A novel bivalent chromatin associates with rapid induction of camalexin biosynthesis genes in response to a pathogen signal in Arabidopsis
Temporal dynamics of gene expression underpin responses to internal and environmental stimuli. In eukaryotes, regulation of gene induction includes changing chromatin states at target genes and recruiting the transcriptional machinery that includes transcription factors. As one of the most potent de...
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eLife Sciences Publications Ltd
2021
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oai:doaj.org-article:6be1e85e972c4bdbbf99d4881d98b0a42021-11-29T12:14:07ZA novel bivalent chromatin associates with rapid induction of camalexin biosynthesis genes in response to a pathogen signal in Arabidopsis10.7554/eLife.695082050-084Xe69508https://doaj.org/article/6be1e85e972c4bdbbf99d4881d98b0a42021-09-01T00:00:00Zhttps://elifesciences.org/articles/69508https://doaj.org/toc/2050-084XTemporal dynamics of gene expression underpin responses to internal and environmental stimuli. In eukaryotes, regulation of gene induction includes changing chromatin states at target genes and recruiting the transcriptional machinery that includes transcription factors. As one of the most potent defense compounds in Arabidopsis thaliana, camalexin can be rapidly induced by bacterial and fungal infections. Though several transcription factors controlling camalexin biosynthesis genes have been characterized, how the rapid activation of genes in this pathway upon a pathogen signal is enabled remains unknown. By combining publicly available epigenomic data with in vivo chromatin modification mapping, we found that camalexin biosynthesis genes are marked with two epigenetic modifications with opposite effects on gene expression, trimethylation of lysine 27 of histone 3 (H3K27me3) (repression) and acetylation of lysine 18 of histone 3 (H3K18ac) (activation), to form a previously uncharacterized type of bivalent chromatin. Mutants with reduced H3K27me3 or H3K18ac suggested that both modifications were required to determine the timing of gene expression and metabolite accumulation at an early stage of the stress response. Our study indicates that the H3K27me3-H3K18ac bivalent chromatin, which we name as kairostat, plays an important role in controlling the timely induction of gene expression upon stress stimuli in plants.Kangmei ZhaoDeze KongBenjamin JinChristina D SmolkeSeung Yon RheeeLife Sciences Publications Ltdarticlebivalent chromatinregulation of gene expressionbiotic stress responseplant metabolismMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021) |
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bivalent chromatin regulation of gene expression biotic stress response plant metabolism Medicine R Science Q Biology (General) QH301-705.5 |
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bivalent chromatin regulation of gene expression biotic stress response plant metabolism Medicine R Science Q Biology (General) QH301-705.5 Kangmei Zhao Deze Kong Benjamin Jin Christina D Smolke Seung Yon Rhee A novel bivalent chromatin associates with rapid induction of camalexin biosynthesis genes in response to a pathogen signal in Arabidopsis |
| description |
Temporal dynamics of gene expression underpin responses to internal and environmental stimuli. In eukaryotes, regulation of gene induction includes changing chromatin states at target genes and recruiting the transcriptional machinery that includes transcription factors. As one of the most potent defense compounds in Arabidopsis thaliana, camalexin can be rapidly induced by bacterial and fungal infections. Though several transcription factors controlling camalexin biosynthesis genes have been characterized, how the rapid activation of genes in this pathway upon a pathogen signal is enabled remains unknown. By combining publicly available epigenomic data with in vivo chromatin modification mapping, we found that camalexin biosynthesis genes are marked with two epigenetic modifications with opposite effects on gene expression, trimethylation of lysine 27 of histone 3 (H3K27me3) (repression) and acetylation of lysine 18 of histone 3 (H3K18ac) (activation), to form a previously uncharacterized type of bivalent chromatin. Mutants with reduced H3K27me3 or H3K18ac suggested that both modifications were required to determine the timing of gene expression and metabolite accumulation at an early stage of the stress response. Our study indicates that the H3K27me3-H3K18ac bivalent chromatin, which we name as kairostat, plays an important role in controlling the timely induction of gene expression upon stress stimuli in plants. |
| format |
article |
| author |
Kangmei Zhao Deze Kong Benjamin Jin Christina D Smolke Seung Yon Rhee |
| author_facet |
Kangmei Zhao Deze Kong Benjamin Jin Christina D Smolke Seung Yon Rhee |
| author_sort |
Kangmei Zhao |
| title |
A novel bivalent chromatin associates with rapid induction of camalexin biosynthesis genes in response to a pathogen signal in Arabidopsis |
| title_short |
A novel bivalent chromatin associates with rapid induction of camalexin biosynthesis genes in response to a pathogen signal in Arabidopsis |
| title_full |
A novel bivalent chromatin associates with rapid induction of camalexin biosynthesis genes in response to a pathogen signal in Arabidopsis |
| title_fullStr |
A novel bivalent chromatin associates with rapid induction of camalexin biosynthesis genes in response to a pathogen signal in Arabidopsis |
| title_full_unstemmed |
A novel bivalent chromatin associates with rapid induction of camalexin biosynthesis genes in response to a pathogen signal in Arabidopsis |
| title_sort |
novel bivalent chromatin associates with rapid induction of camalexin biosynthesis genes in response to a pathogen signal in arabidopsis |
| publisher |
eLife Sciences Publications Ltd |
| publishDate |
2021 |
| url |
https://doaj.org/article/6be1e85e972c4bdbbf99d4881d98b0a4 |
| work_keys_str_mv |
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