DIAGNOSTIC ACCURACY OF IN-HOUSE BIOCHEMICAL TESTING FOR IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM VARIOUS CLINICAL SPECIMENSAGAINST VITEK 2 SYSTEM

Objective: To determine the diagnostic accuracy of in-house biochemical testing for identification of enterococcus species isolated from various clinical specimens against gold standard i.e., automated Vitek 2 system. This study also includes the antimicrobial susceptibility testing of enterococci a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mariam Sarwar, Syed Adeel Hussain Gardezi, Gohar Zaman, Aamer Ikram, Wajid Hussain, Muhammad Tahir Khadim
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2021
Materias:	enterococcus spp in-house biochemical testing vitek 2 system Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6bd2b198851748a8a4529c83c806d538
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6bd2b198851748a8a4529c83c806d538
record_format	dspace
spelling	oai:doaj.org-article:6bd2b198851748a8a4529c83c806d5382021-12-02T18:01:28ZDIAGNOSTIC ACCURACY OF IN-HOUSE BIOCHEMICAL TESTING FOR IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM VARIOUS CLINICAL SPECIMENSAGAINST VITEK 2 SYSTEM0030-96482411-884210.51253/pafmj.v71i1.3095https://doaj.org/article/6bd2b198851748a8a4529c83c806d5382021-02-01T00:00:00Zhttps://pafmj.org/index.php/PAFMJ/article/view/3095https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To determine the diagnostic accuracy of in-house biochemical testing for identification of enterococcus species isolated from various clinical specimens against gold standard i.e., automated Vitek 2 system. This study also includes the antimicrobial susceptibility testing of enterococci against various antimicrobials. Study Design: Cross-sectional comparative study. Place and Duration of Study: Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi, from Apr 2017 to Mar 2018. Methodology: A total of 218 isolates from various clinical specimens suspected to be Enterococcus spp. were checked by in-house biochemical testing including bile esculin, 6.5% NaCl and 1% arabinose and results were compared with Vitek 2 compact system. The frequencies were determined by both systems and antimicrobial susceptibility testing was performed by disk diffusion as per clinical and laboratory standards institute guidelines. Results: Comparing the results of in-house testing with gold standard i.e., Vitek 2 system, the statistical data was calculated. Sensitivity turned out to be 100%, Specificity was found to be 68.75%. Positive and negative predictive values were 97.58% and 100% respectively. Accuracy turned out to be 97.71%. Conclusion: The in-house biochemical testing can be quite a useful method for identification of enterococci in resource-limited settings. However, it requires overight incubation and cannot identify other enterococcal species and non-enterococcal species. Vitek 2 is an automated system that is easy-to-handle, provides a rapid and reasonably accurate identification of enterococci alongwith accurate AST results. Enterococcal isolates from various clinical specimens in our setup showed least resistance to linezolid, followed by teicoplanin and vancomycin. Nitrofurantoin and.............Mariam SarwarSyed Adeel Hussain GardeziGohar ZamanAamer IkramWajid HussainMuhammad Tahir KhadimArmy Medical College Rawalpindiarticleenterococcus sppin-house biochemical testingvitek 2 systemMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 71, Iss 1, Pp 155-61 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	enterococcus spp in-house biochemical testing vitek 2 system Medicine R Medicine (General) R5-920
spellingShingle	enterococcus spp in-house biochemical testing vitek 2 system Medicine R Medicine (General) R5-920 Mariam Sarwar Syed Adeel Hussain Gardezi Gohar Zaman Aamer Ikram Wajid Hussain Muhammad Tahir Khadim DIAGNOSTIC ACCURACY OF IN-HOUSE BIOCHEMICAL TESTING FOR IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM VARIOUS CLINICAL SPECIMENSAGAINST VITEK 2 SYSTEM
