EFFICIENCY OF RECOMBINANT TNF-BINDING PROTEIN FROM VARIOLA VIRUS IN A MODEL OF COLLAGEN-INDUCED ARTHRITIS

Abstract. This paper presents the results of the research on the effectiveness of recombinant TNF-binding protein of variola virus (VARV-CrmB) in a model of collagen-induced arthritis (CIA) in mice (CBAxC57Bl6) F1. The introduction of VARV-CrmB and polyclonal antibody to recombinant mouse TNF (poly-...

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Autores principales:	D. D. Tsyrendorzhiev, S. V. Sennikov, I. A. Orlovskaya, I. P. Gileva, I. A. Ryazankin, L. B. Toporkova, V. V. Kurilin, Yu. A. Lopatnikova, A. A. Grydina, S. N. Schelkunov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	tumor necrosis factor collagen variola virus leukocytes rheumatoid arthritis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/6bd0fc6fdfea43959facbd28c55ebf8b
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spelling	oai:doaj.org-article:6bd0fc6fdfea43959facbd28c55ebf8b2021-11-18T08:03:43ZEFFICIENCY OF RECOMBINANT TNF-BINDING PROTEIN FROM VARIOLA VIRUS IN A MODEL OF COLLAGEN-INDUCED ARTHRITIS1563-06252313-741X10.15789/1563-0625-2013-6-513-524https://doaj.org/article/6bd0fc6fdfea43959facbd28c55ebf8b2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/675https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. This paper presents the results of the research on the effectiveness of recombinant TNF-binding protein of variola virus (VARV-CrmB) in a model of collagen-induced arthritis (CIA) in mice (CBAxC57Bl6) F1. The introduction of VARV-CrmB and polyclonal antibody to recombinant mouse TNF (poly-AbMuTNF) led to an improvement of clinical manifestations of CIA by reducing the swelling and increasing the mobility of mice limbs. The introduction of VARV-CrmB and poly-AbMuTNF reduced the number of neutrophilic granulocytes and granulocytic precursors. The introduction of VARV-CrmB and poly-AbMuTNF into mice decreased collagenolysis in the blood serum and the content of glycosaminoglycans at the early stages of experimentation. Treatment with VARV-CrmB and poly-AbMuTNF of mice with CIA significantly decreased the chemiluminescence response of blood leukocytes. VARV-CrmB exerted more pronounced inhibitory effect on the production of reactive oxygen metabolites by blood leukocytes of mice with CIA than poly-AbMuTNF. Improvement of clinical condition of the mice with CIA has a more prolonged effect following introduction of the VARV-CrmB than after injection of poly-AbMuTNF. The results suggest the recombinant viral protein VARVCrmB to be a new potential TNF-antagonist.D. D. TsyrendorzhievS. V. SennikovI. A. OrlovskayaI. P. GilevaI. A. RyazankinL. B. ToporkovaV. V. KurilinYu. A. LopatnikovaA. A. GrydinaS. N. SchelkunovSPb RAACIarticletumor necrosis factorcollagenvariola virusleukocytesrheumatoid arthritisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 15, Iss 6, Pp 513-524 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	tumor necrosis factor collagen variola virus leukocytes rheumatoid arthritis Immunologic diseases. Allergy RC581-607
spellingShingle	tumor necrosis factor collagen variola virus leukocytes rheumatoid arthritis Immunologic diseases. Allergy RC581-607 D. D. Tsyrendorzhiev S. V. Sennikov I. A. Orlovskaya I. P. Gileva I. A. Ryazankin L. B. Toporkova V. V. Kurilin Yu. A. Lopatnikova A. A. Grydina S. N. Schelkunov EFFICIENCY OF RECOMBINANT TNF-BINDING PROTEIN FROM VARIOLA VIRUS IN A MODEL OF COLLAGEN-INDUCED ARTHRITIS
description	Abstract. This paper presents the results of the research on the effectiveness of recombinant TNF-binding protein of variola virus (VARV-CrmB) in a model of collagen-induced arthritis (CIA) in mice (CBAxC57Bl6) F1. The introduction of VARV-CrmB and polyclonal antibody to recombinant mouse TNF (poly-AbMuTNF) led to an improvement of clinical manifestations of CIA by reducing the swelling and increasing the mobility of mice limbs. The introduction of VARV-CrmB and poly-AbMuTNF reduced the number of neutrophilic granulocytes and granulocytic precursors. The introduction of VARV-CrmB and poly-AbMuTNF into mice decreased collagenolysis in the blood serum and the content of glycosaminoglycans at the early stages of experimentation. Treatment with VARV-CrmB and poly-AbMuTNF of mice with CIA significantly decreased the chemiluminescence response of blood leukocytes. VARV-CrmB exerted more pronounced inhibitory effect on the production of reactive oxygen metabolites by blood leukocytes of mice with CIA than poly-AbMuTNF. Improvement of clinical condition of the mice with CIA has a more prolonged effect following introduction of the VARV-CrmB than after injection of poly-AbMuTNF. The results suggest the recombinant viral protein VARVCrmB to be a new potential TNF-antagonist.
format	article
author	D. D. Tsyrendorzhiev S. V. Sennikov I. A. Orlovskaya I. P. Gileva I. A. Ryazankin L. B. Toporkova V. V. Kurilin Yu. A. Lopatnikova A. A. Grydina S. N. Schelkunov
author_facet	D. D. Tsyrendorzhiev S. V. Sennikov I. A. Orlovskaya I. P. Gileva I. A. Ryazankin L. B. Toporkova V. V. Kurilin Yu. A. Lopatnikova A. A. Grydina S. N. Schelkunov
author_sort	D. D. Tsyrendorzhiev
title	EFFICIENCY OF RECOMBINANT TNF-BINDING PROTEIN FROM VARIOLA VIRUS IN A MODEL OF COLLAGEN-INDUCED ARTHRITIS
title_short	EFFICIENCY OF RECOMBINANT TNF-BINDING PROTEIN FROM VARIOLA VIRUS IN A MODEL OF COLLAGEN-INDUCED ARTHRITIS
title_full	EFFICIENCY OF RECOMBINANT TNF-BINDING PROTEIN FROM VARIOLA VIRUS IN A MODEL OF COLLAGEN-INDUCED ARTHRITIS
title_fullStr	EFFICIENCY OF RECOMBINANT TNF-BINDING PROTEIN FROM VARIOLA VIRUS IN A MODEL OF COLLAGEN-INDUCED ARTHRITIS
title_full_unstemmed	EFFICIENCY OF RECOMBINANT TNF-BINDING PROTEIN FROM VARIOLA VIRUS IN A MODEL OF COLLAGEN-INDUCED ARTHRITIS
title_sort	efficiency of recombinant tnf-binding protein from variola virus in a model of collagen-induced arthritis
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/6bd0fc6fdfea43959facbd28c55ebf8b
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EFFICIENCY OF RECOMBINANT TNF-BINDING PROTEIN FROM VARIOLA VIRUS IN A MODEL OF COLLAGEN-INDUCED ARTHRITIS

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