RNA expression and risk of venous thromboembolism in lung cancer

Abstract Background The propensity to develop venous thromboembolism (VTE) on the basis of individual tumor biological features remains unknown. Objectives We conducted a whole transcriptome RNA sequencing strategy, focusing on a single cancer type (lung cancer), to identify biomarkers of cancer‐ass...

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Autores principales:	Tamara A. Sussman, Mohamed E. Abazeed, Keith R. McCrae, Alok A. Khorana
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2020
Materias:	biomarkers gene expression inflammation lung neoplasms sequence analysis RNA Diseases of the blood and blood-forming organs RC633-647.5
Acceso en línea:	https://doaj.org/article/6bc97f724f464bf9b4446adc621c394f
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spelling	oai:doaj.org-article:6bc97f724f464bf9b4446adc621c394f2021-11-15T06:10:44ZRNA expression and risk of venous thromboembolism in lung cancer2475-037910.1002/rth2.12284https://doaj.org/article/6bc97f724f464bf9b4446adc621c394f2020-01-01T00:00:00Zhttps://doi.org/10.1002/rth2.12284https://doaj.org/toc/2475-0379Abstract Background The propensity to develop venous thromboembolism (VTE) on the basis of individual tumor biological features remains unknown. Objectives We conducted a whole transcriptome RNA sequencing strategy, focusing on a single cancer type (lung cancer), to identify biomarkers of cancer‐associated VTE. Methods Twelve propensity‐matched patients, 6 each with or without VTE, were identified from a prospective institutional review board–approved registry at the Cleveland Clinic with available tissue from surgical excision of a primary lung mass between 2010 and 2015. Patients were propensity matched based on age, sex, race, history of prior cancer, date of cancer diagnosis, stage, histology, number of lines of chemotherapy, and length of follow‐up. RNA sequencing was performed on tumor tissue, and gene set enrichment analysis (GSEA) was performed on differentially expressed genes. Results We identified 1037 genes with differential expression. In patients with VTE, 869 genes were overexpressed and 168 were underexpressed compared to patients without VTE. Of these, 276 overexpressed and 35 underexpressed were significantly different (Q < 0.05). GSEA revealed upregulation of genes in complement, inflammation, and KRAS signaling pathways in tumors from patients with VTE. Conclusions These differentially expressed genes and associated pathways provide biologic insights into cancer‐associated VTE and may provide insignts to develop new risk stratification schemes, prevention, or treatment strategies.Tamara A. SussmanMohamed E. AbazeedKeith R. McCraeAlok A. KhoranaWileyarticlebiomarkersgene expressioninflammationlung neoplasmssequence analysisRNADiseases of the blood and blood-forming organsRC633-647.5ENResearch and Practice in Thrombosis and Haemostasis, Vol 4, Iss 1, Pp 117-123 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	biomarkers gene expression inflammation lung neoplasms sequence analysis RNA Diseases of the blood and blood-forming organs RC633-647.5
spellingShingle	biomarkers gene expression inflammation lung neoplasms sequence analysis RNA Diseases of the blood and blood-forming organs RC633-647.5 Tamara A. Sussman Mohamed E. Abazeed Keith R. McCrae Alok A. Khorana RNA expression and risk of venous thromboembolism in lung cancer
description	Abstract Background The propensity to develop venous thromboembolism (VTE) on the basis of individual tumor biological features remains unknown. Objectives We conducted a whole transcriptome RNA sequencing strategy, focusing on a single cancer type (lung cancer), to identify biomarkers of cancer‐associated VTE. Methods Twelve propensity‐matched patients, 6 each with or without VTE, were identified from a prospective institutional review board–approved registry at the Cleveland Clinic with available tissue from surgical excision of a primary lung mass between 2010 and 2015. Patients were propensity matched based on age, sex, race, history of prior cancer, date of cancer diagnosis, stage, histology, number of lines of chemotherapy, and length of follow‐up. RNA sequencing was performed on tumor tissue, and gene set enrichment analysis (GSEA) was performed on differentially expressed genes. Results We identified 1037 genes with differential expression. In patients with VTE, 869 genes were overexpressed and 168 were underexpressed compared to patients without VTE. Of these, 276 overexpressed and 35 underexpressed were significantly different (Q < 0.05). GSEA revealed upregulation of genes in complement, inflammation, and KRAS signaling pathways in tumors from patients with VTE. Conclusions These differentially expressed genes and associated pathways provide biologic insights into cancer‐associated VTE and may provide insignts to develop new risk stratification schemes, prevention, or treatment strategies.
format	article
author	Tamara A. Sussman Mohamed E. Abazeed Keith R. McCrae Alok A. Khorana
author_facet	Tamara A. Sussman Mohamed E. Abazeed Keith R. McCrae Alok A. Khorana
author_sort	Tamara A. Sussman
title	RNA expression and risk of venous thromboembolism in lung cancer
title_short	RNA expression and risk of venous thromboembolism in lung cancer
title_full	RNA expression and risk of venous thromboembolism in lung cancer
title_fullStr	RNA expression and risk of venous thromboembolism in lung cancer
title_full_unstemmed	RNA expression and risk of venous thromboembolism in lung cancer
title_sort	rna expression and risk of venous thromboembolism in lung cancer
publisher	Wiley
publishDate	2020
url	https://doaj.org/article/6bc97f724f464bf9b4446adc621c394f
work_keys_str_mv	AT tamaraasussman rnaexpressionandriskofvenousthromboembolisminlungcancer AT mohamedeabazeed rnaexpressionandriskofvenousthromboembolisminlungcancer AT keithrmccrae rnaexpressionandriskofvenousthromboembolisminlungcancer AT alokakhorana rnaexpressionandriskofvenousthromboembolisminlungcancer
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RNA expression and risk of venous thromboembolism in lung cancer

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