	Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material
title_full	Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material
title_fullStr	Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material
title_full_unstemmed	Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material
title_sort	artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6ba9606d1b4642c8824c9b9896d10447
work_keys_str_mv	AT yukikageyama artificialpolyhydroxyalkanoatepoly2hydroxybutyrateblock3hydroxybutyrateelastomerlikematerial AT hiroyatomita artificialpolyhydroxyalkanoatepoly2hydroxybutyrateblock3hydroxybutyrateelastomerlikematerial AT takuyaisono artificialpolyhydroxyalkanoatepoly2hydroxybutyrateblock3hydroxybutyrateelastomerlikematerial AT toshifumisatoh artificialpolyhydroxyalkanoatepoly2hydroxybutyrateblock3hydroxybutyrateelastomerlikematerial AT kenichiromatsumoto artificialpolyhydroxyalkanoatepoly2hydroxybutyrateblock3hydroxybutyrateelastomerlikematerial
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Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material

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