Synthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells

Hua Yao,1,* Jing Li,1,* Yubin Song,2 Hong Zhao,1 Zhenhong Wei,1 Xiuying Li,1 Yongri Jin,3 Bai Yang,2 Jinlan Jiang1 1Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin, P. R. China; 2State Key Laboratory of Supramolecular Structure and Materials, College of...

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Autores principales:	Yao H, Li J, Song Y, Zhao H, Wei Z, Li X, Jin Y, Yang B, Jiang J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	carbon dots ginsenoside Re excitation-dependent fluorescence cell imaging anticancer activity; Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6bef777c866943a88f8cdef66e3dc8e4
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spelling	oai:doaj.org-article:6bef777c866943a88f8cdef66e3dc8e42021-12-02T07:04:49ZSynthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells1178-2013https://doaj.org/article/6bef777c866943a88f8cdef66e3dc8e42018-10-01T00:00:00Zhttps://www.dovepress.com/synthesis-of-ginsenoside-re-based-carbon-dots-applied-for-bioimaging-a-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Hua Yao,1,* Jing Li,1,* Yubin Song,2 Hong Zhao,1 Zhenhong Wei,1 Xiuying Li,1 Yongri Jin,3 Bai Yang,2 Jinlan Jiang1 1Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin, P. R. China; 2State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, Jilin, P. R. China; 3College of Chemistry, Jilin University, Changchun, Jilin, P. R. China *These authors contributed equally to this work Background: Fluorescent carbon-based nanomaterials have promising properties such as biosensing, cell imaging, tracing and drug delivery. However, carbon dots (CDs) with specific inherent biological functions have not been investigated. Ginsenosides are the components with multiple bioactivities found in plants of the genus Panax, which have attracted a lot of attention for their anticancer effect.Materials and methods: In this study, we prepared a kind of novel photoluminescent CDs from ginsenoside Re by one-step hydrothermal synthesis method. The conventional methods including transmission electron microscopy, Fourier transform infrared spectroscopy, HPLC and fluorescence spectrum were used for characterization of CDs. In vitro anticancer effect was investigated by cytotoxicity assay, flow cytometry and Western blot analysis.Results: The as-prepared Re-CDs had an average diameter of 4.6±0.6 nm and excellent luminescent properties. Cellular uptake of Re-CDs was facilitated by their tiny nanosize, with evidence of their bright excitation-dependent fluorescent images. Compared with ginsenoside Re, the Re-CDs showed greater inhibition efficiency of cancer cell proliferation, with lower toxicity to the normal cells. The anticancer activity of Re-CDs was suggested to be associated with the generation of large amount of ROS and the caspase-3 related cell apoptosis.Conclusion: Hopefully, the dual functional Re-CDs, which could both exhibit bioimaging and anticancer effect, are expected to have great potential in future clinical applications. Keywords: carbon dots, ginsenoside Re, excitation-dependent fluorescence, cell imaging, anticancer activityYao HLi JSong YZhao HWei ZLi XJin YYang BJiang JDove Medical Pressarticlecarbon dotsginsenoside Reexcitation-dependent fluorescencecell imaginganticancer activity;Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 6249-6264 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbon dots ginsenoside Re excitation-dependent fluorescence cell imaging anticancer activity; Medicine (General) R5-920
spellingShingle	carbon dots ginsenoside Re excitation-dependent fluorescence cell imaging anticancer activity; Medicine (General) R5-920 Yao H Li J Song Y Zhao H Wei Z Li X Jin Y Yang B Jiang J Synthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells
description	Hua Yao,1,* Jing Li,1,* Yubin Song,2 Hong Zhao,1 Zhenhong Wei,1 Xiuying Li,1 Yongri Jin,3 Bai Yang,2 Jinlan Jiang1 1Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin, P. R. China; 2State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, Jilin, P. R. China; 3College of Chemistry, Jilin University, Changchun, Jilin, P. R. China *These authors contributed equally to this work Background: Fluorescent carbon-based nanomaterials have promising properties such as biosensing, cell imaging, tracing and drug delivery. However, carbon dots (CDs) with specific inherent biological functions have not been investigated. Ginsenosides are the components with multiple bioactivities found in plants of the genus Panax, which have attracted a lot of attention for their anticancer effect.Materials and methods: In this study, we prepared a kind of novel photoluminescent CDs from ginsenoside Re by one-step hydrothermal synthesis method. The conventional methods including transmission electron microscopy, Fourier transform infrared spectroscopy, HPLC and fluorescence spectrum were used for characterization of CDs. In vitro anticancer effect was investigated by cytotoxicity assay, flow cytometry and Western blot analysis.Results: The as-prepared Re-CDs had an average diameter of 4.6±0.6 nm and excellent luminescent properties. Cellular uptake of Re-CDs was facilitated by their tiny nanosize, with evidence of their bright excitation-dependent fluorescent images. Compared with ginsenoside Re, the Re-CDs showed greater inhibition efficiency of cancer cell proliferation, with lower toxicity to the normal cells. The anticancer activity of Re-CDs was suggested to be associated with the generation of large amount of ROS and the caspase-3 related cell apoptosis.Conclusion: Hopefully, the dual functional Re-CDs, which could both exhibit bioimaging and anticancer effect, are expected to have great potential in future clinical applications. Keywords: carbon dots, ginsenoside Re, excitation-dependent fluorescence, cell imaging, anticancer activity
format	article
author	Yao H Li J Song Y Zhao H Wei Z Li X Jin Y Yang B Jiang J
author_facet	Yao H Li J Song Y Zhao H Wei Z Li X Jin Y Yang B Jiang J
author_sort	Yao H
title	Synthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells
title_short	Synthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells
title_full	Synthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells
title_fullStr	Synthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells
title_full_unstemmed	Synthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells
title_sort	synthesis of ginsenoside re-based carbon dots applied for bioimaging and effective inhibition of cancer cells
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/6bef777c866943a88f8cdef66e3dc8e4
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Synthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells

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