Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Existing methods for nanoscale visualization of biological targets in thick samples require complex hardware. Here, the authors combine the standard spinning disk confocal (SDC) microscopy with DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) to image proteins, DNA and RNA dee...

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Autores principales:	Florian Schueder, Juanita Lara-Gutiérrez, Brian J. Beliveau, Sinem K. Saka, Hiroshi M. Sasaki, Johannes B. Woehrstein, Maximilian T. Strauss, Heinrich Grabmayr, Peng Yin, Ralf Jungmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6bd8e3c1748a4c7081ee96531335338f
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spelling	oai:doaj.org-article:6bd8e3c1748a4c7081ee96531335338f2021-12-02T15:38:45ZMultiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT10.1038/s41467-017-02028-82041-1723https://doaj.org/article/6bd8e3c1748a4c7081ee96531335338f2017-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-02028-8https://doaj.org/toc/2041-1723Existing methods for nanoscale visualization of biological targets in thick samples require complex hardware. Here, the authors combine the standard spinning disk confocal (SDC) microscopy with DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) to image proteins, DNA and RNA deep in cells.Florian SchuederJuanita Lara-GutiérrezBrian J. BeliveauSinem K. SakaHiroshi M. SasakiJohannes B. WoehrsteinMaximilian T. StraussHeinrich GrabmayrPeng YinRalf JungmannNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Florian Schueder Juanita Lara-Gutiérrez Brian J. Beliveau Sinem K. Saka Hiroshi M. Sasaki Johannes B. Woehrstein Maximilian T. Strauss Heinrich Grabmayr Peng Yin Ralf Jungmann Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT
description	Existing methods for nanoscale visualization of biological targets in thick samples require complex hardware. Here, the authors combine the standard spinning disk confocal (SDC) microscopy with DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) to image proteins, DNA and RNA deep in cells.
format	article
author	Florian Schueder Juanita Lara-Gutiérrez Brian J. Beliveau Sinem K. Saka Hiroshi M. Sasaki Johannes B. Woehrstein Maximilian T. Strauss Heinrich Grabmayr Peng Yin Ralf Jungmann
author_facet	Florian Schueder Juanita Lara-Gutiérrez Brian J. Beliveau Sinem K. Saka Hiroshi M. Sasaki Johannes B. Woehrstein Maximilian T. Strauss Heinrich Grabmayr Peng Yin Ralf Jungmann
author_sort	Florian Schueder
title	Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT
title_short	Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT
title_full	Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT
title_fullStr	Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT
title_full_unstemmed	Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT
title_sort	multiplexed 3d super-resolution imaging of whole cells using spinning disk confocal microscopy and dna-paint
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6bd8e3c1748a4c7081ee96531335338f
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Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

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