Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine

Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine

Tania Patiño, Carme Nogués, Elena Ibáñez, Leonardo BarriosUnitat de Biologia Cel lular, Departament de Biologia Cel lular, Fisiologia i Immunologia, Facultat de Biociències, Universitat Autònoma de Barcelona, B...

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Autores principales: Patiño T, Nogués C, Ibáñez E, Barrios L
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2012
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/6bbc25b45bbc4a7ba7a6bd36aca23f66
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spelling oai:doaj.org-article:6bbc25b45bbc4a7ba7a6bd36aca23f662021-12-02T07:14:15ZEnhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine1176-91141178-2013https://doaj.org/article/6bbc25b45bbc4a7ba7a6bd36aca23f662012-11-01T00:00:00Zhttp://www.dovepress.com/enhancing-microparticle-internalization-by-nonphagocytic-cells-through-a11468https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Tania Patiño, Carme Nogués, Elena Ibáñez, Leonardo BarriosUnitat de Biologia Cel lular, Departament de Biologia Cel lular, Fisiologia i Immunologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, SpainAbstract: Development of micro- and nanotechnology for the study of living cells, especially in the field of drug delivery, has gained interest in recent years. Although several studies have reported successful results in the internalization of micro- and nanoparticles in phagocytic cells, when nonphagocytic cells are used, the low internalization efficiency represents a limitation that needs to be overcome. It has been reported that covalent surface modification of micro- and nanoparticles increases their internalization rate. However, this surface modification represents an obstacle for their use as drug-delivery carriers. For this reason, the aim of the present study was to increase the capability for microparticle internalization of HeLa cells through the use of noncovalently bound transfection reagents: polyethyleneimine (PEI) Lipofectamine™ 2000 and FuGENE 6®. Both confocal microscopy and flow cytometry techniques allowed us to precisely quantify the efficiency of microparticle internalization by HeLa cells, yielding similar results. In addition, intracellular location of microparticles was analyzed through transmission electron microscopy and confocal microscopy procedures. Our results showed that free PEI at a concentration of 0.05 mM significantly increased microparticle uptake by cells, with a low cytotoxic effect. As determined by transmission electron and confocal microscopy analyses, microparticles were engulfed by plasma-membrane projections during internalization, and 24 hours later they were trapped in a lysosomal compartment. These results show the potential use of noncovalently conjugated PEI in microparticle internalization assays.Keywords: HeLa cells, internalization efficiency, endocytosis, drug deliveryPatiño TNogués CIbáñez EBarrios LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5671-5682 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Patiño T
Nogués C
Ibáñez E
Barrios L
Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine
description Tania Patiño, Carme Nogués, Elena Ibáñez, Leonardo BarriosUnitat de Biologia Cel lular, Departament de Biologia Cel lular, Fisiologia i Immunologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, SpainAbstract: Development of micro- and nanotechnology for the study of living cells, especially in the field of drug delivery, has gained interest in recent years. Although several studies have reported successful results in the internalization of micro- and nanoparticles in phagocytic cells, when nonphagocytic cells are used, the low internalization efficiency represents a limitation that needs to be overcome. It has been reported that covalent surface modification of micro- and nanoparticles increases their internalization rate. However, this surface modification represents an obstacle for their use as drug-delivery carriers. For this reason, the aim of the present study was to increase the capability for microparticle internalization of HeLa cells through the use of noncovalently bound transfection reagents: polyethyleneimine (PEI) Lipofectamine™ 2000 and FuGENE 6®. Both confocal microscopy and flow cytometry techniques allowed us to precisely quantify the efficiency of microparticle internalization by HeLa cells, yielding similar results. In addition, intracellular location of microparticles was analyzed through transmission electron microscopy and confocal microscopy procedures. Our results showed that free PEI at a concentration of 0.05 mM significantly increased microparticle uptake by cells, with a low cytotoxic effect. As determined by transmission electron and confocal microscopy analyses, microparticles were engulfed by plasma-membrane projections during internalization, and 24 hours later they were trapped in a lysosomal compartment. These results show the potential use of noncovalently conjugated PEI in microparticle internalization assays.Keywords: HeLa cells, internalization efficiency, endocytosis, drug delivery
format article
author Patiño T
Nogués C
Ibáñez E
Barrios L
author_facet Patiño T
Nogués C
Ibáñez E
Barrios L
author_sort Patiño T
title Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine
title_short Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine
title_full Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine
title_fullStr Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine
title_full_unstemmed Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine
title_sort enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/6bbc25b45bbc4a7ba7a6bd36aca23f66
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