Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Existing methods for nanoscale visualization of biological targets in thick samples require complex hardware. Here, the authors combine the standard spinning disk confocal (SDC) microscopy with DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) to image proteins, DNA and RNA dee...

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Autores principales: Florian Schueder, Juanita Lara-Gutiérrez, Brian J. Beliveau, Sinem K. Saka, Hiroshi M. Sasaki, Johannes B. Woehrstein, Maximilian T. Strauss, Heinrich Grabmayr, Peng Yin, Ralf Jungmann
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Science
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Acceso en línea:https://doaj.org/article/6bd8e3c1748a4c7081ee96531335338f
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