Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract

Medicinal plants are used as an important source of medicines in pharmaceutical industry. Rubia cordifolia is widely used to cure diabetes mellitus. Present study was aimed to investigate the antihyperglycemic effects of different fractions of R. cordifolia leaves and to analyze its antioxidant effe...

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Autores principales:	Khan Muhammad Shafiq, Aziz Shahid, Khan Muhammad Zakryya, Khalid Zafar Mahmood, Riaz Muhammad, Ahmed Dawood, Ali Iftikhar, Arif Nazia, Wang Deiji, Ahmad Muhammad Sheeraz, Zahid Nafeesa
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	diabetes mellitus polyphenolic compounds alloxan pancreas rubia cordifolia antioxidant Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/6bd2bcc36f424e218aa0d94bc8612e2c
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spelling	oai:doaj.org-article:6bd2bcc36f424e218aa0d94bc8612e2c2021-12-05T14:10:44ZAntihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract2391-542010.1515/chem-2021-0053https://doaj.org/article/6bd2bcc36f424e218aa0d94bc8612e2c2021-05-01T00:00:00Zhttps://doi.org/10.1515/chem-2021-0053https://doaj.org/toc/2391-5420Medicinal plants are used as an important source of medicines in pharmaceutical industry. Rubia cordifolia is widely used to cure diabetes mellitus. Present study was aimed to investigate the antihyperglycemic effects of different fractions of R. cordifolia leaves and to analyze its antioxidant effect and phytochemical composition. Male albino mice were randomly distributed into seven groups (n = 7). Group-I was normal control, group-II was Alloxan (100 mg/kg)-induced diabetic control, and group-III was standard drug (Glibenclamide 0.5 mg/kg)-treated group. Animals in groups IV–VII were treated with n-hexane fraction, ethyl acetate fraction, n-butanol fraction and aqueous fraction of R. cordifolia, orally administered (100 mg/kg) once daily up to 28 days after Alloxan induction, respectively. Methanolic extract (ME) and fractions of R. cordifilia were analyzed for antioxidant activity and quantification of total phenolic content and total flavonoid content. HPLC of ME and most active fractions were performed. The results showed that RCEF (G-V) and RCBF (G-VI) have significantly (P < 0.05) reduced the increased level of glucose as compared to toxicant control group. It was further revealed that EF and BF have higher antioxidant activity (having IC50 34.9, 36.86 (µg/mL)) owing to phenolic and flavonoid identified by HPLC.Khan Muhammad ShafiqAziz ShahidKhan Muhammad ZakryyaKhalid Zafar MahmoodRiaz MuhammadAhmed DawoodAli IftikharArif NaziaWang DeijiAhmad Muhammad SheerazZahid NafeesaDe Gruyterarticlediabetes mellituspolyphenolic compoundsalloxanpancreasrubia cordifoliaantioxidantChemistryQD1-999ENOpen Chemistry, Vol 19, Iss 1, Pp 586-599 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetes mellitus polyphenolic compounds alloxan pancreas rubia cordifolia antioxidant Chemistry QD1-999
spellingShingle	diabetes mellitus polyphenolic compounds alloxan pancreas rubia cordifolia antioxidant Chemistry QD1-999 Khan Muhammad Shafiq Aziz Shahid Khan Muhammad Zakryya Khalid Zafar Mahmood Riaz Muhammad Ahmed Dawood Ali Iftikhar Arif Nazia Wang Deiji Ahmad Muhammad Sheeraz Zahid Nafeesa Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract
description	Medicinal plants are used as an important source of medicines in pharmaceutical industry. Rubia cordifolia is widely used to cure diabetes mellitus. Present study was aimed to investigate the antihyperglycemic effects of different fractions of R. cordifolia leaves and to analyze its antioxidant effect and phytochemical composition. Male albino mice were randomly distributed into seven groups (n = 7). Group-I was normal control, group-II was Alloxan (100 mg/kg)-induced diabetic control, and group-III was standard drug (Glibenclamide 0.5 mg/kg)-treated group. Animals in groups IV–VII were treated with n-hexane fraction, ethyl acetate fraction, n-butanol fraction and aqueous fraction of R. cordifolia, orally administered (100 mg/kg) once daily up to 28 days after Alloxan induction, respectively. Methanolic extract (ME) and fractions of R. cordifilia were analyzed for antioxidant activity and quantification of total phenolic content and total flavonoid content. HPLC of ME and most active fractions were performed. The results showed that RCEF (G-V) and RCBF (G-VI) have significantly (P < 0.05) reduced the increased level of glucose as compared to toxicant control group. It was further revealed that EF and BF have higher antioxidant activity (having IC50 34.9, 36.86 (µg/mL)) owing to phenolic and flavonoid identified by HPLC.
format	article
author	Khan Muhammad Shafiq Aziz Shahid Khan Muhammad Zakryya Khalid Zafar Mahmood Riaz Muhammad Ahmed Dawood Ali Iftikhar Arif Nazia Wang Deiji Ahmad Muhammad Sheeraz Zahid Nafeesa
author_facet	Khan Muhammad Shafiq Aziz Shahid Khan Muhammad Zakryya Khalid Zafar Mahmood Riaz Muhammad Ahmed Dawood Ali Iftikhar Arif Nazia Wang Deiji Ahmad Muhammad Sheeraz Zahid Nafeesa
author_sort	Khan Muhammad Shafiq
title	Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract
title_short	Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract
title_full	Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract
title_fullStr	Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract
title_full_unstemmed	Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract
title_sort	antihyperglycemic effect and phytochemical investigation of rubia cordifolia (indian madder) leaves extract
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/6bd2bcc36f424e218aa0d94bc8612e2c
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Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract

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