description	Objective: To determine the diagnostic accuracy of in-house biochemical testing for identification of enterococcus species isolated from various clinical specimens against gold standard i.e., automated Vitek 2 system. This study also includes the antimicrobial susceptibility testing of enterococci against various antimicrobials. Study Design: Cross-sectional comparative study. Place and Duration of Study: Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi, from Apr 2017 to Mar 2018. Methodology: A total of 218 isolates from various clinical specimens suspected to be Enterococcus spp. were checked by in-house biochemical testing including bile esculin, 6.5% NaCl and 1% arabinose and results were compared with Vitek 2 compact system. The frequencies were determined by both systems and antimicrobial susceptibility testing was performed by disk diffusion as per clinical and laboratory standards institute guidelines. Results: Comparing the results of in-house testing with gold standard i.e., Vitek 2 system, the statistical data was calculated. Sensitivity turned out to be 100%, Specificity was found to be 68.75%. Positive and negative predictive values were 97.58% and 100% respectively. Accuracy turned out to be 97.71%. Conclusion: The in-house biochemical testing can be quite a useful method for identification of enterococci in resource-limited settings. However, it requires overight incubation and cannot identify other enterococcal species and non-enterococcal species. Vitek 2 is an automated system that is easy-to-handle, provides a rapid and reasonably accurate identification of enterococci alongwith accurate AST results. Enterococcal isolates from various clinical specimens in our setup showed least resistance to linezolid, followed by teicoplanin and vancomycin. Nitrofurantoin and.............
format	article
author	Mariam Sarwar Syed Adeel Hussain Gardezi Gohar Zaman Aamer Ikram Wajid Hussain Muhammad Tahir Khadim
author_facet	Mariam Sarwar Syed Adeel Hussain Gardezi Gohar Zaman Aamer Ikram Wajid Hussain Muhammad Tahir Khadim
author_sort	Mariam Sarwar
title	DIAGNOSTIC ACCURACY OF IN-HOUSE BIOCHEMICAL TESTING FOR IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM VARIOUS CLINICAL SPECIMENSAGAINST VITEK 2 SYSTEM
title_short	DIAGNOSTIC ACCURACY OF IN-HOUSE BIOCHEMICAL TESTING FOR IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM VARIOUS CLINICAL SPECIMENSAGAINST VITEK 2 SYSTEM
title_full	DIAGNOSTIC ACCURACY OF IN-HOUSE BIOCHEMICAL TESTING FOR IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM VARIOUS CLINICAL SPECIMENSAGAINST VITEK 2 SYSTEM
title_fullStr	DIAGNOSTIC ACCURACY OF IN-HOUSE BIOCHEMICAL TESTING FOR IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM VARIOUS CLINICAL SPECIMENSAGAINST VITEK 2 SYSTEM
title_full_unstemmed	DIAGNOSTIC ACCURACY OF IN-HOUSE BIOCHEMICAL TESTING FOR IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM VARIOUS CLINICAL SPECIMENSAGAINST VITEK 2 SYSTEM
title_sort	diagnostic accuracy of in-house biochemical testing for identification of enterococcus species isolated from various clinical specimensagainst vitek 2 system
publisher	Army Medical College Rawalpindi
publishDate	2021
url	https://doaj.org/article/6bd2b198851748a8a4529c83c806d538
work_keys_str_mv	AT mariamsarwar diagnosticaccuracyofinhousebiochemicaltestingforidentificationofenterococcusspeciesisolatedfromvariousclinicalspecimensagainstvitek2system AT syedadeelhussaingardezi diagnosticaccuracyofinhousebiochemicaltestingforidentificationofenterococcusspeciesisolatedfromvariousclinicalspecimensagainstvitek2system AT goharzaman diagnosticaccuracyofinhousebiochemicaltestingforidentificationofenterococcusspeciesisolatedfromvariousclinicalspecimensagainstvitek2system AT aamerikram diagnosticaccuracyofinhousebiochemicaltestingforidentificationofenterococcusspeciesisolatedfromvariousclinicalspecimensagainstvitek2system AT wajidhussain diagnosticaccuracyofinhousebiochemicaltestingforidentificationofenterococcusspeciesisolatedfromvariousclinicalspecimensagainstvitek2system AT muhammadtahirkhadim diagnosticaccuracyofinhousebiochemicaltestingforidentificationofenterococcusspeciesisolatedfromvariousclinicalspecimensagainstvitek2system
_version_	1718378994707988480

DIAGNOSTIC ACCURACY OF IN-HOUSE BIOCHEMICAL TESTING FOR IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM VARIOUS CLINICAL SPECIMENSAGAINST VITEK 2 SYSTEM

Ejemplares similares