Contrasting effects of singlet oxygen and hydrogen peroxide on bacterial community composition in a humic lake.

Light excitation of humic matter generates reactive oxygen species (ROS) in surface waters of aquatic ecosystems. Abundant ROS generated in humic matter rich lakes include singlet oxygen ((1)O2) and hydrogen peroxide (H2O2). Because these ROS differ in half-life time and toxicity, we compared their...

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Autores principales:	Stefanie P Glaeser, Bork A Berghoff, Verena Stratmann, Hans-Peter Grossart, Jens Glaeser
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6bc28f9315404ce19cedd7af116c404f
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spelling	oai:doaj.org-article:6bc28f9315404ce19cedd7af116c404f2021-11-18T08:26:20ZContrasting effects of singlet oxygen and hydrogen peroxide on bacterial community composition in a humic lake.1932-620310.1371/journal.pone.0092518https://doaj.org/article/6bc28f9315404ce19cedd7af116c404f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24667441/?tool=EBIhttps://doaj.org/toc/1932-6203Light excitation of humic matter generates reactive oxygen species (ROS) in surface waters of aquatic ecosystems. Abundant ROS generated in humic matter rich lakes include singlet oxygen ((1)O2) and hydrogen peroxide (H2O2). Because these ROS differ in half-life time and toxicity, we compared their effects on microbial activity ((14)C-Leucine incorporation) and bacterial community composition (BCC) in surface waters of humic Lake Grosse Fuchskuhle (North-eastern Germany). For this purpose, experiments with water samples collected from the lake were conducted in July 2006, September 2008 and August 2009. Artificially increased (1)O2 and H2O2 concentrations inhibited microbial activity in water samples to a similar extent, but the effect of the respective ROS on BCC varied strongly. BCC analysis by 16S rRNA gene clone libraries and RT-PCR DGGE revealed ROS specific changes in relative abundance and activity of major bacterial groups and composition of dominating phylotypes. These changes were consistent in the three experiments performed in different years. The relative abundance of Polynucleobacter necessarius, Limnohabitans-related phylotypes (Betaproteobacteria), and Novosphingobium acidiphilum (Alphaproteobacteria) increased or was not affected by photo-sensitized (1)O2 exposure, but decreased after H2O2 exposure. The opposite pattern was found for Actinobacteria of the freshwater AcI-B cluster which were highly sensitive to (1)O2 but not to H2O2 exposure. Furthermore, group-specific RT-PCR DGGE analysis revealed that particle-attached P. necessarius and Limnohabitans-related phylotypes exhibit higher resistance to (1)O2 exposure compared to free-living populations. These results imply that (1)O2 acts as a factor in niche separation of closely affiliated Polynucleobacter and Limnohabitans-related phylotypes. Consequently, oxidative stress caused by photochemical ROS generation should be regarded as an environmental variable determining abundance, activity, and phylotype composition of environmentally relevant bacterial groups, in particular in illuminated and humic matter rich waters.Stefanie P GlaeserBork A BerghoffVerena StratmannHans-Peter GrossartJens GlaeserPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92518 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefanie P Glaeser Bork A Berghoff Verena Stratmann Hans-Peter Grossart Jens Glaeser Contrasting effects of singlet oxygen and hydrogen peroxide on bacterial community composition in a humic lake.
description	Light excitation of humic matter generates reactive oxygen species (ROS) in surface waters of aquatic ecosystems. Abundant ROS generated in humic matter rich lakes include singlet oxygen ((1)O2) and hydrogen peroxide (H2O2). Because these ROS differ in half-life time and toxicity, we compared their effects on microbial activity ((14)C-Leucine incorporation) and bacterial community composition (BCC) in surface waters of humic Lake Grosse Fuchskuhle (North-eastern Germany). For this purpose, experiments with water samples collected from the lake were conducted in July 2006, September 2008 and August 2009. Artificially increased (1)O2 and H2O2 concentrations inhibited microbial activity in water samples to a similar extent, but the effect of the respective ROS on BCC varied strongly. BCC analysis by 16S rRNA gene clone libraries and RT-PCR DGGE revealed ROS specific changes in relative abundance and activity of major bacterial groups and composition of dominating phylotypes. These changes were consistent in the three experiments performed in different years. The relative abundance of Polynucleobacter necessarius, Limnohabitans-related phylotypes (Betaproteobacteria), and Novosphingobium acidiphilum (Alphaproteobacteria) increased or was not affected by photo-sensitized (1)O2 exposure, but decreased after H2O2 exposure. The opposite pattern was found for Actinobacteria of the freshwater AcI-B cluster which were highly sensitive to (1)O2 but not to H2O2 exposure. Furthermore, group-specific RT-PCR DGGE analysis revealed that particle-attached P. necessarius and Limnohabitans-related phylotypes exhibit higher resistance to (1)O2 exposure compared to free-living populations. These results imply that (1)O2 acts as a factor in niche separation of closely affiliated Polynucleobacter and Limnohabitans-related phylotypes. Consequently, oxidative stress caused by photochemical ROS generation should be regarded as an environmental variable determining abundance, activity, and phylotype composition of environmentally relevant bacterial groups, in particular in illuminated and humic matter rich waters.
format	article
author	Stefanie P Glaeser Bork A Berghoff Verena Stratmann Hans-Peter Grossart Jens Glaeser
author_facet	Stefanie P Glaeser Bork A Berghoff Verena Stratmann Hans-Peter Grossart Jens Glaeser
author_sort	Stefanie P Glaeser
title	Contrasting effects of singlet oxygen and hydrogen peroxide on bacterial community composition in a humic lake.
title_short	Contrasting effects of singlet oxygen and hydrogen peroxide on bacterial community composition in a humic lake.
title_full	Contrasting effects of singlet oxygen and hydrogen peroxide on bacterial community composition in a humic lake.
title_fullStr	Contrasting effects of singlet oxygen and hydrogen peroxide on bacterial community composition in a humic lake.
title_full_unstemmed	Contrasting effects of singlet oxygen and hydrogen peroxide on bacterial community composition in a humic lake.
title_sort	contrasting effects of singlet oxygen and hydrogen peroxide on bacterial community composition in a humic lake.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/6bc28f9315404ce19cedd7af116c404f
work_keys_str_mv	AT stefaniepglaeser contrastingeffectsofsingletoxygenandhydrogenperoxideonbacterialcommunitycompositioninahumiclake AT borkaberghoff contrastingeffectsofsingletoxygenandhydrogenperoxideonbacterialcommunitycompositioninahumiclake AT verenastratmann contrastingeffectsofsingletoxygenandhydrogenperoxideonbacterialcommunitycompositioninahumiclake AT hanspetergrossart contrastingeffectsofsingletoxygenandhydrogenperoxideonbacterialcommunitycompositioninahumiclake AT jensglaeser contrastingeffectsofsingletoxygenandhydrogenperoxideonbacterialcommunitycompositioninahumiclake
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Contrasting effects of singlet oxygen and hydrogen peroxide on bacterial community composition in a humic lake.

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