Strategies to Mitigate Variability in Engineering Human Nasal Cartilage

Abstract Skin cancer and its associated treitments can have devastating consequences for survivors; this is particularly true when cancer occurs on the nose. Recent work has applied cell-based tissue engineering (TE) strategies to develop nasal cartilage constructs for reconstruction of the nose. In...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Stephen H. J. Andrews, Melanie Kunze, Aillette Mulet-Sierra, Lynn Williams, Khalid Ansari, Martin Osswald, Adetola B. Adesida
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6bbc303698834439b4d3ba454f32441d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6bbc303698834439b4d3ba454f32441d
record_format	dspace
spelling	oai:doaj.org-article:6bbc303698834439b4d3ba454f32441d2021-12-02T16:06:40ZStrategies to Mitigate Variability in Engineering Human Nasal Cartilage10.1038/s41598-017-06666-22045-2322https://doaj.org/article/6bbc303698834439b4d3ba454f32441d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06666-2https://doaj.org/toc/2045-2322Abstract Skin cancer and its associated treitments can have devastating consequences for survivors; this is particularly true when cancer occurs on the nose. Recent work has applied cell-based tissue engineering (TE) strategies to develop nasal cartilage constructs for reconstruction of the nose. In this study, we have generated human nasal cartilage on a clinically approved collagen scaffold to investigate the donor-to-donor variability of TE cartilage and evaluated strategies to mitigate it. We also evaluated the gene expression of the family of fibroblast growth factor receptors (FGFR1-4) and their association with tissue quality. FGFR 1 was significantly positively correlated with GAG/DNA; a measure of chondrogenic capacity. We implemented two strategies: hypoxic culture and co-culture with mesenchymal stromal cells (MSCs) to increase tissue quality. Total glycosaminoglycan (GAG) content varied significantly between donors initially, with >10–fold difference between the best and worst donor tissue. Our co-culture strategy was able to increase TE construct quality from poor quality donor tissue while supressing hypertrophy relative to MSCs alone. However, no differences were observed with the use of hypoxic culture. Tissues generated using co-culture with MSCs became vascularized and calcified in vivo, demonstrating a non-stable cartilage phenotype in co-culture and MSCs cartilage constructs.Stephen H. J. AndrewsMelanie KunzeAillette Mulet-SierraLynn WilliamsKhalid AnsariMartin OsswaldAdetola B. AdesidaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stephen H. J. Andrews Melanie Kunze Aillette Mulet-Sierra Lynn Williams Khalid Ansari Martin Osswald Adetola B. Adesida Strategies to Mitigate Variability in Engineering Human Nasal Cartilage
description	Abstract Skin cancer and its associated treitments can have devastating consequences for survivors; this is particularly true when cancer occurs on the nose. Recent work has applied cell-based tissue engineering (TE) strategies to develop nasal cartilage constructs for reconstruction of the nose. In this study, we have generated human nasal cartilage on a clinically approved collagen scaffold to investigate the donor-to-donor variability of TE cartilage and evaluated strategies to mitigate it. We also evaluated the gene expression of the family of fibroblast growth factor receptors (FGFR1-4) and their association with tissue quality. FGFR 1 was significantly positively correlated with GAG/DNA; a measure of chondrogenic capacity. We implemented two strategies: hypoxic culture and co-culture with mesenchymal stromal cells (MSCs) to increase tissue quality. Total glycosaminoglycan (GAG) content varied significantly between donors initially, with >10–fold difference between the best and worst donor tissue. Our co-culture strategy was able to increase TE construct quality from poor quality donor tissue while supressing hypertrophy relative to MSCs alone. However, no differences were observed with the use of hypoxic culture. Tissues generated using co-culture with MSCs became vascularized and calcified in vivo, demonstrating a non-stable cartilage phenotype in co-culture and MSCs cartilage constructs.
format	article
author	Stephen H. J. Andrews Melanie Kunze Aillette Mulet-Sierra Lynn Williams Khalid Ansari Martin Osswald Adetola B. Adesida
author_facet	Stephen H. J. Andrews Melanie Kunze Aillette Mulet-Sierra Lynn Williams Khalid Ansari Martin Osswald Adetola B. Adesida
author_sort	Stephen H. J. Andrews
title	Strategies to Mitigate Variability in Engineering Human Nasal Cartilage
title_short	Strategies to Mitigate Variability in Engineering Human Nasal Cartilage
title_full	Strategies to Mitigate Variability in Engineering Human Nasal Cartilage
title_fullStr	Strategies to Mitigate Variability in Engineering Human Nasal Cartilage
title_full_unstemmed	Strategies to Mitigate Variability in Engineering Human Nasal Cartilage
title_sort	strategies to mitigate variability in engineering human nasal cartilage
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6bbc303698834439b4d3ba454f32441d
work_keys_str_mv	AT stephenhjandrews strategiestomitigatevariabilityinengineeringhumannasalcartilage AT melaniekunze strategiestomitigatevariabilityinengineeringhumannasalcartilage AT aillettemuletsierra strategiestomitigatevariabilityinengineeringhumannasalcartilage AT lynnwilliams strategiestomitigatevariabilityinengineeringhumannasalcartilage AT khalidansari strategiestomitigatevariabilityinengineeringhumannasalcartilage AT martinosswald strategiestomitigatevariabilityinengineeringhumannasalcartilage AT adetolabadesida strategiestomitigatevariabilityinengineeringhumannasalcartilage
_version_	1718384907376394240

Strategies to Mitigate Variability in Engineering Human Nasal Cartilage

Ejemplares similares