The effect of fish matrix on colloidal gold immunochromatographic assay of antibiotics: the source of interference and removal method

The matrix effect is a serious problem for the immunoassay of antibiotic residues, which greatly restricts its real application in fishery products as well as other foodstuffs. Till now the related information on the colloidal gold immunochromatographic assay (GICA) is still limited. Therefore, five...

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Autores principales:	Xiaoxiao Wang, Hong Lin, Xueli Qu, Hongwei Zheng, Jianxin Sui, Limin Cao
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	matrix effect fish antibiotics immunochromatographic assay magnesium Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/6c070be329b3414290216032538207ad
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spelling	oai:doaj.org-article:6c070be329b3414290216032538207ad2021-11-26T11:19:46ZThe effect of fish matrix on colloidal gold immunochromatographic assay of antibiotics: the source of interference and removal method0954-01051465-344310.1080/09540105.2018.1483900https://doaj.org/article/6c070be329b3414290216032538207ad2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1483900https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443The matrix effect is a serious problem for the immunoassay of antibiotic residues, which greatly restricts its real application in fishery products as well as other foodstuffs. Till now the related information on the colloidal gold immunochromatographic assay (GICA) is still limited. Therefore, five species of fish as samples, and the interference of main matrix components on the analysis of ciprofloxacin was investigated. Some water-soluble proteins were demonstrated to have significant affluence on GICA. Moreover, magnesium is also an important source of the matrix interference was revealed for the first time, and its interaction with some immunoglobin (Ig)G was assumed to be a possible reason for this. Based on these results, corresponding approaches for elimination of the interference were also explored. The matrix interference was fully eliminated and the sensitivity of GICA was increased about 8 times for the quantitative analysis of ciprofloxacin via acid pre-treatment followed by pH adjustment.Xiaoxiao WangHong LinXueli QuHongwei ZhengJianxin SuiLimin CaoTaylor & Francis Grouparticlematrix effectfishantibioticsimmunochromatographic assaymagnesiumAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 898-910 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	matrix effect fish antibiotics immunochromatographic assay magnesium Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	matrix effect fish antibiotics immunochromatographic assay magnesium Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Xiaoxiao Wang Hong Lin Xueli Qu Hongwei Zheng Jianxin Sui Limin Cao The effect of fish matrix on colloidal gold immunochromatographic assay of antibiotics: the source of interference and removal method
description	The matrix effect is a serious problem for the immunoassay of antibiotic residues, which greatly restricts its real application in fishery products as well as other foodstuffs. Till now the related information on the colloidal gold immunochromatographic assay (GICA) is still limited. Therefore, five species of fish as samples, and the interference of main matrix components on the analysis of ciprofloxacin was investigated. Some water-soluble proteins were demonstrated to have significant affluence on GICA. Moreover, magnesium is also an important source of the matrix interference was revealed for the first time, and its interaction with some immunoglobin (Ig)G was assumed to be a possible reason for this. Based on these results, corresponding approaches for elimination of the interference were also explored. The matrix interference was fully eliminated and the sensitivity of GICA was increased about 8 times for the quantitative analysis of ciprofloxacin via acid pre-treatment followed by pH adjustment.
format	article
author	Xiaoxiao Wang Hong Lin Xueli Qu Hongwei Zheng Jianxin Sui Limin Cao
author_facet	Xiaoxiao Wang Hong Lin Xueli Qu Hongwei Zheng Jianxin Sui Limin Cao
author_sort	Xiaoxiao Wang
title	The effect of fish matrix on colloidal gold immunochromatographic assay of antibiotics: the source of interference and removal method
title_short	The effect of fish matrix on colloidal gold immunochromatographic assay of antibiotics: the source of interference and removal method
title_full	The effect of fish matrix on colloidal gold immunochromatographic assay of antibiotics: the source of interference and removal method
title_fullStr	The effect of fish matrix on colloidal gold immunochromatographic assay of antibiotics: the source of interference and removal method
title_full_unstemmed	The effect of fish matrix on colloidal gold immunochromatographic assay of antibiotics: the source of interference and removal method
title_sort	effect of fish matrix on colloidal gold immunochromatographic assay of antibiotics: the source of interference and removal method
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/6c070be329b3414290216032538207ad
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The effect of fish matrix on colloidal gold immunochromatographic assay of antibiotics: the source of interference and removal method

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