Dysbiosis in chronic periodontitis: Key microbial players and interactions with the human host

Abstract Periodontitis is an extremely prevalent disease worldwide and is driven by complex dysbiotic microbiota. Here we analyzed the transcriptional activity of the periodontal pocket microbiota from all domains of life as well as the human host in health and chronic periodontitis. Bacteria showed...

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Autores principales:	Zhi-Luo Deng, Szymon P. Szafrański, Michael Jarek, Sabin Bhuju, Irene Wagner-Döbler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6bfd20346fc842dc871004fcc8a8f098
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spelling	oai:doaj.org-article:6bfd20346fc842dc871004fcc8a8f0982021-12-02T15:05:13ZDysbiosis in chronic periodontitis: Key microbial players and interactions with the human host10.1038/s41598-017-03804-82045-2322https://doaj.org/article/6bfd20346fc842dc871004fcc8a8f0982017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03804-8https://doaj.org/toc/2045-2322Abstract Periodontitis is an extremely prevalent disease worldwide and is driven by complex dysbiotic microbiota. Here we analyzed the transcriptional activity of the periodontal pocket microbiota from all domains of life as well as the human host in health and chronic periodontitis. Bacteria showed strong enrichment of 18 KEGG functional modules in chronic periodontitis, including bacterial chemotaxis, flagellar assembly, type III secretion system, type III CRISPR-Cas system, and two component system proteins. Upregulation of these functions was driven by the red-complex pathogens and candidate pathogens, e.g. Filifactor alocis, Prevotella intermedia, Fretibacterium fastidiosum and Selenomonas sputigena. Nine virulence factors were strongly up-regulated, among them the arginine deiminase arcA from Porphyromonas gingivalis and Mycoplasma arginini. Viruses and archaea accounted for about 0.1% and 0.22% of total putative mRNA reads, respectively, and a protozoan, Entamoeba gingivalis, was highly enriched in periodontitis. Fourteen human transcripts were enriched in periodontitis, including a gene for a ferric iron binding protein, indicating competition with the microbiota for iron, and genes associated with cancer, namely nucleolar phosphoprotein B23, ankyrin-repeat domain 30B-like protein and beta-enolase. The data provide evidence on the level of gene expression in vivo for the potentially severe impact of the dysbiotic microbiota on human health.Zhi-Luo DengSzymon P. SzafrańskiMichael JarekSabin BhujuIrene Wagner-DöblerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhi-Luo Deng Szymon P. Szafrański Michael Jarek Sabin Bhuju Irene Wagner-Döbler Dysbiosis in chronic periodontitis: Key microbial players and interactions with the human host
description	Abstract Periodontitis is an extremely prevalent disease worldwide and is driven by complex dysbiotic microbiota. Here we analyzed the transcriptional activity of the periodontal pocket microbiota from all domains of life as well as the human host in health and chronic periodontitis. Bacteria showed strong enrichment of 18 KEGG functional modules in chronic periodontitis, including bacterial chemotaxis, flagellar assembly, type III secretion system, type III CRISPR-Cas system, and two component system proteins. Upregulation of these functions was driven by the red-complex pathogens and candidate pathogens, e.g. Filifactor alocis, Prevotella intermedia, Fretibacterium fastidiosum and Selenomonas sputigena. Nine virulence factors were strongly up-regulated, among them the arginine deiminase arcA from Porphyromonas gingivalis and Mycoplasma arginini. Viruses and archaea accounted for about 0.1% and 0.22% of total putative mRNA reads, respectively, and a protozoan, Entamoeba gingivalis, was highly enriched in periodontitis. Fourteen human transcripts were enriched in periodontitis, including a gene for a ferric iron binding protein, indicating competition with the microbiota for iron, and genes associated with cancer, namely nucleolar phosphoprotein B23, ankyrin-repeat domain 30B-like protein and beta-enolase. The data provide evidence on the level of gene expression in vivo for the potentially severe impact of the dysbiotic microbiota on human health.
format	article
author	Zhi-Luo Deng Szymon P. Szafrański Michael Jarek Sabin Bhuju Irene Wagner-Döbler
author_facet	Zhi-Luo Deng Szymon P. Szafrański Michael Jarek Sabin Bhuju Irene Wagner-Döbler
author_sort	Zhi-Luo Deng
title	Dysbiosis in chronic periodontitis: Key microbial players and interactions with the human host
title_short	Dysbiosis in chronic periodontitis: Key microbial players and interactions with the human host
title_full	Dysbiosis in chronic periodontitis: Key microbial players and interactions with the human host
title_fullStr	Dysbiosis in chronic periodontitis: Key microbial players and interactions with the human host
title_full_unstemmed	Dysbiosis in chronic periodontitis: Key microbial players and interactions with the human host
title_sort	dysbiosis in chronic periodontitis: key microbial players and interactions with the human host
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6bfd20346fc842dc871004fcc8a8f098
work_keys_str_mv	AT zhiluodeng dysbiosisinchronicperiodontitiskeymicrobialplayersandinteractionswiththehumanhost AT szymonpszafranski dysbiosisinchronicperiodontitiskeymicrobialplayersandinteractionswiththehumanhost AT michaeljarek dysbiosisinchronicperiodontitiskeymicrobialplayersandinteractionswiththehumanhost AT sabinbhuju dysbiosisinchronicperiodontitiskeymicrobialplayersandinteractionswiththehumanhost AT irenewagnerdobler dysbiosisinchronicperiodontitiskeymicrobialplayersandinteractionswiththehumanhost
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Dysbiosis in chronic periodontitis: Key microbial players and interactions with the human host

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