LC–MS Based Draft Map of the Arabidopsis thaliana Nuclear Proteome and Protein Import in Pattern Triggered Immunity

Despite its central role as the ark of genetic information and gene expression the plant nucleus is surprisingly understudied. We isolated nuclei from the Arabidopsis thaliana dark grown cell culture left untreated and treated with flg22 and nlp20, two elicitors of pattern triggered immunity (PTI) i...

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Autores principales:	Mohamed Ayash, Mohammad Abukhalaf, Domenika Thieme, Carsten Proksch, Mareike Heilmann, Martin Hartmut Schattat, Wolfgang Hoehenwarter
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	proteomics nucleus Arabidopsis protein trafficking pattern triggered immunity Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/6c00ae218c4f464d8d4b586f1839b7a0
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spelling	oai:doaj.org-article:6c00ae218c4f464d8d4b586f1839b7a02021-11-08T16:20:53ZLC–MS Based Draft Map of the Arabidopsis thaliana Nuclear Proteome and Protein Import in Pattern Triggered Immunity1664-462X10.3389/fpls.2021.744103https://doaj.org/article/6c00ae218c4f464d8d4b586f1839b7a02021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.744103/fullhttps://doaj.org/toc/1664-462XDespite its central role as the ark of genetic information and gene expression the plant nucleus is surprisingly understudied. We isolated nuclei from the Arabidopsis thaliana dark grown cell culture left untreated and treated with flg22 and nlp20, two elicitors of pattern triggered immunity (PTI) in plants, respectively. An liquid chromatography mass spectrometry (LC–MS) based discovery proteomics approach was used to measure the nuclear proteome fractions. An enrichment score based on the relative abundance of cytoplasmic, mitochondrial and Golgi markers in the nuclear protein fraction allowed us to curate the nuclear proteome producing high quality catalogs of around 3,000 nuclear proteins under untreated and both PTI conditions. The measurements also covered low abundant proteins including more than 100 transcription factors and transcriptional co-activators. We disclose a list of several hundred potentially dual targeted proteins including proteins not yet found before for further study. Protein import into the nucleus in plant immunity is known. Here we sought to gain a broader impression of this phenomenon employing our proteomics data and found 157 and 73 proteins to possibly be imported into the nucleus upon stimulus with flg22 and nlp20, respectively. Furthermore, the abundance of 93 proteins changed significantly in the nucleus following elicitation of immunity. These results suggest promiscuous ribosome assembly and a role of prohibitins and cytochrome C in the nucleus in PTI.Mohamed AyashMohammad AbukhalafDomenika ThiemeCarsten ProkschMareike HeilmannMartin Hartmut SchattatWolfgang HoehenwarterFrontiers Media S.A.articleproteomicsnucleusArabidopsisprotein traffickingpattern triggered immunityPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	proteomics nucleus Arabidopsis protein trafficking pattern triggered immunity Plant culture SB1-1110
spellingShingle	proteomics nucleus Arabidopsis protein trafficking pattern triggered immunity Plant culture SB1-1110 Mohamed Ayash Mohammad Abukhalaf Domenika Thieme Carsten Proksch Mareike Heilmann Martin Hartmut Schattat Wolfgang Hoehenwarter LC–MS Based Draft Map of the Arabidopsis thaliana Nuclear Proteome and Protein Import in Pattern Triggered Immunity
description	Despite its central role as the ark of genetic information and gene expression the plant nucleus is surprisingly understudied. We isolated nuclei from the Arabidopsis thaliana dark grown cell culture left untreated and treated with flg22 and nlp20, two elicitors of pattern triggered immunity (PTI) in plants, respectively. An liquid chromatography mass spectrometry (LC–MS) based discovery proteomics approach was used to measure the nuclear proteome fractions. An enrichment score based on the relative abundance of cytoplasmic, mitochondrial and Golgi markers in the nuclear protein fraction allowed us to curate the nuclear proteome producing high quality catalogs of around 3,000 nuclear proteins under untreated and both PTI conditions. The measurements also covered low abundant proteins including more than 100 transcription factors and transcriptional co-activators. We disclose a list of several hundred potentially dual targeted proteins including proteins not yet found before for further study. Protein import into the nucleus in plant immunity is known. Here we sought to gain a broader impression of this phenomenon employing our proteomics data and found 157 and 73 proteins to possibly be imported into the nucleus upon stimulus with flg22 and nlp20, respectively. Furthermore, the abundance of 93 proteins changed significantly in the nucleus following elicitation of immunity. These results suggest promiscuous ribosome assembly and a role of prohibitins and cytochrome C in the nucleus in PTI.
format	article
author	Mohamed Ayash Mohammad Abukhalaf Domenika Thieme Carsten Proksch Mareike Heilmann Martin Hartmut Schattat Wolfgang Hoehenwarter
author_facet	Mohamed Ayash Mohammad Abukhalaf Domenika Thieme Carsten Proksch Mareike Heilmann Martin Hartmut Schattat Wolfgang Hoehenwarter
author_sort	Mohamed Ayash
title	LC–MS Based Draft Map of the Arabidopsis thaliana Nuclear Proteome and Protein Import in Pattern Triggered Immunity
title_short	LC–MS Based Draft Map of the Arabidopsis thaliana Nuclear Proteome and Protein Import in Pattern Triggered Immunity
title_full	LC–MS Based Draft Map of the Arabidopsis thaliana Nuclear Proteome and Protein Import in Pattern Triggered Immunity
title_fullStr	LC–MS Based Draft Map of the Arabidopsis thaliana Nuclear Proteome and Protein Import in Pattern Triggered Immunity
title_full_unstemmed	LC–MS Based Draft Map of the Arabidopsis thaliana Nuclear Proteome and Protein Import in Pattern Triggered Immunity
title_sort	lc–ms based draft map of the arabidopsis thaliana nuclear proteome and protein import in pattern triggered immunity
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6c00ae218c4f464d8d4b586f1839b7a0
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LC–MS Based Draft Map of the Arabidopsis thaliana Nuclear Proteome and Protein Import in Pattern Triggered Immunity

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