Identification of Polymorphisms of the <i>CSN2</i> Gene Encoding β-Casein in Greek Local Breeds of Cattle

This e research focused on the detection and identification of genetic polymorphisms in exon 7 of the β-casein <i>CSN2</i> gene in blood samples from Greek Holstein cows and from local breeds of cattle, such as Vrachykeratiki, Katerinis, and Sykias. For this purpose, DNA was isolated fro...

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Autores principales:	Dionysios Antonopoulos, Despina Vougiouklaki, George P. Laliotis, Theofania Tsironi, Irene Valasi, Archodoula Chatzilazarou, Panagiotis Halvatsiotis, Dimitra Houhoula
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	β-casein b-casomorphin-7 polymorphism PCR-RFLP Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/6bbaee0d7e214b3a8019921bd7093bfd
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spelling	oai:doaj.org-article:6bbaee0d7e214b3a8019921bd7093bfd2021-11-25T19:11:55ZIdentification of Polymorphisms of the <i>CSN2</i> Gene Encoding β-Casein in Greek Local Breeds of Cattle10.3390/vetsci81102572306-7381https://doaj.org/article/6bbaee0d7e214b3a8019921bd7093bfd2021-10-01T00:00:00Zhttps://www.mdpi.com/2306-7381/8/11/257https://doaj.org/toc/2306-7381This e research focused on the detection and identification of genetic polymorphisms in exon 7 of the β-casein <i>CSN2</i> gene in blood samples from Greek Holstein cows and from local breeds of cattle, such as Vrachykeratiki, Katerinis, and Sykias. For this purpose, DNA was isolated from 780 blood samples obtained from Greek Holstein cows, 86 from three local breeds of cattle, namely Brachyceros, Katerinis, and Sykias, and 14 from Greek buffalo. The desired region of exon 7 was amplified by PCR, resulting in 121 and 251 bp products in bovine and buffalo samples. The PCR product was digested with restriction fragment length polymorphism (RFLP) on agarose gels. The restriction enzymes <i>DdeI and TaqI</i> were used. All of the blood samples had the amplified size. The results showed that 74.4% of the Greek Holstein cows had the A2A2 β-casein genotype, the three native breads Vrachykeratiki had 57.7%, and the other two had 100% of the A2A2 β-casein. From the 14 Greek buffalo, 100% had the A2A2 β-casein.Dionysios AntonopoulosDespina VougiouklakiGeorge P. LaliotisTheofania TsironiIrene ValasiArchodoula ChatzilazarouPanagiotis HalvatsiotisDimitra HouhoulaMDPI AGarticleβ-caseinb-casomorphin-7polymorphismPCR-RFLPVeterinary medicineSF600-1100ENVeterinary Sciences, Vol 8, Iss 257, p 257 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	β-casein b-casomorphin-7 polymorphism PCR-RFLP Veterinary medicine SF600-1100
spellingShingle	β-casein b-casomorphin-7 polymorphism PCR-RFLP Veterinary medicine SF600-1100 Dionysios Antonopoulos Despina Vougiouklaki George P. Laliotis Theofania Tsironi Irene Valasi Archodoula Chatzilazarou Panagiotis Halvatsiotis Dimitra Houhoula Identification of Polymorphisms of the <i>CSN2</i> Gene Encoding β-Casein in Greek Local Breeds of Cattle
description	This e research focused on the detection and identification of genetic polymorphisms in exon 7 of the β-casein <i>CSN2</i> gene in blood samples from Greek Holstein cows and from local breeds of cattle, such as Vrachykeratiki, Katerinis, and Sykias. For this purpose, DNA was isolated from 780 blood samples obtained from Greek Holstein cows, 86 from three local breeds of cattle, namely Brachyceros, Katerinis, and Sykias, and 14 from Greek buffalo. The desired region of exon 7 was amplified by PCR, resulting in 121 and 251 bp products in bovine and buffalo samples. The PCR product was digested with restriction fragment length polymorphism (RFLP) on agarose gels. The restriction enzymes <i>DdeI and TaqI</i> were used. All of the blood samples had the amplified size. The results showed that 74.4% of the Greek Holstein cows had the A2A2 β-casein genotype, the three native breads Vrachykeratiki had 57.7%, and the other two had 100% of the A2A2 β-casein. From the 14 Greek buffalo, 100% had the A2A2 β-casein.
format	article
author	Dionysios Antonopoulos Despina Vougiouklaki George P. Laliotis Theofania Tsironi Irene Valasi Archodoula Chatzilazarou Panagiotis Halvatsiotis Dimitra Houhoula
author_facet	Dionysios Antonopoulos Despina Vougiouklaki George P. Laliotis Theofania Tsironi Irene Valasi Archodoula Chatzilazarou Panagiotis Halvatsiotis Dimitra Houhoula
author_sort	Dionysios Antonopoulos
title	Identification of Polymorphisms of the <i>CSN2</i> Gene Encoding β-Casein in Greek Local Breeds of Cattle
title_short	Identification of Polymorphisms of the <i>CSN2</i> Gene Encoding β-Casein in Greek Local Breeds of Cattle
title_full	Identification of Polymorphisms of the <i>CSN2</i> Gene Encoding β-Casein in Greek Local Breeds of Cattle
title_fullStr	Identification of Polymorphisms of the <i>CSN2</i> Gene Encoding β-Casein in Greek Local Breeds of Cattle
title_full_unstemmed	Identification of Polymorphisms of the <i>CSN2</i> Gene Encoding β-Casein in Greek Local Breeds of Cattle
title_sort	identification of polymorphisms of the <i>csn2</i> gene encoding β-casein in greek local breeds of cattle
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6bbaee0d7e214b3a8019921bd7093bfd
work_keys_str_mv	AT dionysiosantonopoulos identificationofpolymorphismsoftheicsn2igeneencodingbcaseiningreeklocalbreedsofcattle AT despinavougiouklaki identificationofpolymorphismsoftheicsn2igeneencodingbcaseiningreeklocalbreedsofcattle AT georgeplaliotis identificationofpolymorphismsoftheicsn2igeneencodingbcaseiningreeklocalbreedsofcattle AT theofaniatsironi identificationofpolymorphismsoftheicsn2igeneencodingbcaseiningreeklocalbreedsofcattle AT irenevalasi identificationofpolymorphismsoftheicsn2igeneencodingbcaseiningreeklocalbreedsofcattle AT archodoulachatzilazarou identificationofpolymorphismsoftheicsn2igeneencodingbcaseiningreeklocalbreedsofcattle AT panagiotishalvatsiotis identificationofpolymorphismsoftheicsn2igeneencodingbcaseiningreeklocalbreedsofcattle AT dimitrahouhoula identificationofpolymorphismsoftheicsn2igeneencodingbcaseiningreeklocalbreedsofcattle
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Identification of Polymorphisms of the <i>CSN2</i> Gene Encoding β-Casein in Greek Local Breeds of Cattle

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